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The localization and specificity of guinea pig inner ear antigenic epitopes

Published online by Cambridge University Press:  29 June 2007

Ming-Yu Cao ,
Michel Gersdorff ,
Naïma Deggouj  and
Jean-Paul Tomasi
Ming-Yu Cao*
Affiliation:
Department of Otorhinolaryngology, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Brussels, Belgium. Department of Laboratory of Autoimmunity, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Brussels, Belgium.
Michel Gersdorff
Affiliation:
Department of Otorhinolaryngology, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Brussels, Belgium.
Naïma Deggouj
Affiliation:
Department of Otorhinolaryngology, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Brussels, Belgium.
Jean-Paul Tomasi
Affiliation:
Department of Laboratory of Autoimmunity, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Brussels, Belgium.
*
Address for correspondence: Dr Ming-Yu Cao, Laboratoire d'Auto-immunité, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Ave. Hippocrate 53, 1200 Brussels, Belgium. Fax: +32-2-76 9428.
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Abstract

In this study, we investigated the relative localization of some antigenic epitopes in the inner ear. The inner ear protein antigens were extracted from various parts of the guinea pig inner ear. Brain, kidney, lung, heart and liver extracts were also obtained. We found by SDS-polyacrylamide gel electrophoresis that total inner ear extracts separated into three high concentration polypeptide bands with molecular weights of approximately 30, 42, 58 kd and three low density bands of 20,25 and 35 kd. The 30 kd band was found mainly in the extract of the spiral ganglion and the acoustic nerve in the modiolus. The 42 and 58 kd bands were detected in the extract of the spiral ligament and the stria vascularis. The Organ of Corti and the basilar membrane extract gave rise to three bands of 30,42 and 58 kd. Twenty-eight of the 75 sera from patients with inner ear disease reacted with the 30 and 58 kd bands of the inner ear protein extracts by immunoblotting. Sixteen of these 28 positive sera were then used to probe immunoblots of the brain, kidney, lung, heart and liver extracts. The 58 kd band was also found in protein extracts of the brain, the lung and the liver. This study suggests that the 30 kd antigenic epitope may be mainly related to the acoustic nerve and that the 58 kd antigenic epitope is not cochlear specific.

Keywords

CochleaHearing loss, SensorineuralAutoantibodiesImmunoblotting
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 109 , Issue 1 , January 1995 , pp. 19 - 23
Copyright
Copyright © JLO (1984) Limited 1995

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Footnotes

Presented at the International Academic Conference oflmmunobiology in Otology, Rhinology and Laryngology in Oita, Japan, April 4–7 1994.

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