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Intracellular cytokine expression in invasive fungal sinusitis and its impact on patient outcome

Published online by Cambridge University Press:  02 March 2022

P Verma Open the ORCID record for P Verma [Opens in a new window] ,
K Sikka ,
H Verma Open the ORCID record for H Verma [Opens in a new window] ,
A Thakar ,
D K Mitra ,
A Saurabh  and
G Singh
P Verma
Affiliation:
Department of ENT, All India Institute of Medical Sciences, New Delhi, India
K Sikka*
Affiliation:
Department of ENT, All India Institute of Medical Sciences, New Delhi, India
H Verma
Affiliation:
Department of ENT, All India Institute of Medical Sciences, New Delhi, India
A Thakar
Affiliation:
Department of ENT, All India Institute of Medical Sciences, New Delhi, India
D K Mitra
Affiliation:
Department of Transplant Immunology and Immunogenetics, All India Institute of Medical Sciences, New Delhi, India
A Saurabh
Affiliation:
Department of Transplant Immunology and Immunogenetics, All India Institute of Medical Sciences, New Delhi, India
G Singh
Affiliation:
Division of Mycology, Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India
*
Author for correspondence: Dr Kapil Sikka, Room 4063, ENT Office, Academic Block, All India Institute of Medical Sciences, New Delhi110029, India E-mail: kapil_sikka@yahoo.com
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Abstract

Background

Cell-mediated immunity plays an important role in host defence against fungal pathogens, regulated by differentiation of lymphocytes towards T-helper 1 or 2 cells. This study reports intracellular cytokine variation in terms of invasive fungal sinusitis type and outcome.

Methods

The mononuclear leukocytes of 15 patients with invasive fungal sinusitis (mucormycosis in 8, aspergillus in 7) were stained with antibodies against intracellular cytokines, after fungal antigen stimulation and culture, and immunophenotyped. Patients were followed up for six months, with clinical course categorised as improvement, worsening or death.

Results

The mean percentages of mononuclear cells producing interleukins 4, 5, 10 and 12, and interferon-γ, in the mucormycosis group were 0.575, 0.284, 8.661, 4.460 and 1.134, respectively, while percentages in the aspergillosis group were 0.233, 0.492, 4.196, 4.466 and 1.533. Cells producing interleukin 4 and 10 were higher in the mucormycosis group, while those producing interleukin-12 and interferon-γ were lower. Cells producing interleukins 4 and 12 were higher in patients with a poor outcome (p-values of 0.0662 and 0.0373, respectively), while those producing interferon-γ were lower (p = 0.0864).

Conclusion

Adaptive cell-mediated immunity is expressed differently in two categories of invasive fungal sinusitis, and the cytokine expression pattern is related to prognosis.

Keywords

Invasive MycosesCell-Mediated ImmunityFlow CytometryHelper T-CellsParanasal Sinus DiseasesTh1-Th2 Cytokine Balance
Type
Main Article
Information
The Journal of Laryngology & Otology , Volume 136 , Issue 9 , September 2022 , pp. 861 - 865
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of J.L.O. (1984) LIMITED

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Footnotes

Dr K Sikka takes responsibility for the integrity of the content of the paper

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