Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-10T12:51:10.519Z Has data issue: false hasContentIssue false
  • English
  • Français

The evaluation of salivary gland tumours using proliferating cell nuclear antigen

Published online by Cambridge University Press:  29 June 2007

N. J. Trendell-Smith ,
J. Oates  and
J. Crocker
N. J. Trendell-Smith*
Affiliation:
Department of Histopathology, Birmingham Heartlands Hospital, Bordesley Green East, Birmingham, UK.
J. Oates
Affiliation:
Department of Histopathology, Birmingham Heartlands Hospital, Bordesley Green East, Birmingham, UK.
J. Crocker
Affiliation:
Department of Histopathology, Birmingham Heartlands Hospital, Bordesley Green East, Birmingham, UK.
*
Address for correspondence: N. J. Trendell-Smith, Department of Histopathology, Charing Cross and Westminster Medical School, Charing Cross Hospital, London W6 8RF.
Get access Rights & Permissions [Opens in a new window]

Abstract

In order to assess its discriminating and prognostic value, we studied the immunoreactivity for proliferating cell nuclear antigen (PCNA) in tissue from 52 human salivary gland tumours using the murine monoclonal antibody PC10.

The PCNA percentage count, namely, the average number of positive nuclei counted per 100 randomly selected tumour cells was recorded for each tumour. Anaplastic carcinoma was used as a positive control and histologically ‘normal’ salivary gland and tonsil served as a negative control.

A PCNA count of 30 per cent was postulated to predict malignancy within a given salivary gland tumour i.e. a PCNA count of 30 per cent or above would indicate malignant potential. This gave a sensitivity of 96.9 per cent and a specificity of 95.2 per cent and a positive predictive value of determining malignancy of 96.8 per cent.

We conclude that PCNA immunoreactivity is useful in discriminating between benign and malignant salivary gland tumours and that it may have prognostic value in this diverse group of neoplasms.

Keywords

Salivary gland neoplasmsAntigens, differentiationImmunohistochemistry
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 111 , Issue 6 , June 1997 , pp. 551 - 555
Copyright
Copyright © JLO (1984) Limited 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Battersby, S., Anderson, T. J. (1990) Correlation of proliferative activity in breast tissue using PCNA/cyclin. Human Pathology 21: 781.CrossRefGoogle ScholarPubMed
Benjamin, D. R., Gown, A. M. (1991) Aberrant cytoplasmic expression of PCNA in Hodgkin's disease. American Journal of Surgical Pathology 15; 764–768.CrossRefGoogle ScholarPubMed
Crocker, J., Jenkins, R., Campbell, J., Fuggle, W. J., Shah, V. M. (1985) Immunohistochemical determination of S100 protein in salivary gland neoplasms. Journal of Pathology 146: 115–121.CrossRefGoogle ScholarPubMed
Hall, P. A., Levison, D. A. (1990) Assessment of cell proliferation in histological material. Journal of Clinical Pathology 43: 184–192.CrossRefGoogle Scholar
Hall, P. A., Levison, D. A., Woods, A. L., Yu, C. W., Kellock, D. B., Watkins, J. A., Barnes, D. M., Gillett, C. E., Camplejohn, R., Dover, R., Waseem, N. H., Lane, D. P. (1990) Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. Journal of Pathology 162: 285–294.CrossRefGoogle ScholarPubMed
Hickman, R. E., Cawson, R. A., Duffy, S. W. (1984) The prognosis of specific types of salivary gland tumours. Cancer 54: 1620–1624.3.0.CO;2-I>CrossRefGoogle Scholar
Jain, S., Filipe, N. I., Hall, P. A., Waseem, N. H., Lane, D. P., Levison, D. A. (1991) Prognostic value of proliferating cell nuclear antigen in gastric carcinoma. Journal of Clinical Pathology 44: 655–659.CrossRefGoogle ScholarPubMed
Linden, M. D., Torres, F. X., Kubus, J., Zarbo, R. (1992) Editorial. Clinical implications of morphologic and immunohistochemical assessment of cell proliferation. American Journal of Clinical Pathology 97: (Suppl) S4–S13.Google Scholar
Louis, D. N., Edgerton, S., Thor, A. D., Hedley-Whyte, E. T. (1991) Proliferating cell nuclear antigen and Ki67 immunohistochemistry in brain tumours: a comparative study. Acta Neuropathologica 81(6): 675–679.CrossRefGoogle ScholarPubMed
Mathews, M. B., Bernstein, R. M., Franza, B. R. J., Garrels, J. I. (1984) Identity of the proliferating cell nuclear antigen and cyclin. Nature 309: 374–376.CrossRefGoogle ScholarPubMed
McCormick, D., Hall, P. A. (1992) The complexities of proliferating cell nuclear antigen. Histopathology 21: 591–594.CrossRefGoogle ScholarPubMed
Morgan, D. W., Crocker, J., Watts, A., Shenoi, P. M. (1988) Salivary gland tumours studied by means of the AgNOR technique. Histopathology 13: 553–594.CrossRefGoogle ScholarPubMed
Murakami, M., Ohtani, I., Hojo, H., Wakasa, H. (1992) Immunohistochemical evaluation of Ki67: an application to salivary gland tumours. Journal of Laryngology and Otology 106: 35–38.CrossRefGoogle ScholarPubMed
Nakazato, Y., Ishida, Y., Takahishi, K., Suzuki, K. (1985) Immunohistochemical distribution of S100 protein and GFAP in normal and neoplastic salivary glands. Virchows Archives 405: 299–310.CrossRefGoogle ScholarPubMed
Prelich, G., Tan, C. K., Kotsura, M., Mathews, M. B., So, A. G., Downey, K. M., Stillman, B. (1987) Functional identity of proliferating cell nuclear antigen and a DNA polymerase-delta auxillary protein. Nature 326: 517–520.CrossRefGoogle Scholar
Ramael, M., Jacobs, W., Weyler, J., Van Meerbeeck, J., Bialasiewicz, P., Van Den Bossche, J. (1994) Proliferation in malignant mesothelioma as determined by mitotic counts and immunoreactivity for proliferating cell nuclear antigen. Journal of Pathology 172: 247–253.CrossRefGoogle ScholarPubMed
Siefert, G., Brocheriou, C., Cardesa, A., Eveson, J. W. (1990) World Health Organization International histological classification of tumours: tentative histological classification of salivary gland tumours. World Health Organization Publications.CrossRefGoogle Scholar
Woods, A. L., Hanby, A. M., Hall, P. A., Shepherd, N. A., Waseem, N. H., Lane, D. P., Levison, D. A. (1990) The prognostic value of proliferating cell nuclear antigen immunostaining in gastrointestinal lymphomas (abstract). Journal of Pathology 161: 342A.Google Scholar
Wynford-Thomas, D. (1992) P53 in tumour pathology: can we trust immunohistochemistry? Journal of Pathology 166: 329–330.CrossRefGoogle Scholar
Young, J. A. (1994) Diagnostic problems in fine needle aspiration cytology of the salivary gland. Journal of Clinical Pathology 47: 193–198.CrossRefGoogle Scholar
Yu, C., Hall, P. A., Fletcher, C. D. M., Camplejohn, R., Waseem, N., Lane, P. D., Levison, D. A. (1991) Immunohistochemical staining with a monoclonal antibody to proliferating cell nuclear antigen may be a good indicator of prognosis in haemangiopericytomas. Histopathology 19: 29–34.CrossRefGoogle Scholar