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Alginate-honey bioinks with improved cell responses for applications as bioprinted tissue engineered constructs

Published online by Cambridge University Press:  28 June 2018

Sudipto Datta ,
Ripon Sarkar ,
Veena Vyas ,
Sumant Bhutoria ,
Ananya Barui ,
Amit Roy Chowdhury  and
Pallab Datta
Sudipto Datta
Affiliation:
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology Shibpur, Howrah-711103, W.B., India
Ripon Sarkar
Affiliation:
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology Shibpur, Howrah-711103, W.B., India
Veena Vyas
Affiliation:
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology Shibpur, Howrah-711103, W.B., India
Sumant Bhutoria
Affiliation:
Alfatek Systems, Kolkata-700033, W.B., India
Ananya Barui
Affiliation:
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology Shibpur, Howrah-711103, W.B., India
Amit Roy Chowdhury
Affiliation:
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology Shibpur, Howrah-711103, W.B., India; and Department of Aerospace Engineering and Applied Mechanics, Indian Institute of Engineering Science and Technology Shibpur, Howrah-711103, W.B., India
Pallab Datta*
Affiliation:
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology Shibpur, Howrah-711103, W.B., India
*
a)Address all correspondence to this author. e-mail: pd@chest.iiests.ac.in, contactpallab@gmail.com
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Abstract

The polysaccharide alginate has received most extensive attention as bioink in bioprinting applications due to its ability to undergo gelation under cell-friendly conditions. However, absence of cell-binding motifs and the erratic degradation of alginate hydrogels have remained their persistent limitations. Honey is a conveniently available natural material, known for its role in wound healing and skin tissue regeneration. However, honey blending to improve biological response of alginate-based bioprinted scaffolds has not been yet reported. In the present work, honey-alginate bioinks were evaluated for their printability property (shape fidelity). It was found that honey blending reduced alginate viscosity, which gradually affected bioprinting fidelity. Therefore, the concentration that provides for acceptable bioprinting along with improvement in cell proliferations is determined. It is concluded that honey blending improves cell response of alginate bioinks and can be a facile approach to obtain bioinks especially for in situ skin tissue engineering applications.

Keywords

biologicalbiomaterialbiomedical
Type
Article
Information
Journal of Materials Research , Volume 33 , Issue 14: Focus Issue: 3D Printing of Biomaterials , 27 July 2018 , pp. 2029 - 2039
Copyright
Copyright © Materials Research Society 2018 

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