Hostname: page-component-6bf8c574d5-956mj Total loading time: 0 Render date: 2025-02-19T05:29:15.041Z Has data issue: false hasContentIssue false
  • English
  • Français

Processing of biphasic calcium phosphate ceramics for culturing of bone marrow stem cells

Published online by Cambridge University Press:  04 April 2017

Qinghao Zhang ,
Qi Jiapeng ,
Wenfu Wang  and
Ian Nettleship
Qinghao Zhang
Affiliation:
Department of Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA 15261
Qi Jiapeng
Affiliation:
Department of Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA 15261
Wenfu Wang
Affiliation:
Department of Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA 15261
Ian Nettleship*
Affiliation:
Department of Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA 15261; and McGowan Institute of Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15261
*
a)Address all correspondence to this author. e-mail: NETTLES@pitt.edu
Get access

Abstract

Ceramic scaffolds are being developed to control both proliferation and differentiation of hematopoietic stem cells into desired cell products in bioreactors. These scaffolds mimic important aspects of the microenvironment or “niche” inside bone marrow. In particular, hematopoietic stem cell fate is thought to be effected by the architecture of trabecular bone and the presence of calcium. Here we report the effects of ceramics processing on the phase distribution and microstructure of biphasic ceramics used to culture hematopoietic stem cells. Processing of biphasic ceramics by powder mixing resulted in tetracalcium phosphate on the sintered surface. This correlated with observed surface deposits, weight gain, and the release of calcium ions in saline over 28 days. In contrast, impregnation of partially sintered hydroxyapatite with calcium nitrate resulted in calcium carbonate on the sintered surface. Impregnation correlated with the release of calcium ions into the saline, surface pitting, and weight loss.

Keywords

ceramicinfiltration (assembly)biomaterial
Type
Invited Articles
Information
Journal of Materials Research , Volume 32 , Issue 17: Focus Issue: Achieving Superior Ceramics and Coating Properties through Innovative Processing , 14 September 2017 , pp. 3260 - 3270
Check for updates
Copyright
Copyright © Materials Research Society 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Contributing Editor: Eugene Medvedovski

References

REFERENCES

Praemer, A., Furner, S., and Rice, D.P.: Musculoskeletal Conditions in the United States (The American Academy of Orthopaedic Surgeons, Park Ridge, IL, 1992); p. 85.Google Scholar
Einhorn, T.A.: Enhancement of fracture-healing. J. Bone Jt. Surg., Am. Vol. 77, 940 (1995).Google Scholar
Keivyon, K.R. and Tseng, S.C.: Limbal autograft transplantation for ocular surface disorders. Ophthalmology 96(5), 709 (1989).Google Scholar
Trulock, E.P., Christie, J.D., Edwards, L.B., Boucek, M.M., Aurora, P., Taylor, D.O., Dobbels, F., Rahmel, A.O., Keck, B.M., and Hertz, M.I.: Registry of the international society for heart and lung transplantation: Twenty-fourth official adult lung and heart-lung transplantation report-2007. J. Heart Lung Transplant. 26(8), 782 (2007).Google Scholar
Bladé, J., Samson, D., Reece, D., Apperley, J., Björkstrand, B., Gahrton, G., Gertz, M., Giralt, S., Jagannath, S., and Vesole, D.: Criteria for evaluating disease response and progression in patients with multiple myeloma treated by high-dose therapy and haemopoietic stem cell transplantation. Myeloma Subcommittee of the EBMT. European Group for Blood and Marrow Transplant. Br. J. Haematol. 102(5), 1115 (1998).Google Scholar
Pavletic, S.Z., Khouri, I.F., Haagenson, M., King, R.J., Bierman, P.J., Bishop, M.R., Carston, M., Giralt, S., Molina, A., Copelan, E.A., Ringdén, O., Roy, V., Ballen, K., Adkins, D.R., McCarthy, P., Weisdorf, D., Montserrat, E., and Anasetti, C.: Unrelated donor marrow transplantation for B-cell chronic lymphocytic leukemia after using myeloablative conditioning: Results from the center for International blood and marrow transplant research. J. Clin. Oncol. 23(24), 5788 (2005).CrossRefGoogle ScholarPubMed
Thaler, M.S., Klausner, R.D., and Cohen, H.J.: Medical Immunology (Lippincott, Philadelphia and Toronto, 1977).Google Scholar
Oostendorp, R.A.J., Harvey, K.N., Kusadasi, N., de Bruijn, M.F.T.R., Saris, C., Ploemacher, R.E., Medvinsky, A.L., and Dzierzak, E.A.: Stromal cell lines from mouse aorta-gonads-mesonephros subregions are potent supporters of hematopoietic stem cell activity. Blood 99, 1183 (2002).Google Scholar
Taichman, R.S. and Emerson, S.G.: The role of osteoblasts in the hematopoietic microenvironment. Stem Cells 16, 7 (1998).Google Scholar
Visnjic, D., Kalajzic, Z., Rowe, D.W., Katavic, V., Lorenzo, J., and Aguila, H.L.: Hematopoiesis is severely altered in mice with an induced osteoblast deficiency. Blood 103, 3258 (2004).Google Scholar
Visnjic, D., Kalajzic, I., Gronowicz, G., Aguila, H.L., Clark, S.H., Lichtler, A.C., and Rowe, D.W.: Conditional ablation of the osteoblast lineage in Col2.3Δk transgenic mice. J. Bone Miner. Res. 16, 2222 (2001).CrossRefGoogle ScholarPubMed
Wilson, A. and Trumpp, A.: Bone-marrow haematopoietic-stem-cell niches. Nat. Rev. Immunol. 6, 93 (2006).Google Scholar
Fuchs, E., Tumbar, T., and Guasch, G.: Socializing with the neighbors: Stem cells and their niche. Cell 116, 769 (2004).Google Scholar
Jing, D., Fonseca, A.V., Alakel, N., Fierro, F.A., Muller, K., Bornhauser, M., Ehninger, G., Corbeil, D., and Ordemann, R.: Hematopoietic stem cells in co-culture with mesenchymal stromal cells—Modeling the niche compartments. Haematologica 95, 542 (2010).Google Scholar
Habibovic, P. and de Groot, K.: Osteoinductive biomaterials—Properties and relevance in bone repair. J. Tissue Eng. Regener. Med. 1(1), 25 (2007).Google Scholar
Gauthiera, O., Müllerb, R., von Stechowb, D., Lamy, B., Weiss, P., Bouler, J.M., Aguado, E., and Daculsi, G.: In vivo bone regeneration with injectable calcium phosphate biomaterial: A three-dimensional micro-computed tomographic, biomechanical and SEM study. Biomaterials 26(27), 5444 (2005).Google Scholar
Heughebaert, M., LeGeros, R.Z., Gineste, M., Guilhem, A., and Bonel, G.: Physicochemical characterization of deposits associated with HA ceramics implanted in nonosseous sites. J. Biomed. Mater. Res. 22, 257 (1988).Google Scholar
Daculsi, G.: Biphasic calcium phosphate concept applied to artificial bone, implant coating and injectable bone substitute. Biomaterials 19(16), 1473 (1998).CrossRefGoogle ScholarPubMed
Schmelzer, E., Finoli, A., Nettleship, I., and Gerlach, J.: Long-term three-dimensional perfusion culture of human adult bone marrow mononuclear cells in bioreactors. Biotechnol. Bioeng. 112, 801 (2015).Google Scholar
Shang, Q., Wang, Z., Liu, W., Shi, Y., Cui, L., and Cao, Y.: Tissue-engineered bone repair of Sheep cranial defects with autologous bone marrow stromal cells. J. Craniofac. Surg. 12(6), 586 (2001).Google Scholar
Zhu, L., Liu, W., Cui, L., and Cao, Y.: Tissue-engineered bone repair of goat-femur defects with osteogenically induced bone marrow stromal cells’. Tissue Eng. 12(3), 423 (2006).CrossRefGoogle ScholarPubMed
Imaizumi, H., Sakurai, M., Kashimoto, O., Kikawa, T., and Suzuki, O.: Comparative study on osteoconductivity by synthetic octacalcium phosphate and sintered hydroxyapatite in rabbit bone marrow. Calcif. Tissue Int. 78(1), 45 (2006).Google Scholar
Marra, K.G., Szem, J.W., Kumta, P.N., DiMilla, P.A., and Weiss, L.E.: In vitro analysis of biodegradable polymer blend/hydroxyapatite composites for bone tissue engineering. J. Biomed. Mater. Res. 47(3), 324 (1999).3.0.CO;2-Y>CrossRefGoogle ScholarPubMed
Scadden, D.T.: The stem-cell niche as an entity of action. Nature 441(7097), 1075 (2006).Google Scholar
Adams, G.B., Chabner, K.T., Alley, I.R., Olson, D.P., Szczepiorkowski, Z.M., Poznansky, M.C., Kos, C.H., Pollak, M.R., Brown, E.M., and Scadden, D.T.: Stem cell engraftment at the endosteal niche is specified by the calcium-sensing receptor. Nature 439, 599 (2006).Google Scholar
Nakamura, S., Matsumoto, T., Sasaki, J.I., Egusa, H., Lee, K.Y., Nakano, T., Sohmura, T., and Nakahira, A.: Effect of calcium ion concentrations on osteogenic differentiation and hematopoietic stem cell niche-related protein expression in osteoblasts. Tissue Eng., Part A 16(8), 2467 (2010).Google Scholar
Chakraborty, S., Bag, S., Pal, S., and Mukherjee, A.K.: Structural and microstructural characterization of bioapatites and synthetic hydroxyapatite using X-ray powder diffraction and Fourier transform infrared techniques. J. Appl. Crystallogr. 39, 385 (2006).Google Scholar
Barg, S., Soltmann, C., Andrade, M., Koch, D., and Grathwohl, G.: Cellular ceramics by direct foaming of emulsified ceramic powder suspensions. J. Am. Ceram. Soc. 91, 2823 (2008).Google Scholar
Chatterji, S.: Mechanism of expansion of concrete due to the presence of dead-burnt CaO and MgO. Cem. Concr. Res. 25(1), 51 (1995).CrossRefGoogle Scholar
López-Arce, P., Gómez-Villalba, L.S., Martínez-Ramírez, S., Álvarez de Buergo, M., and Forta, R.: Influence of relative humidity on the carbonation of calcium hydroxide nanoparticles and the formation of calcium carbonate polymorphs. Powder Technol. 205(1–3), 263 (2011).Google Scholar
Moseke, C. and Gbureck, U.: Tetracalcium phosphate: Synthesis, properties and biomedical applications. Acta Biomater. 6(10), 3815 (2010).Google Scholar
Zhang, Q., Schmelzer, E., Gerlach, J.C., and Nettleship, I.: A microstructural study of the degradation and calcium release from hydroxyapatite-calcium oxide ceramics made by infiltration. Mater. Sci. Eng., C (Online) (2016). doi.org/10.1016/j.msec.2016.11.064.Google Scholar
Liao, J., Duan, X., Li, Y., Zheng, C., Yang, Z., Zhou, A., and Zou, D.: Synthesis and mechanism of tetracalcium phosphate from nanocrystalline precursor. J. Nanomater. 2014, 11 (2014).Google Scholar
Carayon, M.T. and Lacout, J.L.: Study of the Ca/P atomic ratio of the amorphous phase in plasma-sprayed hydroxyapatite coatings. J. Solid State Chem. 172(2), 339 (2003).Google Scholar
Gbureck, U., Barralet, J.E., Hofmann, M., and Thull, R.: Mechanical activation of tetracalcium phosphate. J. Am. Ceram. Soc. 87, 311 (2004).Google Scholar
Jalota, S., Tas, A.C., and Bhaduri, S.B.: Synthesis of HA-seeded TTCP (Ca4(PO4)2O) powders at 1230 °C from Ca(CH3COO)2·H2O and NH4H2PO4 . J. Am. Ceram. Soc. 88, 3353 (2005).CrossRefGoogle Scholar
Romeo, H.E. and Fanovich, M.A.: Synthesis of tetracalcium phosphate from mechanochemically activated reactants and assessment as a component of bone cements. J. Mater. Sci.: Mater. Med. 19, 2751 (2008).Google Scholar
Deng, M., Hong, D., Lan, X., and Tang, M.: Mechanism of expansion in hardened cement pastes with hard-burnt free lime. Cem. Concr. Res. 25(2), 440 (1995).Google Scholar
Gbureck, U., Hofmann, M.P., and Barralet, J.E.: Thermal performance of mechanically activated tetracalcium phosphate. J. Am. Ceram. Soc. 88, 1327 (2005).CrossRefGoogle Scholar