Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-10T10:14:40.011Z Has data issue: false hasContentIssue false
  • English
  • Français

Use of protein electrophoresis in the diagnosis of cerebrospinal fluid rhinorrhoea

Published online by Cambridge University Press:  29 June 2007

M. J. Porter ,
G. B. Brookes ,
A. Z. J. Zeman  and
G. Keir
M. J. Porter*
Affiliation:
Departments of Neuro-otology and Clinical Neurochemistry, The National Hospital for Neurology and Neurosurgery, Queen Square. London WCIN 3BG.
G. B. Brookes
Affiliation:
Departments of Neuro-otology and Clinical Neurochemistry, The National Hospital for Neurology and Neurosurgery, Queen Square. London WCIN 3BG.
A. Z. J. Zeman
Affiliation:
Departments of Neuro-otology and Clinical Neurochemistry, The National Hospital for Neurology and Neurosurgery, Queen Square. London WCIN 3BG.
G. Keir
Affiliation:
Departments of Neuro-otology and Clinical Neurochemistry, The National Hospital for Neurology and Neurosurgery, Queen Square. London WCIN 3BG.
*
Mr M. J. Porter, F.R.C.S., Royal National Throat Nose and Ear Hospital, Grays Inn Road, London WC1X8DA.
Get access Rights & Permissions [Opens in a new window]

Abstract

The diagnosis of CSF rhinorrhoea on clinical grounds alone can be difficult. We describe how the use of noninvasive electrophoretic analysis of nasal secretions for tau protein (asialotransferrin) helped in the management of cases where the existence of a CSF leak was in doubt. Patients were thus saved unnecessary invasive investigations or surgery. A modification of the method of analysis, which improves diagnostic accuracy, is described.

Keywords

Cerebrospinal fluidElectrophoresis
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 106 , Issue 6 , June 1992 , pp. 504 - 506
Copyright
Copyright © JLO (1984) Limited 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bentivoglio, P., Cheeseman, A. D., Symon, L. (1988) Surgical management of acoustic neuromas during the past five years. Part 1. Surgical Neurology, 29: 197–204.CrossRefGoogle Scholar
Bleach, N. R., Stanworth, P. A., Stansbie, J. M. (1988) Spontaneous cerebrospinal fluid rhinorrhoea (An investigative problem). Journal of Laryngology and Otology, 102: 633–635.CrossRefGoogle ScholarPubMed
Brockbank, M. J., Veitch, D. Y., Thompson, H. G. (1989) Cerebrospinal fluid in the rhinitis clinics. Journal of Laryngology and Otology, 103: 281–283.CrossRefGoogle Scholar
Calcaterra, T. C. (1985) Diagnosis and management of ethmoid cerebrospinal rhinorrhoea. Otolaryngologic Clinics of North America, 1: 99–105.CrossRefGoogle Scholar
Felgenhauer, K., Schadlich, H. J., Nekic, N. (1987) Beta-trace protein as a marker for CSF fistula. Klinische Wochenschrift, 65: 764–768.CrossRefGoogle Scholar
Herbert, J., Wilcox, J. N., Pham, K.-T. C. (1986) Transthyretic: a choroid plexus-specific transport protein in human brain. Neurology, 36: 900–911.CrossRefGoogle ScholarPubMed
Injala, K., Suonpaa, J., Laurent, B. (1979) Identification of CSF leakage by immunofixation. Archives of Otolaryngology. 105: 447–448.Google Scholar
Jeppsson, J. O., Laurell, C. -B., Franzen, B. (1979) Agarose gel electrophoresis. Clinical Chemistry, 25: 629–638.CrossRefGoogle ScholarPubMed
Keir, G., Zeman, A. Z. J., Brookes, G. B., Porter, M. J., Thompson, E. (1992) Immunoblotting of transferrin in the identification of cerebrospinal fluid otorrhoea and rhinorrhoea. Annals of Clinical Biochemistry, 29: 210–213.CrossRefGoogle ScholarPubMed
Liu-Shindo, M., Hawkins, D. B. (1989) Basilar skull fractures in children. International Journal of Paediatric Otorhinolaryngology, 17: 109–117.CrossRefGoogle ScholarPubMed
Lofberg, H., Grubb, A. O. (1979) Quantitation of gamma-trace in human biological fluids: Indications for production in the central nervous system. Scandanavian Journal of Clinical Laboratory Investigation, 39: 619–626.CrossRefGoogle ScholarPubMed
Oberascher, G. (1988a) Cerebrospinal fluid otorrhoea–new trends in diagnosis. American Journal of Otology, 9: 102–108.Google ScholarPubMed
Oberascher, G. (1988b) A modern concept of cerebrospinal fluid diagnosis in oto- and rhinorrhoea. Rhinology, 26: 89–103.Google Scholar
O'Connor, A. F, Luxon, L. M., Shortman, R. C. Thompson, E. J., Morrison, A. W. (1982) Electrophoretic separation and identification of perilymph proteins in cases of acoustic neuroma. Acta Otolaryngologica (Stockholm): 93: 195–200.CrossRefGoogle ScholarPubMed
Shiobara, R., Ohira, T., Kanzaki, J., Toya, S. (1988) A modified expanded middle cranial fossa approach for acoustic nerve tumours. Results of 125 operations. Journal of Neurosurqery. 68: 358–365.CrossRefGoogle Scholar
Tolley, N. S., Brookes, G. B. The surgical management of CSF rhinorrhoea. Journal of the Royal College of Surgeons of Edinburgh (in press).Google Scholar
Tripathi, R. C., Millande, C. B., Tripathi, B. J., Noronha, A. (1990) Tau fraction of transferrin is present in human aqueous humor and is not unique to cerebrospinal fluid. Experimental Eye Research, 49: 541–547.CrossRefGoogle Scholar
Verheecke, P. (1975) On the tau-protein of cerebrospinal fluid. Journal of Neurological Science, 26: 277–281.CrossRefGoogle ScholarPubMed
Walton, J. (1985) Head injuries. Chapter 5 in Brain's Diseases of the Nervous System. Oxford University Press: Oxford p. 231.Google Scholar