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Subcutaneous tissue reaction to synthetic auditory ossicle (Apaceram®) in rats

Published online by Cambridge University Press:  29 June 2007

Peng-Cheng Cui ,
Katsuichiro Ohsaki ,
Kunio Ii ,
Satoru Tenshin  and
Terushige Kawata
Peng-Cheng Cui
Affiliation:
Division of Clinical Otology, School of Dentistry, The University of Tokushima, Tokushima 770, Japan.
Katsuichiro Ohsaki*
Affiliation:
Division of Clinical Otology, School of Dentistry, The University of Tokushima, Tokushima 770, Japan.
Kunio Ii
Affiliation:
Department of Pathology, School of Medicine, The University of Tokushima, Tokushima 770, Japan.
Satoru Tenshin
Affiliation:
Department of Orthodontics, School of Dentistry, The University of Tokushima, Tokushima 770, Japan.
Terushige Kawata
Affiliation:
Department of Orthodontics, School of Dentistry, The University of Tokushima, Tokushima 770, Japan.
*
Address for correspondence: Katsuichiro Ohsaki, M.D., Division of Clinical Otology, University Hospital, The University of Tokushima, School of Medicine, Tokushima 770, Japan. Fax: (0886) 33-7208.
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Abstract

A study was carried out in order to obtain further information about the soft tissue response to thin Apaceram® discs of dense hydroxyapatite (HA) implanted in rats for various periods of time between one day and 10 months. The Apaceram® discs were implanted subcutaneously into the interscapular region of 33 rats. A sham operation was performed on eight rats used as controls. Decalcified histological sections stained with haematoxylin and eosin and Mallory's azan were examined and the different cell types found around the implants were counted. It was found that an acute inflammatory reaction occurred after one day and disappeared at about two weeks after implantation. In the test groups, macrophages and lymphocytes disappeared about one week later, and no inflammatory reaction was observed from one to three months. However, a tissue reaction occurred at six months with the appearance of macrophages and lymphocytes, and decreased gradually at 10 months. Meanwhile, a few foreign body giant cells at the Apaceram®-tissue interface and a thick layer of fibrous connective tissue around the Apaceram® disc were observed at 10 months. No osteogenesis was observed in any specimen. The results obtained so far suggest that Apaceram® is still a useful material for reconstructive surgery, despite the possible appearance of a slight macrophage reaction at six months.

Keywords

HydroxyapatitesHistologyCell countBiocompatible materialsRats
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 109 , Issue 1 , January 1995 , pp. 14 - 18
Copyright
Copyright © JLO (1984) Limited 1995

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Footnotes

This paper was presented at the West Japan International Symposium on Ear Surgery in Miyazaki, organized by Miyazaki Medical College, Miyazaki, Japan, on 14 May, 1994.

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