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Stimuli-responsive liquid crystal elastomers for dynamic cell culture

Published online by Cambridge University Press:  10 February 2015

Aditya Agrawal ,
Oluwatomiyin Adetiba ,
Hojin Kim ,
Huiying Chen ,
Jeffrey G. Jacot  and
Rafael Verduzco
Aditya Agrawal
Affiliation:
Department of Chemical and Biomolecular Engineering, William Marsh Rice University, Houston, Texas 77005, USA
Oluwatomiyin Adetiba
Affiliation:
Department of Bioengineering, William Marsh Rice University, Houston, Texas 77005, USA
Hojin Kim
Affiliation:
Department of Chemical and Biomolecular Engineering, William Marsh Rice University, Houston, Texas 77005, USA
Huiying Chen
Affiliation:
Department of Bioengineering, William Marsh Rice University, Houston, Texas 77005, USA
Jeffrey G. Jacot*
Affiliation:
Department of Bioengineering, William Marsh Rice University, Houston, Texas 77005, USA; and Division of Congenital Heart Surgery, Texas Children's Hospital, Congenital Heart Surgery Services, Houston, Texas 77030, USA
Rafael Verduzco*
Affiliation:
Department of Chemical and Biomolecular Engineering, William Marsh Rice University, Houston, Texas 77005, USA
*
b)e-mail: jeff.jacot@rice.edu
a)Address all correspondence to these authors. e-mail: rafaelv@rice.edu
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Abstract

Responsive, biocompatible substrates are of interest for directing the maturation and function of cells in vitro during cell culture. This can potentially provide cells and tissues with desirable properties for regenerative therapies. Here, we demonstrate a straightforward and scalable approach to attach, align, and dynamically load cardiomyocytes on responsive liquid crystal elastomer (LCE) substrates. Monodomain LCEs exhibit reversible shape changes in response to cyclic heating, and when immersed in an aqueous medium on top of resistive heaters, shape changes are fast, reversible, and produce minimal temperature changes in the surroundings. We systematically characterized the strain response of LCEs in water and demonstrated the attachment and alignment of neonatal rat ventricular myocytes on LCE substrates. Cardiomyocytes attached to both static and stimulated LCE substrates, and under cyclic stimulation, cardiomyocytes aligned along the primary direction of strain. This work demonstrates the potential of LCEs as stimuli-responsive substrates for dynamic cell culture.

Keywords

biomedicalbiomaterialliquid crystal
Type
Invited Feature Papers
Information
Journal of Materials Research , Volume 30 , Issue 4 , 28 February 2015 , pp. 453 - 462
Copyright
Copyright © Materials Research Society 2015 

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