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Primary study in experimental antiangiogenic therapy of nasopharyngeal carcinoma with AGM-1470 (TNP-470)

Published online by Cambridge University Press:  29 June 2007

Chao-nan Qian ,
Hua-Qing Min ,
Han-liang Lin ,
Ming-Huang Hong  and
Yan-li Ye
Chao-nan Qian*
Affiliation:
Department of Nasopharyngeal Carcinoma, Tumor Hospital, Cancer Center, Sun Yat-sen University of Medical Sciences, (SUMS), Guangzhou, P.R. of China.
Hua-Qing Min
Affiliation:
Department of Nasopharyngeal Carcinoma, Tumor Hospital, Cancer Center, Sun Yat-sen University of Medical Sciences, (SUMS), Guangzhou, P.R. of China.
Han-liang Lin
Affiliation:
Department of Pathology, Tumor Hospital, Cancer Center, Sun Yat-sen University of Medical Sciences, (SUMS), Guangzhou, P.R. of China.
Ming-Huang Hong
Affiliation:
Department of Nasopharyngeal Carcinoma, Tumor Hospital, Cancer Center, Sun Yat-sen University of Medical Sciences, (SUMS), Guangzhou, P.R. of China.
Yan-li Ye
Affiliation:
Department of Animal Laboratory of the Cancer Center, Tumor Hospital, Cancer Center, Sun Yat-sen University of Medical Sciences, (SUMS), Guangzhou, P.R. of China.
*
Address for correspondence: Chao-nan Qian, M.D., Department of Nasopharyngeal Carcinoma, Tumor Hospital, Sun Yat-sen University of Medical Sciences, 651 Dongfeng East Road, Guangzhou 510060, P. R. of China. Fax: +86-20-87754506 E-mail: gzqcnan@publicl.guangzhou.gd.cn
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Abstract

Objective

To evaluate the efficacy of the angiogenesis inhibitor AGM-1470 for the experimental treatment of nasopharyngeal carcinoma (NPC).

Methods

A NPC human tumour model was built by tumour-bearing nude mice using the NPC cell line CNE-2. Twenty-one BALB/c nude mice bearing CNE-2 xenografts were randomized into a treatment group and a control group. In the treatment group, AGM-1470 was injected 30 mg/kg subcutaneously every other day; while the vehicle (three per cent ethanol solution in 0.9 per cent saline) was given to the mice in control group. Tumour volumes and animal weights were measured every third day. Autopsy was performed after 18 days of treatment. The tumour tissue as well as the murine tissues of heart, kidney, and liver in each mouse were removed for formalin fixation and routine HE staining. Pathological evaluation was performed in these tissues.

Results

There was a significant difference in tumour volume between the two groups at day 9 of treatment and this increased thereafter. At day 15 of treatment, the tumour volume was 4251 ± 559 mm3 (n = 10) in the control group versus 3122 ± 967 mm3 (n = 11) in the AGM-1470 treated group (p = 0.004); and T:C ratio (mean tumour volume of treated/mean tumour volume of control) was 0.73, resulting in a 27 per cent decrease in tumour growth. Central necrosis and consequential shrinkage of tumours occurred in both groups at the end of experiment. Physical toxicity and histological toxicity of heart, liver, and kidney did not result from AGM-1470 therapy.

Conclusions

AGM-1470 suppresses the growth of the human NPC cell line CNE-2. Treatment by AGM-1470 has no physical nor histological toxicity. Angiogenesis inhibitors may be effective in the treatment of the local lesion of NPC.

Keywords

Nasopharyngeal neoplasmsCarcinomaNeovasculanzation inhibition, treatment
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 112 , Issue 9 , September 1998 , pp. 849 - 853
Copyright
Copyright © JLO (1984) Limited 1998

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