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Bioprosthetic heart valves’ structural integrity improvement through exogenous amino donor treatments

Published online by Cambridge University Press:  05 July 2018

Yang Lei Open the ORCID record for Yang Lei [Opens in a new window] ,
Wanyu Jin ,
Rifang Luo ,
Gaocan Li ,
Gaoyang Guo  and
Yunbing Wang
Yang Lei*
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China
Wanyu Jin
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China
Rifang Luo
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China
Gaocan Li
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China
Gaoyang Guo
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China
Yunbing Wang*
Affiliation:
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China
*
a)Address all correspondence to these authors. e-mail: leiyang@scu.edu.cn
b)e-mail: yunbing.wang@scu.edu.cn
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Abstract

Valvular heart diseases lead to over 300,000 heart valve replacements worldwide each year. Bioprosthetic heart valves (BHVs), derived from glutaraldehyde (GLUT) crosslinked porcine or bovine pericardium, are often used. However, valve failure can occur within 12–15 years due to progressive degradation and/or calcification. Being innovated by previous amino reagent studies used for GLUT detoxification and carbodiimide [1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, EDC] chemistry, in this study, we developed a new fabrication method that utilizes exogenous amino donor arginine or lysine carbodiimide combined treatments to better stabilize the extracellular matrix of porcine pericardium. The carboxyl group density, amine content, differential scanning calorimetry, collagenase and elastase degradation, calcification by rat subdermal implantation, cytotoxicity, and platelet adhesion were characterized. We demonstrated that exogenous amino donor carbodiimide combined treatment for pericardiums had better resistance to elastase degradation (1.63 ± 0.11% and 1.44 ± 0.24% in arginine or lysine versus 3.68 ± 0.16% and 3.04 ± 0.11% in GLUT and GLUT/EDC control) and calcification (0.624 ± 0.193 and 0.637 ± 0.213 Ca µg/mg tissue in arginine or lysine versus 1.610 ± 0.124 and 1.512 ± 0.075 Ca µg/mg tissue in GLUT and GLUT/EDC control). This new strategy combined arginine or lysine and carbodiimide crosslinking would be a promising method to produce more robust BHVs with better structural stability and anticalcification property.

Keywords

bioprosthetic heart valvespericardiumstructural integrityextracellular matrixcalcificationamino donor
Type
Article
Information
Journal of Materials Research , Volume 33 , Issue 17 , 14 September 2018 , pp. 2576 - 2585
Copyright
Copyright © Materials Research Society 2018 

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