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In vitro induction and differentiation of newborn guinea pig hippocampus neural stem cells into cells resembling inner hair cells, using artificial perilymph

Published online by Cambridge University Press:  07 June 2011

Y Wang  and
M-M Dong
Y Wang
Affiliation:
Department of Otolaryngology, First Affiliated Hospital of Zhengzhou University, China
M-M Dong*
Affiliation:
Department of Otolaryngology, First Affiliated Hospital of Zhengzhou University, China
*
Address for correspondence: Dr Ming-Min Dong, 1 Constructive East Road, Zhengzhou City, China 450052 Fax: +86 024 23380681 E-mail: kaishitengfei@163.com
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Abstract

Objective:

To investigate whether artificial perilymph can induce neural stem cells, derived from the hippocampus of newborn guinea pigs, to differentiate into inner ear hair cells, in vitro.

Methods:

Primary neural stem cells derived from the hippocampus of newborn guinea pigs were incubated in medium containing either 10 per cent fetal bovine serum or 5, 10 or 15 per cent artificial perilymph, for three weeks. Differentiated cells were identified using immunofluorescence, Western blot and scanning electron microscopy.

Results:

Both fetal bovine serum and artificial perilymph induced the neural stem cells to differentiate into cells with hair-cell-specific antibodies.

Conclusion:

Neural stem cells can survive in both fetal bovine serum and artificial perilymph, and within these media can differentiate into cells with hair-cell-specific antibodies. This provides an experimental basis for transplantation of neural stem cells into the inner ear.

Keywords

Ear, InnerStem CellsHair Cells, AuditoryCell DifferentiationPerilymphGuinea Pig
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 125 , Issue 8 , August 2011 , pp. 771 - 775
Copyright
Copyright © JLO (1984) Limited 2011

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