Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-27T13:34:15.411Z Has data issue: false hasContentIssue false
  • English
  • Français

The use of fluorescence lifetime technology in benign and malignant thyroid tissues

Published online by Cambridge University Press:  10 July 2019

G Nakache ,
G Yahav ,
G H Siloni ,
I Barshack ,
E Alon ,
M Wolf  and
D Fixler
G Nakache*
Affiliation:
Department of Otolaryngology Head and Neck Surgery, Rabin Medical Center, Petach Tikva, Israel
G Yahav
Affiliation:
Faculty of Engineering, Bar Ilan University, Raman Gan, Israel
G H Siloni
Affiliation:
Sheba Tissue Bank, Sheba Medical Center, Ramat Gan, Israel
I Barshack
Affiliation:
Department of Pathology, Sheba Medical Center, Ramat Gan, Israel
E Alon
Affiliation:
Department of Otolaryngology Head and Neck Surgery, Sheba Medical Center, Ramat Gan, Israel
M Wolf
Affiliation:
Department of Otolaryngology Head and Neck Surgery, Sheba Medical Center, Ramat Gan, Israel
D Fixler
Affiliation:
Faculty of Engineering, Bar Ilan University, Raman Gan, Israel
*
Author for correspondence: Dr Gabriel Nakache, Department of Otolaryngology Head and Neck Surgery, Rabin Medical Center, Petach Tikva, Israel E-mail: nakachega@gmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective

To explore the use of fluorescence lifetime imaging microscopy in thyroid tissues, and to investigate how different thyroid lesions affect fluorescence lifetime.

Method

Fluorescence lifetime measurements were taken of fresh frozen thyroid surgical specimens stained with fluorescein isothiocyanate tagged anti-thyroglobulin monoclonal antibodies.

Results

The mean fluorescence lifetime measurements in 12 patients – 3 with multinodular goitre, 4 with follicular adenoma, 4 with papillary thyroid carcinoma and 1 with follicular carcinoma – were 3.16 ns (range, 2.66–3.52 ns), 3.75 ns (range, 2.99–4.57 ns), 2.97 ns (range, 2.57–3.21 ns) and 3.61 ns, respectively. The fluorescence lifetime of follicular adenoma patients was higher than that of papillary thyroid carcinoma patients by 26 per cent (p = 0.058). The fluorescence lifetime in the follicular carcinoma patient was similar to the follicular adenoma group, but higher than in the papillary thyroid carcinoma group by 22 per cent (p = 0.01).

Conclusion

Fluorescence lifetime measurements varied in different thyroid pathologies, possibly because of tissue-scale structural influences.

Keywords

Thyroid NoduleFluorescence Microscopy
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 133 , Issue 8 , August 2019 , pp. 696 - 699
Copyright
Copyright © JLO (1984) Limited, 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Dr G Nakache takes responsibility for the integrity of the content of the paper

References

1Haugen, BR, Alexander, EK, Bible, KC, Doherty, GM, Mandel, SJ, Nikiforov, YE et al. 2015 American Thyroid Association Management Guidelines for Adult Patients with Thyroid Nodules and Differentiated Thyroid Cancer: The American Thyroid Association Guidelines Task Force on Thyroid Nodules and Differentiated Thyroid Cancer. Thyroid 2016;26:1–133Google Scholar
2Marqusee, E, Benson, CB, Frates, MC, Doubilet, PM, Larsen, PR, Cibas, ES et al. Usefulness of ultrasonography in the management of nodular thyroid disease. Ann Intern Med 2000;133:696–700Google Scholar
3Brito, JP, Gionfriddo, MR, Al, NA, Boehmer, KR, Leppin, AL, Reading, C et al. The accuracy of thyroid nodule ultrasound to predict thyroid cancer: systematic review and meta-analysis. J Clin Endocrinol Metab 2014;99:1253–63Google Scholar
4Danese, D, Sciacchitano, S, Farsetti, A, Andreoli, M, Pontecorvi, A. Diagnostic accuracy of conventional versus sonography-guided fine-needle aspiration biopsy of thyroid nodules. Thyroid 1998;8:15–21Google Scholar
5Cibas, ES, Ali, SZ. The Bethesda System for Reporting Thyroid Cytopathology. Am J Clin Pathol 2009;132:658–65Google Scholar
6Bongiovanni, M, Spitale, A, Faquin, WC, Mazzucchelli, L, Baloch, ZW. The Bethesda System for Reporting Thyroid Cytopathology: a meta-analysis. Acta Cytol 2012;56:333–9Google Scholar
7Ohori, NP, Schoedel, KE. Variability in the atypia of undetermined significance/follicular lesion of undetermined significance diagnosis in the Bethesda System for Reporting Thyroid Cytopathology: sources and recommendations. Acta Cytol 2011;55:492–8Google Scholar
8Braga, M, Cavalcanti, TC, Collaco, LM, Graf, H. Efficacy of ultrasound-guided fine-needle aspiration biopsy in the diagnosis of complex thyroid nodules. J Clin Endocrinol Metab 2001;86:4089–91Google Scholar
9Alexander, EK, Heering, JP, Benson, CB, Frates, MC, Doubilet, PM, Cibas, ES et al. Assessment of nondiagnostic ultrasound-guided fine needle aspirations of thyroid nodules. J Clin Endocrinol Metab 2002;87:4924–7Google Scholar
10Kroll, TG, Sarraf, P, Pecciarini, L, Chen, CJ, Mueller, E, Spiegelman, BM et al. PAX8-PPAR gamma1 fusion oncogene in human thyroid carcinoma. Science 2000;289:1357–60Google Scholar
11Umbricht, CB, Saji, M, Westra, WH, Udelsman, R, Zeiger, MA, Sukumar, S. Telomerase activity: a marker to distinguish follicular thyroid adenoma from carcinoma. Cancer Res 1997;57:2144–7Google Scholar
12Nikiforov, YE. Role of molecular markers in thyroid nodule management: then and now. Endocr Pract 2017;23:979–88Google Scholar
13Febbo, PG, Ladanyi, M, Aldape, KD, De Marzo, AM, Hammond, ME, Hayes, DF et al. NCCN Task Force report: evaluating the clinical utility of tumor markers in oncology. J Natl Compr Canc Netw 2011;9(suppl 5):S1–32Google Scholar
14Xing, M, Haugen, BR, Schlumberger, M. Progress in molecular-based management of differentiated thyroid cancer. Lancet 2013;381:1058–69Google Scholar
15Ferris, RL, Baloch, Z, Bernet, V, Chen, A, Fahey, TJ 3rd, Ganly, I et al. ; American Thyroid Association Surgical Affairs Committee. American Thyroid Association Statement on Surgical Application of Molecular Profiling for Thyroid Nodules: current impact on perioperative decision making. Thyroid 2015;25:760–8Google Scholar
16Chang, C, Sud, D, Mycek, MA. Fluorescence lifetime imaging microscopy. Methods Cell Biol 2007;81:495–524Google Scholar
17Bastiaens, PI, Squire, A. Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell. Trends Cell Biol 1999;9:48–52Google Scholar
18Fixler, D, Tirosh, R, Shainberg, A, Deutsch, M. Cytoplasmic changes in cardiac cells during a contraction cycle detected by fluorescence polarization. J Fluoresc 2001;11:89–100Google Scholar
19Berezin, MY, Achilefu, S. Fluorescence lifetime measurements and biological imaging. Chem Rev 2010;110:2641–84Google Scholar
20Colasanti, A, Kisslinger, A, Fabbrocini, G, Liuzzi, R, Quarto, M, Riccio, P et al. MS-2 fibrosarcoma characterization by laser induced autofluorescence. Lasers Surg Med 2000;26:441–8Google Scholar
21Yahav, G, Hirshberg, A, Salomon, O, Amariglio, N, Trakhtenbrot, L, Fixler, D. Fluorescence lifetime imaging of DAPI-stained nuclei as a novel diagnostic tool for the detection and classification of B-cell chronic lymphocytic leukemia. Cytometry A 2016;89:644–52Google Scholar
22Pradhan, A, Pal, P, Durocher, G, Villeneuve, L, Balassy, A, Babai, F et al. Steady state and time-resolved fluorescence properties of metastatic and non-metastatic malignant cells from different species. J Photochem Photobiol B 1995;31:101–12Google Scholar
23Zahavi, T, Yahav, G, Shimshon, Y, Gershanov, S, Kaduri, L, Sonnenblick, A et al. Utilizing fluorescent life time imaging microscopy technology for identify carriers of BRCA2 mutation. Biochem Biophys Res Commun 2016;480:36–41Google Scholar
24Gershanov, S, Michowiz, S, Toledano, H, Yahav, G, Barinfeld, O, Hirshberg, A et al. Fluorescence lifetime imaging microscopy, a novel diagnostic tool for metastatic cell detection in the cerebrospinal fluid of children with medulloblastoma. Sci Rep 2017;7:3648Google Scholar
25Giubileo, F, Colao, A, Puiu, G, Panzironi, G, Brizzi, F, Rocchini, P. Fluorescence spectroscopy of normal and follicular cancer samples from human thyroid. Spectroscopy 2005;19:79–87Google Scholar
26Brandao, M, Iwakura, R, Basilio, F, Haleplian, K, Ito, A, de Freitas, LC et al. Fluorescence lifetime of normal, benign, and malignant thyroid tissues. J Biomed Opt 2015;20:1–6Google Scholar
27Magde, D, Rojas, GE, Seybold, PG. Solvent dependence of the fluorescence lifetimes of xanthene dyes. Photochem Photobiol 1999;70:737–44Google Scholar