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Assessment of cell proliferation on 6H–SiC biofunctionalized with self-assembled monolayers

Published online by Cambridge University Press:  31 July 2012

A. Oliveros ,
C.L. Frewin ,
S.J. Schoell ,
M. Hoeb ,
M. Stutzmann ,
I.D. Sharp  and
S.E. Saddow
A. Oliveros*
Affiliation:
Electrical Engineering Department, University of South Florida, Tampa, Florida 33620
C.L. Frewin
Affiliation:
Department of Molecular Pharmacology and Physiology, University of South Florida, Tampa, Florida 33612
S.J. Schoell
Affiliation:
Walter Schottky Institut and Physik Department, Technische Universität München, Garching 85748, Germany
M. Hoeb
Affiliation:
Walter Schottky Institut and Physik Department, Technische Universität München, Garching 85748, Germany
M. Stutzmann
Affiliation:
Walter Schottky Institut and Physik Department, Technische Universität München, Garching 85748, Germany
I.D. Sharp
Affiliation:
Walter Schottky Institut and Physik Department, Technische Universität München, Garching 85748, Germany
S.E. Saddow
Affiliation:
Electrical Engineering Department, University of South Florida, Tampa, Florida 33620; and Department of Molecular Pharmacology and Physiology, University of South Florida, Tampa, Florida 33612
*
a)Address all correspondence to this author. e-mail: amolive4@mail.usf.edu
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Abstract

In this article, the biofunctionalization of 6H–SiC (0001) surfaces via self-assembled monolayers (SAMs) has been studied as a means to modify the in vitro biocompatibility of this semiconductor substrate with H4 (human neuroglioma) and PC12 (rat pheochromocytoma) cells. Silanization with aminopropyldiethoxymethylsilane (APDEMS) and aminopropyltriethoxysilane (APTES), which provided moderately hydrophilic surfaces, and alkylation with 1-octadecene that produced hydrophobic surfaces were used to control the 6H–SiC surface chemistry and evaluate changes in cell viability and morphology due to these surface modifications. The morphology of the cells was evaluated with atomic force microscopy. In addition, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were used to quantitatively evaluate the cell viability on the SAM-modified surfaces. In all cases, the cell proliferation was observed to improve with respect to untreated 6H–SiC surfaces, with up to a 2x increase in viability on the 1-octadecene-modified surfaces, up to 6x increase with APDEMS-modified surfaces, and up to 8x increase with APTES-modified surfaces. This proves the potential of SiC as a substrate for medical devices given the possibility to tailor its surface chemistry for specific applications.

Keywords

biomedical materialsself-assembled monolayerssilicon carbide
Type
Articles
Information
Journal of Materials Research , Volume 28 , Issue 1: Focus Issue: Silicon Carbide – Materials, Processing and Devices , 14 January 2013 , pp. 78 - 86
Copyright
Copyright © Materials Research Society 2012

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