Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-29T06:46:40.086Z Has data issue: false hasContentIssue false
  • English
  • Français

Microenvironment of macula flava in the human vocal fold as a stem cell niche

Published online by Cambridge University Press:  08 June 2016

K Sato ,
S Chitose ,
T Kurita  and
H Umeno
K Sato*
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Japan
S Chitose
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Japan
T Kurita
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Japan
H Umeno
Affiliation:
Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, Japan
*
Address for correspondence: Dr Kiminori Sato, Department of Otolaryngology – Head and Neck Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan Fax: +81 942 37 1200 E-mail: kimisato@oct-net.ne.jp
Get access Rights & Permissions [Opens in a new window]

Abstract

Background:

There is growing evidence that the cells in the maculae flavae are tissue stem cells of the human vocal fold mucosa, and that the maculae flavae are a candidate for a stem cell niche. The role of microenvironment in the maculae flavae of the human vocal fold mucosa was investigated.

Method:

Anterior maculae flavae from six surgical specimens were cultured in a mesenchymal stem cell growth medium or a Dulbecco's modified Eagle's medium.

Results:

Using mesenchymal stem cell growth medium, the subcultured cells formed a colony-forming unit, and cell division reflected asymmetric self-renewal. This indicates that these cells are mesenchymal stem cells or stromal stem cells in the bone marrow. Using Dulbecco's modified Eagle's medium, the subcultured cells showed symmetric cell division without a colony-forming unit.

Conclusion:

A proper microenvironment in the maculae flavae of the human vocal fold mucosa is necessary to be effective as a stem cell niche that maintains the stemness of the contained tissue stem cells.

Keywords

Vocal CordStem CellsStem Cell NicheLarynx
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 130 , Issue 7 , July 2016 , pp. 656 - 661
Copyright
Copyright © JLO (1984) Limited 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Presented at the 136th Annual Meeting of the American Laryngological Association, 22–23 April 2015, Boston, Massachusetts, USA.

References

1Hirano, M, Sato, K. Histological Color Atlas of the Human Larynx. San Diego: Singular Publishing Group, 1993Google Scholar
2Sato, K, Hirano, M, Nakashima, T. Stellate cells in the human vocal fold. Ann Otol Rhinol Laryngol 2001;110:319–25Google Scholar
3Sato, K, Hirano, M, Nakashima, T. Vitamin A-storing stellate cells in the human vocal fold. Acta Otolaryngol 2003;123:106–10Google Scholar
4Sato, K, Umeno, H, Nakashima, T. Functional histology of the macula flava in the human vocal fold. Part 1: Its roles in the adult vocal fold. Folia Phoniatr Logop 2010;62:178–84Google Scholar
5Sato, K, Hirano, M, Nakashima, T. Fine structure of the human newborn and infant vocal fold mucosae. Ann Otol Rhinol Laryngol 2001;110:417–24CrossRefGoogle ScholarPubMed
6Sato, K, Nakashima, T. Vitamine A-storing stellate cells in the human newborn vocal fold. Ann Otol Rhinol Laryngol 2005;114:517–24CrossRefGoogle ScholarPubMed
7Sato, K, Umeno, H, Nakashima, T. Functional histology of the macula flava in the human vocal fold. Part 2: Its roles in the growth and development of the vocal fold. Folia Phoniatr Logop 2010;62:263–70Google Scholar
8Sato, K, Hirano, M, Nakashima, T. Age-related changes in vitamine A-storing stellate cells of human vocal fold. Ann Otol Rhinol Laryngol 2004;113:108–12CrossRefGoogle Scholar
9Sato, K, Umeno, H, Nakashima, T. Vocal fold stellate cells in the human macula flava and the diffuse stellate cell system. Ann Otol Rhinol Laryngol 2012;121:51–6CrossRefGoogle ScholarPubMed
10Sato, K, Umeno, T, Nakashima, T. Vocal fold stem cells and their niche in the human vocal fold. Ann Otol Rhinol Laryngol 2012;121:798–803Google Scholar
11Xie, T, Li, L. Stem cell niche: structure and function. Annu Rev Cell Dev Biol 2005;21:605–31Google Scholar
12Nilsson, SK, Haylock, DN. The role of hyaluronic acid in hemopoietic stem cell biology. Regen Med 2006;1:437–45Google Scholar
13Preston, M, Sherman, LS. Neural stem cell niches: roles for the hyaluronan-based extracellular matrix. Front Biosci (Schol Ed) 2011;3:1165–79CrossRefGoogle ScholarPubMed
14Toole, BP. Proteoglycans and hyaluronan in morphogenesis and differentiation. In: Hay, E, ed. Cell Biology of Extracellular Matrix, 2nd edn.New York: Plenum Press, 1991;305–41Google Scholar
15Becker, WM, Kleinsmith, LJ, Hardin, J. The cell cycle, DNA replication, and mitosis. In: The World of the Cell, 6th edn.San Francisco: Pearson Education, publishing as Benjamin Cummings, 2006;554–71Google Scholar
16Kurita, T, Sato, K, Chitose, S, Fukahori, M, Sueyoshi, S, Umeno, H. Origin of vocal fold stellate cells in the human macula flava. Ann Otol Rhinol Laryngol 2015;124:698–705Google Scholar
17Friedenstein, AJ, Deriglasova, UF, Kulagina, NN, Panasuk, AF, Rudakowa, SF, Luria, EA et al. Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method. Exp Hematol 1974;2:83–92Google ScholarPubMed
18Pittenger, MF, Mackay, AM, Beck, SC, Jaiswal, RK, Douglas, R, Mosca, JD et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143–7CrossRefGoogle ScholarPubMed
19Thomson, JA, Itskovitz-Eldor, J, Shapiro, SS, Waknitz, MA, Swiergiel, JJ, Marshall, VS et al. Embryonic stem cell lines derived from human blastocysts. Science 1998;282:1145–7Google Scholar
20Takahashi, K, Tanabe, K, Ohnuki, M, Narita, M, Ichisaka, T, Tomoda, K et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007;131:861–72Google Scholar
21Suzuki, A, Zheng, YW, Kondo, R, Kusakabe, M, Takada, Y, Fukao, K et al. Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver. Hepatology 2000;32:1230–9Google Scholar
22Osafune, K, Takasato, M, Kispert, A, Asashima, M, Nishinakamura, R. Identification of multipotent progenitors in the embryonic mouse kidney by a novel colony-forming assay. Development 2006;133:151–61Google Scholar
23Deasy, BM. Asymmetric behavior in stem cells. In: Rajasekhar, VK, Vemuri, MC, ed. Regulatory Networks in Stem Cells. New York: Humana Press, 2009;13–25Google Scholar