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Mast cell quantitation in nasal polyps, sinus mucosa and nasal turbinate mucosa

Published online by Cambridge University Press:  29 June 2007

Hirokuni Otsuka ,
Kimihiro Ohkubo ,
Harumi Seki ,
Masaki Ohnishi  and
Terumichi Fujikura
Hirokuni Otsuka*
Affiliation:
Department of Otorhinolaryngology, Nippon Medical School, Dai 2 Hospital, Kawasaki City, Kanagawa, Japan.
Kimihiro Ohkubo
Affiliation:
Department of Otorhinolaryngology, Nippon Medical School, Dai 2 Hospital, Kawasaki City, Kanagawa, Japan.
Harumi Seki
Affiliation:
Department of Otorhinolaryngology, Nippon Medical School, Dai 2 Hospital, Kawasaki City, Kanagawa, Japan.
Masaki Ohnishi
Affiliation:
Department of Otorhinolaryngology, Nippon Medical School, Dai 2 Hospital, Kawasaki City, Kanagawa, Japan.
Terumichi Fujikura
Affiliation:
Department of Otorhinolaryngology, Nippon Medical School, Dai 2 Hospital, Kawasaki City, Kanagawa, Japan.
*
Hirokuni Otsuka, M.D., Department of Otorhinolaryngology, Nippon Medical School, Dai 2 Hospital, 1-396 Kosugi-cho, Nakahara-ku, Kawasaki City, Kanagawa, Japan.
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Abstract

The distribution and abundance of mast cells in nasal polyps, the maxillary sinus mucosa of patients with sinusitis and the turbinate mucosa of allergic rhinitis was microscopically examined using different methods of fixation. In the epithelium of the surface and the ducts of nasal polyps (n = 8), the mean number of mast cells was over 20,000 per mm3 using Mota's fixation and the increase was correlated with the epithelial thickness (P<0.05). On the other hand those of the maxillary sinus mucosa (n = 6) and the nasal turbinate mucosa (n = 7) were less than 6,000 per mm3. In the subepithelial layer or areas deeper than the area with the glands, however, mast cell counts were less than 3,200 per mm3 in all diseases. More than 70–90 per cent of all mast cells in the epithelium of the mucosal surface and the ducts of the polyp, the maxillary sinus mucosa and nasal turbinates were formalin sensitive. Most of the mast cells in the subepithelial and deeper areas were formalin resistant in all diseases.

These results suggest that conditions for mast cell growth differ between polyps and the other diseases, and that the conditions which affect mast cells may contribute to polyp development.

Keywords

Nasal polypsMast cellsEpitheliumSinusitisRhinitis, allergic
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 107 , Issue 5 , May 1993 , pp. 418 - 422
Copyright
Copyright © JLO (1984) Limited 1993

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References

Baumgarten, C., Kunkel, G., Rudolph, R., Staud, R. D., Sperner, I., Gelderblom, H. (1980) Histopathological examinations of nasxsal polyps of different etiology. Archives of Otorhinolaryngology 226: 187–197Google Scholar
Bumsted, R. M., El-Ackad, T., Smith, J. M., Brody, M. J. (1979) Histamine, norepinephrine and serotonin content of nasal polyps. The Laryngoscope 89: 832–843CrossRefGoogle ScholarPubMed
Busutil, A., Chandrachud, H., Kerr, A. I. G., Meudell, G. (1978) Simple nasal polyps and allergic manifestations. Journal of Laryngology and Otology 92: 477–488Google Scholar
Drake-Lee, A. B., McLaughlan, P. (1982) Clinical symptoms, free histamine and IgE in patients with nasal polyposis. International Archives of Allergy and Applied Immunology 69: 268–271CrossRefGoogle ScholarPubMed
Drake-Lee, A. B., McLaughlin, P. (1988) The release of histamine from nasal polyp tissue and peripheral blood when challenged with antihuman IgE, house dust mite extract and mixed grass pollen extract and compared with positive skin tests. Journal of Laryngology and Otology 102: 886–889Google Scholar
Drake-Lee, A. B., Bickerton, R., McLaughlin, P. (1984) Free histamine in nasal polyp fluid. Rhinology 22: 133–138Google Scholar
Drake-Lee, A. B., Chevreton, E., Lowe, D. (1988) The effects of different fixations on the distribution and numbers of mast cells in patients with nasal polyps. Journal of Laryngology and Otology 102: 1099–1101CrossRefGoogle ScholarPubMed
Goto, T., Befus, A. D., Low, R., Bienenstock, J. (1984) Mast cell heterogeneity and hyperplasia in bleomycin-induced pulmonary fibrosis of rats. American Review of Respiratory Disease 130: 797–802Google Scholar
Kaliner, M., Wasserman, S. I., Austen, K. F. (1973) Immunologic release of chemical mediators from human nasal polyp. The New England Journal of Medicine 289: 277–281CrossRefGoogle Scholar
Kawabori, S., Denburg, J. A., Schwarz, L. B., Irani, A. A., Wong, D., Jordana, G., Evans, S., Dolovich, J. (1992) Histochemical and immunohistochemical characteristics of mast cells in nasal polyps. American Journal Respiratory Cell Molecular Biology 6: 37–43CrossRefGoogle ScholarPubMed
Krajina, Z., Zirdum, A. (1987) Histochemical analysis of nasal polyps. Ada Otolaryngologica (Stockholm) 103: 435–440Google ScholarPubMed
Lange, S., Nygren, H., Svennerholm, A. M., Holmgren, J. (1980) Antioxic cholea immunity in mice: influence of antigen deposition on antitoxin–containing cells and protective immunity in different parts of the intestine. Infection and Immunity 28: 17–23CrossRefGoogle Scholar
Ohnishi, M., Ruhno, J., Dolovich, J., Bienenstock, J., Denburg, J. A. (1989) Hematopoietic growth factor production by cultured cells of human nasal polyp epithelium scrapings: kinetics, cell source, and relationship to clinical status. Journal of Allergy and Clinical Immunology S3: 1091–1100Google Scholar
Okuda, M., Otsuka, H. (1977) Basophilic cells in allergic nasal secretions. Archives of Otorhinolaryngology 214: 283–289CrossRefGoogle ScholarPubMed
Otsuka, H., Okuda, M. (1981) Important factors in the nasal manifestation of allergy. Archives of Otorhinolaryngology 233: 227–235Google Scholar
Otsuka, H., Denburg, J., Dolovich, J., Hich, D., Lapp, P., Rajan, R. S., Bienenstock, J., Befus, D. (1985) Heterogeneity of metachromatic cells in human nose: Significance of mucosal mast cells. Journal of Allergy and Clinical Immunology 76: 695–702.CrossRefGoogle ScholarPubMed
Otsuka, H., Dolovich, J., Richardson, M., Bienenstock, J., Denburg, A. (1987) Metachromatic cell progenitors and specific growth anddifferentiation factors in human nasal mucosa and polyps. American Review of Respiratory Disease 136: 710–717CrossRefGoogle Scholar
Salari, H., Borgeat, P., Steffenrud, S., Richard, J., Bedard, P. M., Hebert, J., Pelletier, G. (1986) Immunological and non-immunological release of leukotrienes and histamine from human nasal polyps. Clinical and Experimental Immunology 63: 711–717Google Scholar
Schleimer, R. P., Fox, C. C, Naclerio, R. M. (1985) Role of basophils and mast cells in the pathogenesis of allergic diseases. Journal of Allergy and Clinical Immunology 76: 369–374Google Scholar
Small, P., Frenkiel, S., Black, M. (1981) Multifactorial etiology of nasal polyps. Annals of Allergy 46: 317–320Google Scholar
Takasaka, T., Kaku, Y., Hozawa, K. (1986) Mast cell degranulation in nasal polyps. Ada Otolaryngologica (Stockholm) 430: 39–48Google Scholar
Tos, M., Mogensen, C. (1977) Mucous glands in nasal polyps. Archives of Otolaryngology 103: 407–413Google Scholar