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Electrohydrodynamic-jetting (EHD-jet) 3D-printed functionally graded scaffolds for tissue engineering applications

Published online by Cambridge University Press:  04 June 2018

Sanjairaj Vijayavenkataraman Open the ORCID record for Sanjairaj Vijayavenkataraman [Opens in a new window] ,
Shuo Zhang ,
Wen Feng Lu  and
Jerry Ying Hsi Fuh
Sanjairaj Vijayavenkataraman*
Affiliation:
Department of Mechanical Engineering, National University of Singapore, Singapore 117576, Singapore
Shuo Zhang
Affiliation:
Department of Mechanical Engineering, National University of Singapore, Singapore 117576, Singapore
Wen Feng Lu
Affiliation:
Department of Mechanical Engineering, National University of Singapore, Singapore 117576, Singapore
Jerry Ying Hsi Fuh
Affiliation:
Department of Mechanical Engineering, National University of Singapore, Singapore 117576, Singapore
*
a)Address all correspondence to this author. e-mail: vijayavenkataraman@u.nus.edu
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Abstract

Biomimicry is a desirable quality of tissue engineering scaffolds. While most of the scaffolds reported in the literature contain a single pore size or porosity, the native biological tissues such as cartilage and skin have a layered architecture with zone-specific pore size and mechanical properties. Thus, there is a need for functionally graded scaffolds (FGS). EHD-jet 3D printing is a high-resolution process and a variety of polymer solutions can be processed into 3D porous scaffolds at ease, overcoming the limitations of other 3D printing methods (SLS, stereolithography, and FDM) in terms of resolution and limited material choice. In this paper, a novel proof of concept study on fabrication of porous polycaprolactone-based FGS by using EHD-jet 3D printing technology is presented. Organomorphic scaffolds, multiculture systems, interfacial tissue engineering, and in vitro cancer metastasis models are some of the futuristic applications of these polymeric FGS.

Keywords

E-jettingfunctionally graded scaffoldsgradient porosityvariable porositytissue engineeringtissue engineering scaffoldsPCL scaffolds3D printing
Type
Invited Article
Information
Journal of Materials Research , Volume 33 , Issue 14: Focus Issue: 3D Printing of Biomaterials , 27 July 2018 , pp. 1999 - 2011
Copyright
Copyright © Materials Research Society 2018 

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References

REFERENCES

Vijayavenkataraman, S., Lu, W., and Fuh, J.: 3D bioprinting of skin: A state-of-the-art review on modelling, materials, and processes. Biofabrication 8, 032001 (2016).CrossRefGoogle ScholarPubMed
Vijayavenkataraman, S., Lu, W., and Fuh, J.: 3D bioprinting—An ethical, legal and social aspects (ELSA) framework. Bioprinting 1, 11 (2016).CrossRefGoogle Scholar
Hollister, S.J.: Porous scaffold design for tissue engineering. Nat. Mater. 4, 518 (2005).CrossRefGoogle ScholarPubMed
O’brien, F.J.: Biomaterials & scaffolds for tissue engineering. Mater. Today 14, 88 (2011).CrossRefGoogle Scholar
Vijayavenkataraman, S., Shuo, Z., Fuh, J.Y., and Lu, W.F.: Design of three-dimensional scaffolds with tunable matrix stiffness for directing stem cell lineage specification: An in silico study. Bioengineering 4, 66 (2017).CrossRefGoogle Scholar
Leong, K., Chua, C., Sudarmadji, N., and Yeong, W.: Engineering functionally graded tissue engineering scaffolds. J. Mech. Behav. Biomed. Mater. 1, 140 (2008).CrossRefGoogle ScholarPubMed
Woodfield, T., Blitterswijk, C.V., Wijn, J.D., Sims, T., Hollander, A., and Riesle, J.: Polymer scaffolds fabricated with pore-size gradients as a model for studying the zonal organization within tissue-engineered cartilage constructs. Tissue Eng. 11, 1297 (2005).CrossRefGoogle ScholarPubMed
Sharma, B., Williams, C.G., Kim, T.K., Sun, D., Malik, A., Khan, M., Leong, K., and Elisseeff, J.H.: Designing zonal organization into tissue-engineered cartilage. Tissue Eng. 13, 405 (2007).CrossRefGoogle ScholarPubMed
Bracaglia, L.G., Smith, B.T., Watson, E., Arumugasaamy, N., Mikos, A.G., and Fisher, J.P.: 3D printing for the design and fabrication of polymer-based gradient scaffolds. Acta Biomater. 56, 3–13 (2017).CrossRefGoogle ScholarPubMed
Miao, X. and Sun, D.: Graded/gradient porous biomaterials. Materials 3, 26 (2009).CrossRefGoogle Scholar
Ma, P.X.: Scaffolds for tissue fabrication. Mater. Today 7, 30 (2004).CrossRefGoogle Scholar
Lu, T., Li, Y., and Chen, T.: Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering. Int. J. Nanomed. 8, 337 (2013).CrossRefGoogle ScholarPubMed
Thieme, M., Wieters, K-P., Bergner, F., Scharnweber, D., Worch, H., Ndop, J., Kim, T., and Grill, W.: Titanium powder sintering for preparation of a porous functionally graded material destined for orthopaedic implants. J. Mater. Sci.: Mater. Med. 12, 225 (2001).Google ScholarPubMed
Miao, X., Hu, Y., Liu, J., Tio, B., Cheang, P., and Khor, K.A.: Highly interconnected and functionally graded porous bioceramics. In Key Engineering Materials, Vol. 240, edited by Ben-Nissan, B., Sher, D., and Walsh, W.. (Trans Tech Publications, Zurich, Switzerland, 2003); p. 595.Google Scholar
Macchetta, A., Turner, I.G., and Bowen, C.R.: Fabrication of HA/TCP scaffolds with a graded and porous structure using a camphene-based freeze-casting method. Acta Biomater. 5, 1319 (2009).CrossRefGoogle ScholarPubMed
Suk, M-J., Choi, S-I., Kim, J-S., Do Kim, Y., and Kwon, Y-S.: Fabrication of a porous material with a porosity gradient by a pulsed electric current sintering process. Met. Mater. Int. 9, 599 (2003).CrossRefGoogle Scholar
An, J., Teoh, J.E.M., Suntornnond, R., and Chua, C.K.: Design and 3D printing of scaffolds and tissues. Engineering 1, 261 (2015).CrossRefGoogle Scholar
Do, A.V., Khorsand, B., Geary, S.M., and Salem, A.K.: 3D printing of scaffolds for tissue regeneration applications. Adv. Healthcare Mater. 4, 1742 (2015).CrossRefGoogle ScholarPubMed
Liu, F-H.: Synthesis of biomedical composite scaffolds by laser sintering: Mechanical properties and in vitro bioactivity evaluation. Appl. Surf. Sci. 297, 1 (2014).CrossRefGoogle Scholar
Sabnis, A., Rahimi, M., Chapman, C., and Nguyen, K.T.: Cytocompatibility studies of an in situ photopolymerized thermoresponsive hydrogel nanoparticle system using human aortic smooth muscle cells. J. Biomed. Mater. Res., Part A 91, 52 (2009).CrossRefGoogle Scholar
Vijayavenkataraman, S.: A perspective on bioprinting ethics. Artif. Organs 40, 1033 (2016).CrossRefGoogle ScholarPubMed
Hutmacher, D.W., Schantz, T., Zein, I., Ng, K.W., Teoh, S.H., and Tan, K.C.: Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling. J. Biomed. Mater. Res., Part A 55, 203 (2001).3.0.CO;2-7>CrossRefGoogle ScholarPubMed
Park, A., Wu, B., and Griffith, L.G.: Integration of surface modification and 3D fabrication techniques to prepare patterned poly(L-lactide) substrates allowing regionally selective cell adhesion. J. Biomater. Sci., Polym. Ed. 9, 89 (1998).CrossRefGoogle ScholarPubMed
Serra, T., Ortiz-Hernandez, M., Engel, E., Planell, J.A., and Navarro, M.: Relevance of PEG in PLA-based blends for tissue engineering 3D-printed scaffolds. Mater. Sci. Eng., C 38, 55 (2014).CrossRefGoogle ScholarPubMed
Khojasteh, A., Behnia, H., Hosseini, F.S., Dehghan, M.M., Abbasnia, P., and Abbas, F.M.: The effect of PCL–TCP scaffold loaded with mesenchymal stem cells on vertical bone augmentation in dog mandible: A preliminary report. J. Biomed. Mater. Res., Part B 101, 848 (2013).CrossRefGoogle ScholarPubMed
Xu, N., Ye, X., Wei, D., Zhong, J., Chen, Y., Xu, G., and He, D.: 3D artificial bones for bone repair prepared by computed tomography-guided fused deposition modeling for bone repair. ACS Appl. Mater. Interfaces 6, 14952 (2014).CrossRefGoogle ScholarPubMed
Liu, H., Vijayavenkataraman, S., Wang, D., Jing, L., Sun, J., and He, K.: Influence of electrohydrodynamic jetting parameters on the morphology of PCL scaffolds. Int. J. Bioprint. 3, 72 (2017).CrossRefGoogle Scholar
Sun, J., Vijayavenkataraman, S., and Liu, H.: An overview of scaffold design and fabrication technology for engineered knee meniscus. Materials 10, 29 (2017).CrossRefGoogle ScholarPubMed
Wang, H., Vijayavenkataraman, S., Wu, Y., Shu, Z., Sun, J., and Hsi, J.F.Y.: Investigation of process parameters of electrohydro-dynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries. Int. J. Bioprint. 2, 63–71 (2016).CrossRefGoogle Scholar
Uth, N., Mueller, J., Smucker, B., and Yousefi, A-M.: Validation of scaffold design optimization in bone tissue engineering: Finite element modeling versus designed experiments. Biofabrication 9, 015023 (2017).CrossRefGoogle ScholarPubMed