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Preliminary study on effect of nano-hydroxyapatite and mesoporous bioactive glass on DNA

Published online by Cambridge University Press:  12 June 2018

Itishree Ratha ,
Akrity Anand ,
Sabyasachi Chatterjee ,
Biswanath Kundu  and
Gopinatha Suresh Kumar
Itishree Ratha
Affiliation:
Bioceramics and Coating Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata 700032, India
Akrity Anand
Affiliation:
Bioceramics and Coating Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata 700032, India
Sabyasachi Chatterjee
Affiliation:
Biophysical Chemistry Laboratory, Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata 700032, India
Biswanath Kundu*
Affiliation:
Bioceramics and Coating Division, CSIR-Central Glass and Ceramic Research Institute, Kolkata 700032, India
Gopinatha Suresh Kumar
Affiliation:
Biophysical Chemistry Laboratory, Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata 700032, India
*
a)Address all correspondence to this author. e-mail: biswa_kundu@rediffmail.com
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Abstract

In this study, nano-hydroxyapatite (n-HAp) of average crystallite size ∼8.15 ± 4 nm of hexagonal geometry with size ranging between 14 and 50 nm was synthesized in laboratory at room temperature by using suitable sources of calcium and phosphate ions and using triethanolamine. Mesoporous bioactive glass (MBG) was synthesized by using cationic surfactant cetyl trimethyl ammonium bromide of the SiO2–CaO–P2O5 glass system. After calcination at 650 °C, MBG powders were having a zeta potential of −16.5 mV (pH ∼9.1), median particle size ∼75 nm, and specific surface area 473.2 m2/g. An aqueous suspension of DNA was used to disperse both n-HAp and MBG and further subjected for analysis including absorbance, circular dichroism spectroscopy, UV-melting, and isothermal titration calorimetry. Absorbance spectroscopy indicated that an equilibrium binding was obtained between both materials and DNA in solution phase. Due to the addition of the nanomaterial, molar ellipticity of DNA was changed revealing that the materials were interacted with DNA. From UV melting characterization, there is a shifting of the melting temperature of DNA in the presence of MBG and n-HAp, respectively, suggesting that the nanoparticles stabilized DNA helix to a considerable extent.

Keywords

nano-hydroxyapatitemesoporous bioactive glassDNACD spectroscopyUV-meltingisothermal titration calorimetry
Type
Article
Information
Journal of Materials Research , Volume 33 , Issue 11 , 13 June 2018 , pp. 1592 - 1601
Copyright
Copyright © Materials Research Society 2018 

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