Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-28T06:19:11.220Z Has data issue: false hasContentIssue false
  • English
  • Français

Biocomposites based on collagen and phosphorylated dextran for bone regeneration

Published online by Cambridge University Press:  14 March 2012

M.G. Albu ,
V. Trandafir ,
D.M. Suflet ,
G.C. Chitanu ,
P. Budrugeac  and
I. Titorencu
M.G. Albu*
Affiliation:
Collagen Department, INCDTP—Division Leather and Footwear Research Institute, Bucharest 031215, Romania
V. Trandafir
Affiliation:
Collagen Department, INCDTP—Division Leather and Footwear Research Institute, Bucharest 031215, Romania
D.M. Suflet
Affiliation:
Laboratory of Bioactive and Biocompatible Polymers, “Petru Poni” Institute of Macromolecular Chemistry, Iasi 700487, Romania
G.C. Chitanu
Affiliation:
Laboratory of Bioactive and Biocompatible Polymers, “Petru Poni” Institute of Macromolecular Chemistry, Iasi 700487, Romania
P. Budrugeac
Affiliation:
Laboratory of Determination of Thermal Behaviour of the Solid Products and Materials by Thermal Analysis Methods, National Institute for Research and Development for Electrical Engineering (INCDIE ICPE-CA), Bucharest 030138, Romania
I. Titorencu
Affiliation:
Department of Stem Cell Research and In Vitro Models, Institute of Cellular Biology and Pathology, Bucharest 050568, Romania
*
a)Address all correspondence to this author. e-mail: albu_mada@yahoo.com
Get access

Abstract

The aim of this study was the development of biocomposite scaffolds (membranes and matrices) based on natural polymers used for bone tissue engineering. The novelty featured in this paper is the use of phosphorylated dextran (PDex) as natural component in collagen-based biocomposites. The PDex both in acid form and as mixed salts of Mg–Na, Zn–Na, Ca-Na was characterized by Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR) spectroscopy, potentiometric and conductometric titration and energy dispersive x-ray spectroscopy (EDX) analysis. The biocomposite scaffolds were obtained by freeze-drying as matrices and by free-drying as membranes with specific microporous morphological structures that depended on drying process of collagen gels with PDex. The biocomposites were physical–chemical characterized by differential scanning calorimetry (DSC) and, water and water vapor absorption. The biocompatibility was evaluated in vitro with human osteosarcoma MG 63 cell lines. The results showed that biocompatibility was improved by the use of PDex as mixed salts of Mg–Na, Zn–Na, Ca-Na in collagen biocomposites.

Type
Articles
Information
Journal of Materials Research , Volume 27 , Issue 7 , 14 April 2012 , pp. 1086 - 1096
Copyright
Copyright © Materials Research Society 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1.Iordachescu, D., Campean, A., and Stan, C.: Extracelular Matrix Modeling. Applications in Tissue Engineering (Pro-Transilvania Press, Bucharest, Romania, 2004) p. 15.Google Scholar
2.Yoshida, T., Yasuda, Y., Uryu, T., Nakashima, H., Yamamoto, N., Mimura, T., and Kaneko, Y.: Synthesis and in vitro inhibitory effect of L-glycosyl-branched curdlan sulfates on AIDS virus infection. Macromolecules 27, 6272 (1994).CrossRefGoogle Scholar
3.Gao, Y., Fukuda, A., Katsuraya, K., Kaneko, Y., Mimura, T., Nakashima, H., and Uryu, T.: Synthesis of regioselective substituted curdlan sulfates with medium molecular weights and their specific anti-HIV-1 activities. Macromolecules 30, 3224 (1997).CrossRefGoogle Scholar
4.Gao, Y., Katsuraya, K., Kaneko, Y., Mimura, T., Nakashima, H., and Uryu, T.: Synthesis, enzymatic hydrolysis, and anti-HIV activity of AZT−spacer−curdlan sulfates. Macromolecules 32, 8319 (1999).CrossRefGoogle Scholar
5.Koumoto, K., Sakurai, K., Shinkai, S., and Kunitake, T.: Chemical modification of curdlan: Characteristic complexation with polynucleotides by control of the molecular weight. Polym. Preprints 43, 721 (2002).Google Scholar
6.Jeon, K.J., Katsuraya, K., Inazu, T., Kaneko, Y., Mimura, T., and Uryu, T.: NMR spectroscopic detection of interactions between a HIV protein sequence and a highly anti-HIV active curdlan sulfate. J. Am. Chem. Soc. 122, 125 (2000).CrossRefGoogle Scholar
7.Shimabayashi, S., Hashimoto, N., Kawamura, H., and Uno, T.: Formation of hydroxyapatite in the presence of phosphorylated and sulfated polymer in an aqueous phase. Mineral Scale Formation and Inhibition, edited by Zahid, A. (Plenum Press, New York, NY, 1995) p. 77.Google Scholar
8.Ficai, A., Andronescu, E., Trandafir, V., Ghitulica, C., and Voicu, G.: Collagen/hydroxyapatite composite obtained by electric field orientation. Mater. Lett. 64, 541 (2010).CrossRefGoogle Scholar
9.Ficai, A., Andronescu, E., Voicu, G., Ghitulica, C., and Ficai, D.: The influence of collagen support and ionic species on the morphology of collagen/hydroxyapatite composite materials. Mater. Charact. 61, 402 (2010).CrossRefGoogle Scholar
10.Ficai, A., Andronescu, E., Voicu, G., Ghitulica, C., Vasile, B.S., Ficai, D., and Trandafir, V.: Self assembled collagen/hydroxyapatite composite materials. Chem. Eng. J. 160, 794 (2010).CrossRefGoogle Scholar
11.Ficai, M., Andronescu, E., Ficai, D., Voicu, G., and Ficai, A.: Synthesis and characterization of COLL-PVA/HA hybrid materials with stratified morphology. Colloids Surf., B 81, 614 (2010).CrossRefGoogle ScholarPubMed
12.Granja, P.L., Barbosa, M.A., Pouysegu, L., De Jeso, B., and Baquey, C.: Cellulose Phosphates as Biomaterials. Frontiers in biomedical polymer applications, edited by Ottenbrite, R.M. (Technomic Press, Lancaster, PA, 1999) p. 195.Google Scholar
13.Volkin, D.B., Tsai, P.K., Dabora, J.M., Gress, J.O., Burke, C.J., Linhardt, R.J., and Middaugh, C.R.: Physical stabilization of acidic fibroblast growth factor by polyanions. Arch. Biochem. Biophys. 300(1), 30 (1993).CrossRefGoogle ScholarPubMed
14.Heinze, T., Liebert, T., Heublein, B., and Hornig, S.: Functional polymers base on dextran. Adv. Polym. Sci. 205, 199 (2006).CrossRefGoogle Scholar
15.Robyt, J.F.: Dextran. Encyclopedia of polymer science and technology, edited by Kroschwitz, I. (John Wiley & Sons, New York, NY, 1987) p. 752.Google Scholar
16.Jing, W., Wu, L., Lin, Y., Liu, L., Tang, W., and Tian, W.: Odontogenic differentiation of adipose-derived stem cells for tooth regeneration: Necessity, possibility, and strategy. Med. Hypotheses 70, 540 (2008).CrossRefGoogle ScholarPubMed
17.Duailibi, M.T., Duailibi, S.E., Young, C.S., Bartlett, J.D., Vacanti, J.P., and Yelick, P.C.: Bioengineered teeth from cultured rat tooth bud cells. J. Dent. Res. 83, 523 (2004).CrossRefGoogle ScholarPubMed
18.Sacco, D., Klett-Zygmunt, D., and Dellacherie, E.: A re-investigation of the phosphorylation of dextran with phosphoric acid: Evidence for the formation of different types of phosphate moieties. Carbohydr. Res. 184, 193 (1988).CrossRefGoogle Scholar
19.Sacco, D., Klett-Zygmunt, D., Vigneron, C., and Dellacherie, E.: Covalent fixation of hemoglobin to dextran phosphates decreases its oxygen affinity. Biochim. Biophys. Acta 1041, 279 (1990).CrossRefGoogle ScholarPubMed
20.Spaltro, S.M. and Aronson, M.P.: Phosphorylated polyhydroxy compounds for tartar control. Patent No 5 12 599, (1992).Google Scholar
21.Suzuki, S., Suzuki, M., and Matsumoto, T.: Polysaccharides bonded with phosphoric acid and fatty acid esters. Patent No 50 054 685, (1975).Google Scholar
22.Suzuki, S., Suzuki, M., and Mikami, T.: Esters of polysaccharides with phosphoric acid and palmitic acid. Patent No JP 75–104,626, (1977).Google Scholar
23.Makarova, S.B., Aptova, T.A., Litvak, Z.M., and Raldugina, T.F.: Ion exchanger. Patent No 59 768, (1978).Google Scholar
24.Sato, T., Nishimura-Uemura, J., Shimosato, T., Kawai, Y., Kitazawa, H., and Saito, T.: Dextran from Leuconostoc mesenteroides augments immunostimulatory effects by the introduction of phosphate groups. J. Food Prot. 67, 1719 (2004).CrossRefGoogle ScholarPubMed
25.Suflet, D.M., Chitanu, G.C., and Desbrières, J.: Phosphorylated polysaccharides. 2. Synthesis and properties of phosphorylated dextran. Carbohydr. Polym. 82, 1271 (2010).CrossRefGoogle Scholar
26.Suflet, D.M., Chitanu, G.C., and Popa, V.I.: Phosphorylation of polysaccharides. New results on synthesis and characterization of phosphorylated cellulose. React. Funct. Polym. 66, 1240 (2006).CrossRefGoogle Scholar
27.Iliescu, R.I., Andronescu, E., Voicu, G., Ficai, A., and Covaliu, C.I.: Hybrid materials based on montmorillonite and cytostatic drugs: Preparation and characterization. Appl. Clay Sci. 52(1–2), 62–68 (2011).CrossRefGoogle Scholar
28.Trandafir, V., Popescu, G., Albu, M.G., Iovu, H., and Georgescu, M.: Bioproducts Based on Collagen (Ars Docendi Press, Bucharest, Romania, 2007) p. 102.Google Scholar
29.Siapi, E., Mavromoustakos, T., Trandafir, V., Albu, B., and Budrugeac, P.: The use of differential scanning calorimetry to study the effects of gentamycin on fibrous collagenous membranes. Thermochim. Acta 425, 165 (2005).CrossRefGoogle Scholar
30.Albu, M.G.: Collagen Gels and Matrices for Biomedical Applications (Lambert Academic Publishing, Saarbrücken, Germany, 2011) p. 23.Google Scholar
31.Purama, R.K., Goswami, P., Khan, A.T., and Goyal, A.: Structural analysis and properties of dextran produced by Leuconostoc mesenteroides NRRL B-640. Carbohydr. Polym. 76, 30 (2009).CrossRefGoogle Scholar
32.Hesse, M., Meier, H., and Zeeh, B.: Spectroscopic Methods in Organic Chemistry (Georg Thieme Verlag Stuttgart Press, New York, NY, 1984) p. 40.Google Scholar
33.Budrugeac, P., Trandafir, V., and Albu, M.G.: The effect of the hydration degree on the hydrotermal and thermo-oxidative stability of some collagenous matrices. J. Therm. Anal. Calorim. 72, 581 (2003).CrossRefGoogle Scholar
34.Okamoto, Y. and Saeki, K.: Phase transition on collagen and gelatin. Kolloid Z. Z. Polym. 194, 124 (1964).CrossRefGoogle Scholar
35.Badea, E., Miu, L., Budrugeac, P., Giurginca, M., Mašić, A., Badea, N., and Della Gatta, G.: Study of deterioration of historical parchments by various thermal analysis techniques, complemented by SEM, FTIR, UV-VIS-NIR and unilateral NMR investigations. J. Therm. Anal. Calorim. 91, 17 (2008).CrossRefGoogle Scholar
36.Budrugeac, P. and Miu, L.: The suitability of DSC method for damage assessment and certification of historical leathers and parchments. J. Cult. Herit. 9, 146 (2008).CrossRefGoogle Scholar
37.Popescu, C., Budrugeac, P., Wortmann, F.J., Miu, L., Demco, D., and Baias, M.: Assessment of collagen-based materials which are supports of cultural and historical objects. Polym. Degrad. Stab. 93, 976 (2008).CrossRefGoogle Scholar
38.Loke, W.K. and Khor, E.: Validation of the shrinkage temperature of animal tissue for bioprosthetic heart valve application by differential scanning calorimetry. Biomater. 16, 251 (1995).CrossRefGoogle ScholarPubMed
39.Albu, M.G., Ghica, M.V., Ficai, A., Titorencu, I., and Popa, L.: The influence of freeze-drying process on porosity and kinetics release of collagen-doxycycline matrices, edited by Albu, L. and Deselnicu, V. (Proc. 3rd Int. Conf. Adv. Mat. Syst, Bucharest, Romania, 2010) p. 181.Google Scholar
40.Chirita, Gh., and Chirita, M.: Treatise of Leather and Fur Chemistry and Technology (Gheorghe Asachi Publishing House, Iaşi, Romania, 1999) pp. 142–144.Google Scholar
41.Roach, P., Eglin, D., Rohde, K., and Perry, C.C.: Modern biomaterials: A review—bulk properties and implications of surface modifications. J. Mater. Sci. Mater. Med. 18, 1263 (2006).CrossRefGoogle Scholar