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Influence of geometry on cell proliferation of PLA and alumina scaffolds constructed by additive manufacturing

Published online by Cambridge University Press:  11 November 2019

Jhon Alexander Ramírez Open the ORCID record for Jhon Alexander Ramírez [Opens in a new window] ,
Valentina Ospina Open the ORCID record for Valentina Ospina [Opens in a new window] ,
Angie A. Rozo Open the ORCID record for Angie A. Rozo [Opens in a new window] ,
Maria I. Viana ,
Sebastian Ocampo ,
Sebastian Restrepo ,
Neil A. Vásquez ,
Carlos Paucar  and
Claudia García Open the ORCID record for Claudia García [Opens in a new window]
Jhon Alexander Ramírez
Affiliation:
Grupo de Cerámicos y vítreos, Universidad Nacional de Colombia sede Medellín, Medellín 050034, Colombia
Valentina Ospina
Affiliation:
Laboratorio de Genética, Grupo de Biotecnología Animal, Universidad Nacional de Colombia sede Medellín, Medellín 050034, Colombia
Angie A. Rozo
Affiliation:
Laboratorio de Genética, Grupo de Biotecnología Animal, Universidad Nacional de Colombia sede Medellín, Medellín 050034, Colombia
Maria I. Viana
Affiliation:
Laboratorio de Genética, Grupo de Biotecnología Animal, Universidad Nacional de Colombia sede Medellín, Medellín 050034, Colombia
Sebastian Ocampo
Affiliation:
Grupo de Cerámicos y vítreos, Universidad Nacional de Colombia sede Medellín, Medellín 050034, Colombia
Sebastian Restrepo
Affiliation:
Grupo de Cerámicos y vítreos, Universidad Nacional de Colombia sede Medellín, Medellín 050034, Colombia
Neil A. Vásquez
Affiliation:
Laboratorio de Genética, Grupo de Biotecnología Animal, Universidad Nacional de Colombia sede Medellín, Medellín 050034, Colombia
Carlos Paucar
Affiliation:
Grupo de Cerámicos y vítreos, Universidad Nacional de Colombia sede Medellín, Medellín 050034, Colombia
Claudia García*
Affiliation:
Grupo de Cerámicos y vítreos, Universidad Nacional de Colombia sede Medellín, Medellín 050034, Colombia
*
a)Address all correspondence to this author. e-mail: cpgarcia@unal.edu.co
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Abstract

Scaffolds based on two different geometries were constructed by additive manufacturing: one based on a triply periodic minimal surface, the Schwarz D surface, and the other based on a rectangular geometry with orthogonal through-holes. For construction of the scaffolds, two different materials were used: polylactic acid (PLA) in filament form and alumina in printable paste form. The structure of the resulting scaffolds was characterized via X-ray diffraction and scanning electron microscopy, and cell proliferation was assessed for each geometry and material, using fluorescence microscopy and DNA quantification via NanoDrop. Additive manufacturing allowed us to obtain scaffolds with the assessed materials while guaranteeing the interconnectivity of the pores in each one. The curved surfaces constructed with PLA were more favorable for cell attachment and proliferation of the CHO-K1 cell line.

Keywords

biomimetic (assembly)cellular (material form)tissue
Type
Invited Paper
Information
Journal of Materials Research , Volume 34 , Issue 22 , 28 November 2019 , pp. 3757 - 3765
Copyright
Copyright © Materials Research Society 2019 

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