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Structural changes and immunohistochemical localisation of epidermal growth factor receptor in the true vocal fold of female albino rats administered anabolic, androgenic steroids, and effects of anti-androgen therapy

Published online by Cambridge University Press:  13 May 2011

H E Amer ,
S A Asker  and
S A Mazroa
H E Amer*
Affiliation:
Department of Otorhinolaryngology, Faculty of Medicine, Mansoura University, Egypt
S A Asker
Affiliation:
Department of Histology and Cell Biology, Faculty of Medicine, Mansoura University, Egypt
S A Mazroa
Affiliation:
Department of Histology and Cell Biology, Faculty of Medicine, Mansoura University, Egypt
*
Address for correspondence: Dr Hazem Emam Amer, Otorhinolaryngology Department, Mansoura University Hospital, Mansoura 35516, Egypt E-mail: hazememam47@yahoo.com
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Abstract

Background:

Anabolic steroid abuse by women is associated with a number of adverse effects, including laryngeal changes. The epidermal growth factor receptor is related to regulation of the cell life cycle. This study aimed to investigate the structural changes and immunohistochemical localisation of epidermal growth factor receptor in rat vocal folds following anabolic steroid administration, and also to assess the effect of anti-androgens.

Material and methods:

Thirty-two adult female albino rats were divided into: group I (controls), group II (receiving anabolic steroids for two months) or group III (receiving anabolic steroids plus anti-androgen for two months).

Results:

Group II rat true vocal folds showed thicker epithelial layers with many mitotic figures, thicker lamina propria and thicker muscle fibres; epithelial cells were also immunohistochemically positive for epidermal growth factor receptor. Group III rats showed similar changes, but thin muscle fibres and extravasated red blood cells within the lamina propria.

Conclusion:

Anabolic steroids caused structural and immunohistochemical changes within the female rat true vocal fold. Co-administration of anti-androgens did not prevent these changes, suggesting that anti-androgens have a limited role in the management of such changes in humans.

Keywords

Receptor, Epidermal Growth FactorVocal CordsAnabolic AgentsFlutamideImmunohistochemistryModel, AnimalRats
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 125 , Issue 8 , August 2011 , pp. 829 - 836
Copyright
Copyright © JLO (1984) Limited 2011

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References

1Penatti, CA, Porter, DM, Henderson, LP. Chronic exposure to anabolic androgenic steroids alters neuronal function in the mammalian forebrain via androgen receptor- and estrogen receptor-mediated mechanisms. J Neurosci 2009;29:12484–96CrossRefGoogle ScholarPubMed
2Hartgens, F, Kuipers, H. Effects of androgenic-anabolic steroids in athletes. Sports Med 2004;34:513–54CrossRefGoogle ScholarPubMed
3Clark, AS, Kelton, MC, Whitney, AC. Chronic administration of anabolic steroids disrupts pubertal onset and estrous cyclicity in rats. Biol Reprod 2003;68:465–71CrossRefGoogle ScholarPubMed
4Damrose, EJ. Quantifying the impact of androgen therapy on the female larynx. Auris Nasus Larynx 2008;36:110–12CrossRefGoogle Scholar
5Ray, S, Masood, A, Pickles, J, Moumoulidis, I. Severe laryngitis following chronic anabolic steroid abuse. J Laryngol Otol 2008;122:230–2CrossRefGoogle ScholarPubMed
6Schultz, GS, White, M, Mitchell, R, Brown, G, Lynch, J, Twardzik, DR et al. Epithelial wound healing enhanced by transforming growth factor-alpha and vaccinia growth factor. Science 1987;235:350–2CrossRefGoogle ScholarPubMed
7Steidler, NE, Reade, PC. Histomorphological effects of epidermal growth factor on skin and oral mucosa in neonatal mice. Arch Oral Biol 1980;25:37–43CrossRefGoogle ScholarPubMed
8Carpenter, G. Receptor tyrosine kinase substrates: SRC homology domains and signal transduction. FASEB J 1992;6:3283–9CrossRefGoogle ScholarPubMed
9Langner, C, Ratschek, M, Rehak, P, Schips, L, Zigeuner, R. Are heterogenous results of EGFR immunoreactivity in renal cell carcinoma related to non-standardised criteria for staining evaluation? J Clin Pathol 2004;57:773–5CrossRefGoogle ScholarPubMed
10Jost, M, Kari, C, Rodeck, U. The EGF receptor – an essential regulator of multiple epidermal functions. Eur J Dermatol 2000;10:505–10Google ScholarPubMed
11De Leo, V, Lanzetta, D, D'Antona, D, la Marca, A, Morgante, G. Hormonal effects of flutamide in young women with polycystic ovary syndrome. J Clin Endocrinol Metab 1998;83:99–102CrossRefGoogle ScholarPubMed
12Takahashi, M, Tatsugi, Y, Kohno, T. Endocrinological and pathological effects of anabolic-androgenic steroid in male rats. Endocr J 2004;51:425–34CrossRefGoogle ScholarPubMed
13Whitney, AC, Clark, AS. Effects of acute stanozolol treatment on puberty in female rats. Biol Reprod 2001;64:1460–5CrossRefGoogle ScholarPubMed
14Chen, H, Brahmbhatt, S, Gupta, A, Sharma, A. Duration of streptozotocin-induced diabetes differentially affects p38-mitogen-activated protein kinase (MAPK) phosphorylation in renal and vascular dysfunction. Cardiovasc Diabetol 2005;4:3–11CrossRefGoogle ScholarPubMed
15Stevens, A, Wilson, I. The haematoxylin and eosin. In: Theory and Practice of Histological Techniques (eds. Bancroft, J, Stevens, A, Turner, D). 4th edn., Churchill Livingstone, New York, 1996;99–112Google Scholar
16Luna, L. Manual of Histologic Staining Methods of the Armed Force Institute of Pathology. New York: McGraw-Hill, 1968;72–99Google Scholar
17Altunbas, K, Ozfiliz, N, Yagci, A, Zik, B. Immunohistochemical localization of epidermal growth factor receptor in testes of mice with experimentally induced diabetes. Bull Vet Inst Pulawy 2006;50:405–8Google Scholar
18Gesualdo, L, Di Paolo, S, Calabró, A, Milani, S, Maiorano, E, Ranieri, E et al. Expression of epidermal growth factor and its receptor in normal and diseased human kidney: an immunohistochemical and in situ hybridization study. Kidney Int 1996;49:656–65CrossRefGoogle ScholarPubMed
19Schoonjans, F, Zalata, A, Depuydt, C, Comhaire, F. MedCalc: a new computer program for medical statistics. Comput Methods Programs Biomed 1995;48:257–62CrossRefGoogle ScholarPubMed
20Pedroso, JE, Brasil, OC, Martins, JR, Nader, HB, Simões Mde, J. Analysis of hyaluronic acid concentration in rat vocal folds during estral and gravidic puerperal cycles. Braz J Otorhinolaryngol 2009;75:654–9CrossRefGoogle ScholarPubMed
21Tateya, T, Sohn, JH, Tateya, I, Bless, DM. Histologic characterization of rat vocal fold scarring. Ann Otol Rhinol Laryngol 2005;114:183–92CrossRefGoogle ScholarPubMed
22Lewis, DJ, Prentice, DE. The ultrastructure of rat laryngeal epithelia. J Anat 1980;130:617–32Google ScholarPubMed
23Hirano, M. Structure of the vocal fold in normal and disease states: anatomical and physical studies. ASHA Rep 1981;11:11–30Google Scholar
24Boyd, SK, Wissing, KD, Heinsz, JE, Prins, GS. Androgen receptors and sexual dimorphisms in the larynx of the bullfrog. Gen Comp Endocrinol 1999;113:59–68CrossRefGoogle ScholarPubMed
25Talaat, M, Talaat, AM, Kelada, I, Angelo, A, Elwany, S, Thabet, H. Histologic and histochemical study of effects of anabolic steroids on the female larynx. Ann Otol Rhinol Laryngol 1996;96:468–71CrossRefGoogle Scholar
26Tammi, R, Santti, R. Morphometric analysis of human epidermis treated with testosterone and dehydroepiandrosterone in organ culture. Arch Dermatol Res 1989;281:417–23CrossRefGoogle ScholarPubMed
27Rocha, FL, Carmo, EC, Roque, FR, Hashimoto, NY, Rossoni, LV, Frimm, C et al. Anabolic steroids induce cardiac renin-angiotensin system and impair the beneficial effects of aerobic training in rats. Am J Physiol Heart Circ Physiol 2007;293(6):H3575–83CrossRefGoogle ScholarPubMed
28Andrade, TU, Santos, MC, Busato, VC, Medeiros, AR, Abreu, GR, Moysés, MR et al. Higher physiological doses of nandrolone decanoate do not influence the Bezold-Jarish reflex control of bradycardia. Arch Med Res 2008;39:27–32CrossRefGoogle Scholar
29Montisci, R, Cecchetto, G, Ruscazio, M, Snenghi, R, Portale, A, Viel, G et al. Early myocardial dysfunction after chronic use of anabolic androgenic steroids: combined pulsed-wave tissue Doppler imaging and ultrasonic integrated backscatter cyclic variations analysis. J Am Soc Echocardiogr 2010;23:516–22CrossRefGoogle ScholarPubMed
30Byyny, RL, Orth, DN, Cohen, S. Radioimmunoassay of epidermal growth factor. Endocrinology 1972;90:1261–6CrossRefGoogle ScholarPubMed
31Miyaguchi, M, Olofsson, J, Hellquist, HB. Expression of epidermal growth factor receptor in laryngeal dysplasia and carcinoma. Acta Otolaryngol 1990;110:309–13CrossRefGoogle ScholarPubMed
32Cook, PW, Pittelkow, MR, Shipley, GD. Growth factor-independent proliferation of normal human neonatal keratinocytes: production of autocrine- and paracrine-acting mitogenic factors. J Cell Physiol 1991;146:277–89CrossRefGoogle ScholarPubMed
33Roberts, ML. Testosterone-induced accumulation of epidermal growth factor in the submandibular salivary glands of mice, assessed by radioimmunoassay. Biochem Pharmacol 1974;23:3305–8CrossRefGoogle ScholarPubMed
34Krecicki, T, Jelen, M, Zalesska-Krecicka, M, Rak, J, Szkudlarek, T, Jelen-Krzeszewska, J. Epidermal growth factor receptor (EGFR), proliferating cell nuclear antigen (PCNA) and Ki-67 antigen in laryngeal epithelial lesions. Oral Oncol 1999;35:180–6CrossRefGoogle ScholarPubMed
35Loewit, K, Hussl, B, Richter, E, Schwartz, S. Anti-androgen therapy in pachydermia of the female larynx – a new therapeutic possibility. Arch Otorhinolaryngol 1977;215:75–9CrossRefGoogle ScholarPubMed
36Sassoon, D, Kelley, DB. The sexually dimorphic larynx of Xenopus laevis: development and androgen regulation. Am J Anat 1986;177:457–72CrossRefGoogle ScholarPubMed
37Iliescu, R, Campos, LA, Schlegel, WP, Morano, I, Baltatu, O, Bader, M. Androgen receptor independent cardiovascular action of the antiandrogen flutamide. J Mol Med 2003;81:420–7CrossRefGoogle ScholarPubMed
38Wright, CL, Cacala, S. Enterocolic lymphocytic phlebitis with lymphocytic colitis, lymphocytic appendicitis, and lymphocytic enteritis. Am J Surg Pathol 2004;28:542–7CrossRefGoogle ScholarPubMed