Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-27T08:37:31.715Z Has data issue: false hasContentIssue false
  • English
  • Français

Human papilloma viruses: a study of their prevalence in the normal larynx

Published online by Cambridge University Press:  29 June 2007

Desmond A. Nunez ,
Siân M. Astley ,
Fraser A. Lewis  and
Michael Wells
Desmond A. Nunez*
Affiliation:
Department of Otolaryngology, Leicester Royal Infirmary, Department of Clinical Medicine, University of Leeds.
Siân M. Astley
Affiliation:
Department of Otolaryngology, Academic Unit of Pathology, Department of Clinical Medicine, University of Leeds.
Fraser A. Lewis
Affiliation:
Department of Otolaryngology, Academic Unit of Pathology, Department of Clinical Medicine, University of Leeds.
Michael Wells
Affiliation:
Department of Otolaryngology, Academic Unit of Pathology, Department of Clinical Medicine, University of Leeds.
*
D. A. Nunez, F.R.C.S., ENT Department, Royal Infirmary, Leicester LE1 5WW. Fax: 0533 586082.
Get access Rights & Permissions [Opens in a new window]

Abstract

The association of human papilloma viruses (HPV) with laryngopharyngeal squamous cell carcinoma is under investigation. The suitability of control tissue in the reported series, invariably obtained from histologically normal tissue adjacent to a squamous cell carcinoma or from patients with benign laryngopharyngeal disease, is questionable. The present study determined the prevalence of HPV in a series of normal larynges.

Twelve autopsy larynges were collected. DNA was obtained by SDS proteinase K digestion. Evidence of HPV infection was documented by the polymerase chain reaction using oligonulceotide primers complementary to sequences in the E6 region of HPV types 11, 16 and 18.

Four female and eight male larynges, mean age 65 years (sd=16 years) were collected 72 hours postmortem (median value). HPV type 11 was isolated from three specimens. A 25 per cent prevalence rate for HPV 11 was found. No other HPV types were isolated.

Keywords

DNALarynxPolymerase chain reactionViruses
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 108 , Issue 4 , April 1994 , pp. 319 - 320
Copyright
Copyright © JLO (1984) Limited 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Presented at the Otolaryngology Research Society Meeting, London, September 1992.

References

Brandasma, J. L., Abramson, A. L. (1989) Association of papillomavirus with cancers of the head and neck. Archives of Otolaryngology, Head and Neck Surgery 115: 621–625.Google Scholar
Bryan, R. L., Bevan, I. S., Crocker, J., Young, L. S. (1990) Detection of HPV 6 and 11 in tumours of the upper respiratory tract using the polymerase chain reaction. Clinical Otolaryngology 15: 177–180.CrossRefGoogle ScholarPubMed
Dekmezian, R. H., Batsakis, J. G., Goepfert, H. (1987) In situ hybridisation of papillomavirus DNA in head and neck squamous cell carcinoma. Archives of tolaryngology, Head and Neck Surgery 113: 819–821.CrossRefGoogle Scholar
Griffin, N. R., Bevan, I. S., Lewis, F. A., Wells, M, Young, L. S. (1990) Demonstration of multiple HPV types in normal cervix and in cervical squamous cell carcinoma using the polymerasechain reaction on paraffin wax embedded material. Journal of Clinical Pathology 43: 52–56.CrossRefGoogle Scholar
Hoshikawa, T., Nakajima, T., Uhara, H., Gotoh, M, Shimosato, Y., Tsutsumi, K., Ono, I., Ebihara, S. (1990) Detection of human papillomavirus DNA in laryngeal squamous cell carcinomas by polymerase chain reaction. Laryngoscope 100: 647–650.CrossRefGoogle ScholarPubMed
Kashima, H., Mounts, P., Kuhajda, F., Loury, M. (1986) Demonstration of human papillomavirus capsid antigen in carcinomain situ of the larynx. Annals of Otology, Rhinology and Laryngology 95: 603–607.CrossRefGoogle Scholar
Kiyabu, M. T., Shibata, D., Arnheim, N., Martin, W. J., Fitzgibbons, P. L. (1989) Detection of human papillomavirus in formalin-fixed invasive squamous carcinomas using the polymerase chain reaction. American Journal of Surgical Pathology 13: 221–224.CrossRefGoogle ScholarPubMed
Morgan, D. W., Abdullah, V., Quiney, R., Myint, S. (1991) Human papilloma virus and carcinoma of the laryngopharynx. Journal of Laryngology and Otology 105: 288–290.CrossRefGoogle ScholarPubMed
Ogura, H., Watanabe, S., Fukushima, K., Masuda, Y, Fujiwara, T., Yabe, Y. (1991) Presence of human papillomavirus type 18 DNA in a pharyngeal and a laryngeal carcinoma. Japanese Journal of Cancer Research 82: 1184–1186.Google Scholar
Terry, R. M., Lewis, F. A., Griffiths, S., Wells, M., Bird, C. C. (1987) demonstration of human papillomavirus types 6 and 11 in juvenile laryngeal papillomatosis by in situ hybridisation. Journal of Pathology 153: 245–248.CrossRefGoogle Scholar