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Nanoencapsulating living biological cells using electrostatic layer-by-layer self-assembly: Platelets as a model

Published online by Cambridge University Press:  24 January 2011

Qinghe Zhao ,
Hongshuai Li  and
Bingyun Li
Qinghe Zhao
Affiliation:
Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, West Virginia 26506
Hongshuai Li
Affiliation:
Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, West Virginia 26506
Bingyun Li*
Affiliation:
Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, West Virginia 26506; WVNano Initiative, Morgantown, West Virginia 26506; and Department of Chemical Engineering, College of Engineering and Mineral Resources, West Virginia University, Morgantown, West Virginia 26506
*
a)Address all correspondence to this author. e-mail: bli@hsc.wvu.edu
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Abstract

In the literature, a few biological cells have been used as templates to form microcapsules of a variety of shapes and sizes. In this study, we proved the concept that living cells like platelets can be encapsulated with polyelectrolytes using electrostatic layer-by-layer self-assembly (LBL), and, most importantly, the encapsulation process did not induce activation of the platelets. Glycol-chitosan and poly-L-glutamic acid were electrostatically deposited onto platelets, and the encapsulation was confirmed using confocal laser scanning microscopy and scanning electron microscopy. Transmission electron microscopy observation further confirmed that the encapsulation process was mild and the activation of platelets was negligible. The encapsulation of living biological cells like platelets can serve as a model system in a wide range of biomedical applications including local and sustained drug delivery, immune protection of artificial tissues, and versatile artificial blood.

Keywords

BiomedicalCoatingSelf-Assembly

Information

Type
Reviews
Information
Journal of Materials Research , Volume 26 , Issue 2: Focus Issue: Self-Assembly and Directed Assembly of Advanced Materials , 28 January 2011 , pp. 347 - 351
Copyright
Copyright © Materials Research Society 2011

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References

REFERENCES

1.Lanza, R.P. and Chick, W.L.: Transplantation of encapsulated cells and tissues. Surgery 121(1), 1 (1997).CrossRefGoogle ScholarPubMed
2.Decher, G.: Fuzzy nanoassemblies: Toward layered polymeric multicomposites. Science 277(5330), 1232 (1997).CrossRefGoogle Scholar
3.Li, B., Jiang, B., Boyce, B., and Lindsey, B.: Multilayer polypeptide nanoscale coatings for the prevention of biomedical device associated infections. Biomaterials 30, 2552 (2009).CrossRefGoogle ScholarPubMed
4.Li, B., Jiang, B., Dietz, M.J., Smith, E.S., Clovis, N.B., and Rao, K.M.K.: Evaluation of local MCP-1 and IL-12 nanocoatings for infection prevention in open fractures. J. Orthop. Res. 28, 48 (2010).CrossRefGoogle ScholarPubMed
5.Li, H., Ogle, H., Jiang, B., Hagar, M., and Li, B.: Cefazolin embedded biodegradable polypeptide nanofilms promising for infection prevention: A preliminary study on cell responses. J. Orthop. Res. 28, 992 (2010).CrossRefGoogle Scholar
6.Jiang, B. and Li, B.: Tunable drug incorporation and release from polypeptide multilayer nanofilms. Int. J. Nanomed. 4, 37 (2009).Google Scholar
7.Jiang, B. and Li, B.: Polypeptide nanotechnology coatings for preventing dental and orthopaedic device-associated infection. J. Biomed. Mater. Res., Part B 88(2), 332 (2009).CrossRefGoogle Scholar
8.Cortez, C., Tomaskovic-Crook, E., Johnston, A.P.R., Radt, B., Cody, S.H., Scott, A.M., Nice, E.C., Heath, J.K., and Caruso, F.: Targeting and uptake of multilayered particles to colorectal cancer cells. Adv. Mater. 18, 1998 (2006).CrossRefGoogle Scholar
9.Heuberger, R., Sukhorukov, G.B., Vörös, J., Textor, M., and Möhwald, H.: Biofunctional polyeletrolyte multilayers and microcapsules: Control of non-specific and bio-specific protein adsorption. Adv. Funct. Mater. 15, 357 (2005).CrossRefGoogle Scholar
10.Diaspro, A., Silvano, D., Krol, S., Cavalleri, O., and Gliozzi, A.: Single living cell encapsulation in nano-organized polyelectrolyte shells. Langmuir 18, 5047 (2002).CrossRefGoogle Scholar
11.Krol, S., Guerra, S., Grupillo, M., Diaspro, A., Gliozzi, A., and Marchetti, P.: Multilayer nanoencapsulation. New approach for immune protection of human pancreatic islets. Nano Lett. 6, 1933 (2006).CrossRefGoogle ScholarPubMed
12.Neu, B., Voigt, A., Mitlöhner, R., Leporatti, S., Gao, C.Y., Donath, E., Kiesewetter, H., Möhwald, H., Meiselman, H.J., and Bäumler, H.: Biological cells as templates for hollow microcapsules. J. Microencapsulation 18(3), 385 (2001).Google ScholarPubMed
13.Ai, H., Fang, M., Jones, S.A., and Lvov, Y.M.: Electrostatic layer-by-layer nanoassembly on biological microtemplates: Platelets. Biomacromolecule 3, 560 (2002).CrossRefGoogle ScholarPubMed
14.Donath, E., Moya, S., Neu, B., Sukhorukov, G.B., Georgieva, R., Voigt, A., Bäumler, H., Kiesewetter, H., and Möhwald, H.: Hollow polymer shells from biological templates: Fabrication and potential applications. Chemistry 8(23), 5481 (2002).3.0.CO;2-8>CrossRefGoogle ScholarPubMed
15.Onishi, H. and Machida, Y.: Biodegradation and distribution of water-soluble chitosan in mice. Biomaterials 20, 175 (1999).CrossRefGoogle ScholarPubMed
16.Li, H.S. and Li, B.: Platelet-rich plasma: Its biological properties and applications, in Biocompatibility of materials, devices and drug release systems, edited by Columbus, Frank (Nova Science Publishers, Inc: New York, NY, In press, 2010)Google Scholar
17.Uludag, H., De Vos, P., and Tresco, P.A.: Technology of mammalian cell encapsulation. Adv. Drug Deliv. Rev. 42, 29 (2000).CrossRefGoogle ScholarPubMed
18.Cell Encapsulation Technology and Therapeutics, edited by Kühtreiber, W.M., Lanza, R.P., and Chick, W.L. (Boston, MA: Birkhäuser, 1999).CrossRefGoogle Scholar
19.Visted, T., Bjerkvig, R., and Enger, P.O.: Cell encapsulation technology as a therapeutic strategy for CNS malignancies. Neuro-oncol. 3, 201 (2001).CrossRefGoogle ScholarPubMed
20.Zhong, Y., Li, B., and Haynie, D.T.: Fine tuning of physical properties of designed polypeptide multilayer films by control of pH. Biotechnol. Progr. 22(1), 126 (2006).CrossRefGoogle ScholarPubMed
21.Mehta, S. and Watson, J.T.: Platelet rich concentrate: Basic science and current clinical applications. J. Orthop. Trauma 22(6), 432 (2008).CrossRefGoogle ScholarPubMed
22.White, J.G.: Platelet ultrastructure, in Hemostasis and Thrombosis, 3rd ed., edited by Bloom, A.L., Forbes, C.D., Thomas, D.P., and Tuddenham, E.G.D. (Edinburgh: Churchill Livingstone, 1994).Google Scholar
23.Parise, L.V., Smyth, S.S., Shet, A.S., and Coller, B.S.: Platelet morphology, biochemistry, and function, in Williams Hematology, 7th ed., edited by Lichtman, M.A., Beutler, E., Kipps, T.J., Seligsohn, U., Kaushansky, K., and Prchal, J.T. (McGraw-Hill Companies, Inc.: Columbus, OH, 2006).Google Scholar