Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-29T12:55:20.810Z Has data issue: false hasContentIssue false
  • English
  • Français

p53 and bcl-2 expression and argyrophilic nucleolar organizer regions in patients with malignant maxillary sinus tumours

Published online by Cambridge University Press:  29 June 2007

Torahiko Nakashima ,
Tadashi Nakashima ,
Muneyuki Masuda  and
Sohtaro Komiyama
Torahiko Nakashima*
Affiliation:
Departments of Otorhinolaryngology, Faculty of Medicine, Kyushu, Japan.
Tadashi Nakashima
Affiliation:
Kyushu University and the National Kyushu Cancer Center, Kyushu, Japan.
Muneyuki Masuda
Affiliation:
Departments of Otorhinolaryngology, Faculty of Medicine, Kyushu, Japan.
Sohtaro Komiyama
Affiliation:
Departments of Otorhinolaryngology, Faculty of Medicine, Kyushu, Japan.
*
Address for correspondence: Torahiko Nakashima, 3-1-1 Maidashi Higashi-ku, Fukuoka City, Japan 812. Fax: 81-92-651-0975
Get access Rights & Permissions [Opens in a new window]

Abstract

To investigate the relation between the clinicopathological findings, and the survival of patients with malignant tumours of the maxillary sinus, and the tumour's biological parameters, we examined the expression of p53 and bcl-2 and the number of silver-stainable nucleolar organizer regions (AgNORs) in 23 paraffin-embedded specimens from primary tumours. Fifteen of 23 (65 per cent) maxillary tumours expressed p53 and two of 23 (nine per cent) tumours expressed the bcl-2 gene product. The mean number of AgNORs per nucleus was 5.2 ± 2.4. There was no relationship between the expression of p53 or bcl-2 and the patient's clinical course. In contrast, there was a statistically significant difference in the survival of patients with a high (>6) number of AgNORs and patients with a low (<6) number of AgNORs. These data suggest that the number of AgNORs may be useful in evaluating the prognosis of patients with malignant maxillary sinus tumours.

Keywords

Maxillary sinus neoplasmsProteinp 53bcl-2; Nucleolus organizer region
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 111 , Issue 1 , January 1997 , pp. 38 - 42
Copyright
Copyright © JLO (1984) Limited 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barry, M. A., Behnke, C. A., Eastman, A. (1990) Activation of programmed cell death (apoptosis) by cisplatin, other anticancer drugs, toxins and hyperthermia. Biochemical Pharmacology 40: 2352–2362.CrossRefGoogle ScholarPubMed
Fallowfield, M. E., Cook, M. G. (1989) The value of nucleolar organizer region staining in the differential diagnosis of borderline melanocytic lesions. Histopathology 14: 299–304.CrossRefGoogle ScholarPubMed
Field, J. K., Pavelic, Z. P., Spandidos, D. A., Stambrook, P. J., Jones, A. S., Gluckman, J. L. (1993) The role of the p53 tumour suppressor gene in squamous cell carcinoma of the head and neck. Archives of Otolaryngology, Head and Neck Surgery 119(10): 1118–1122.CrossRefGoogle ScholarPubMed
Hermanek, P., Sobin, L. H. (1992) UICC TNM Classification of malignant tumours. 4th Edition 2nd rev. Springer-Verlag: Berlin.Google Scholar
Hockenbery, D. M., Zutter, M., Hickey, W., Nahm, M., Korsmeyer, S. J. (1990) bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature (Lond.) 348: 334–336.CrossRefGoogle ScholarPubMed
Komiyama, S., Hiroto, I., Ryu, S., Nakashima, T., Kuwano, M., Endo, H. (1978) Synergistic combination therapy of 5-fluorouracil, vitamin A, and cobalt-60 upon head and neck tumours. Oncology 35: 253–257.CrossRefGoogle Scholar
Lane, D. P., Benchimol, S. (1990) p53: Oncogene or antioncogene? Genes and Development 4: 1–8.CrossRefGoogle ScholarPubMed
Leek, R. D., Kaklamanis, L., Pezzella, F., Gatter, K. C., Harris, A. L. (1994) bcl-2 in normal human breast and carcinoma, association with oestrogen receptor-positive, epidermal growth factor receptor-negative tumours and in situ cancer. British Journal of Cancer 69(1): 135–139.CrossRefGoogle ScholarPubMed
Lowe, S. W., Ruley, H. E., Jacks, T., Housman, D. E. (1993) p53-dependent apoptosis modulates the cytotoxicity of anticancer agents. Cell 74: 957–967.CrossRefGoogle ScholarPubMed
Miyaguchi, M., Kawahara, T., Sakai, S., Kuwabara, H., Sakamoto, H. (1994) A correlation of argyrophilic nucleolar organizer regions with prognosis in patients with maxillary sinus squamous cell carcinoma. European Archives of Otorhinolaryngology 251 4: 229–232.CrossRefGoogle ScholarPubMed
Morgan, D. W., Crocker, J., Watts, S., Shenoi, P. M. (1988) Salivary gland tumours studied by means of the AgNOR technique. Histopathology 13: 553–559.CrossRefGoogle ScholarPubMed
Nakashima, T., Yano, G., Masuda, A., Uemura, T., Morita, M. (1994) Argyrophilic nucleolar organizer regions as a prognostic indicator of laryngeal carcinomas. European Archives of Otorhinolaryngology 251 (Suppl. 1): 76–79.CrossRefGoogle ScholarPubMed
Pezzella, F., Turley, H., Kuzu, I., Tungekar, M. F., Dunnill, M. S., Pierce, C. B., Harris, A., Gatter, K. C., Mason, D. Y. (1994) bcl-2 protein in non-small-cell lung carcinoma. New England Journal of Medicine 329: 690–694.CrossRefGoogle Scholar
Pilotti, S., Collini, P., Rilke, F., Cattoretti, G., Del Bo, R., Pierotti, M. (1994) bcl-2 protein expression in carcinomas originating from the follicular epithelium of the thyroid gland. Journal of Pathology 172: 337–342.CrossRefGoogle ScholarPubMed
Ploton, D., Menegar, M., Jeannesson, P., Himber, G., Pigeon, F., Adnett, J. (1986) Improvement in the staining and in the visualisation of argyrophilic proteins of the nucleolar organizer region at the optic level. Histochemical Journal 18: 5–14.CrossRefGoogle ScholarPubMed
Shchepotin, I. B., Soldatenkov, V., Buras, R. R., Nauta, R. J., Shabahang, M.; Evans, S. R. (1994) Apoptosis of human primary and metastatic colon adenocarcinoma cell lines in vitro induced by 5-fluorouracil, verapamil and hyperthermia. Anticancer Research 14: 1027–1032.Google ScholarPubMed
Shin, D. M., Kim, J., Ro, J. Y., Hittleman, J., Roth, J. A., Hong, W. K., Hittelman, W. N. (1994) Activation of p53 gene expression in premalignant lesion during head and neck tumorigenesis. Cancer Research 54: 321–326.Google ScholarPubMed
Tsujimoto, Y., Finger, L. R., Yunis, J., Nowell, P. C., Croce, C. M. (1984) Cloning of the chromosome breakpoint of neoplastic B cells with the t(14;18) chromosome translocation. Science 226: 1097–1099.CrossRefGoogle Scholar
Yang, P., Huang, G. S., Zhu, X. S. (1990) Role of nucleolar organizer regions in differentiating malignant from benign tumours of the colon. Journal of Clinical Pathology 43: 235–238.CrossRefGoogle ScholarPubMed
Vaux, D. L., Cory, S., Adams, J. (1988) bcl-2 gene promotes haematopoietic cell survival and cooperates with c-myc to immortalize pre-B cells. Nature 335: 440–442.CrossRefGoogle ScholarPubMed
Zhao, M., Zhang, N. X., Economou, M., Blaha, I., Laissue, J. A., Zimmermann, A. (1994) Immunohistochemical detection of bcl-2 protein in liver lesions: bcl-2 protein is expressed in hepatocellular carcinomas but not in liver cell dysplasia. Histopathology 25(3): 237–245.CrossRefGoogle ScholarPubMed