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Study on cytotoxicity of polyethylene glycol and albumin bovine serum molecule–modified quantum dots prepared by hydrothermal method

Published online by Cambridge University Press:  17 April 2020

Enlv Hong ,
Lumin Liu ,
Chen Li ,
Dan Shan ,
Hailong Cao  and
Baiqi Wang
Enlv Hong
Affiliation:
Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Tianjin 300070, China
Lumin Liu
Affiliation:
Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Tianjin 300070, China
Chen Li
Affiliation:
Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Tianjin 300070, China
Dan Shan
Affiliation:
Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Tianjin 300070, China
Hailong Cao*
Affiliation:
Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin 300052, China
Baiqi Wang*
Affiliation:
Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Tianjin 300070, China Tianjin Institute of Digestive Disease, Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin 300070, China National Demonstration Center for Experimental Preventive, Medicine Education (Tianjin Medical University), Tianjin 300070, China
*
*)Address all correspondence to these authors. e-mail: Cao HL: caohailong@tmu.edu.cn and Wang BQ: wbqpaper@126.com
*)Address all correspondence to these authors. e-mail: Cao HL: caohailong@tmu.edu.cn and Wang BQ: wbqpaper@126.com
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Abstract

Fluorescent quantum dots (QDs) modified with polyethylene glycol (PEG) and albumin bovine serum (BSA) have profound application in the detection and treatment of hepatocellular carcinoma (HCC) cells. In the present study, the effects and mechanism of PEG and BSA modification on the cytotoxicity of QDs have been explored. It was found that the diameter of the as-prepared QDs, PEG@QDs, BSA@QDs is 3–5 nm, 4–5 nm, and 4–6 nm, respectively. With increase of the treatment time from 0 to 24 h, the HCC cell viability treated with QDs, PEG@QDs, and BSA@QDs obviously decreases, showing a certain time-dependent manner. When the concentration of several nanomaterials is increased from 10 to 90 nM, the cell viability decreases accordingly, exhibiting a certain concentration-dependent manner. Under the same concentration change conditions, the reactive oxygen species contents of cells treated by QDs, PEG@QDs, and BSA@QDs also rise from 7.9 × 103, 6.7 × 103, and 4.7 × 103 to 13.2 × 103, 14.3 × 103, and 12.3 × 103, respectively. In these processes, superoxide dismutase does not play a major role. This study provides strong foundation and useful guidance for QD applications in the diagnosis and treatment of HCC.

Keywords

quantum dotssurface modificationhepatocellular carcinomascell activityreactive oxygen species
Type
Article
Information
Journal of Materials Research , Volume 35 , Issue 9 , 14 May 2020 , pp. 1135 - 1142
Copyright
Copyright © Materials Research Society 2020

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Footnotes

#)

These authors contributed equally to this work.

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