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Tunable glass reference materials for quantitative backscattered electron imaging of mineralized tissues

Published online by Cambridge University Press:  21 August 2012

Sara E. Campbell ,
Roy H. Geiss ,
Steve A. Feller  and
Virginia L. Ferguson
Sara E. Campbell
Affiliation:
Department of Mechanical Engineering, University of Colorado, Boulder, Colorado 80309; and Materials Reliability Division, NIST, Boulder, Colorado80305
Roy H. Geiss
Affiliation:
Materials Reliability Division, NIST, Boulder, Colorado80305
Steve A. Feller
Affiliation:
Physics Department, Coe College, Cedar Rapids, Iowa52402
Virginia L. Ferguson*
Affiliation:
Department of Mechanical Engineering, University of Colorado, Boulder, Colorado80309
*
a)Address all correspondence to this author. e-mail: virginia.ferguson@colorado.edu
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Abstract

Backscattered electron microscopy provides gray-level contrast resulting from variations in atomic composition. Through the use of reference materials, quantitative backscattered electron (qBSE) imaging can be used to measure the mineral content of mineralized tissues at submicron resolution. We have developed novel tunable reference materials that can be adjusted for analysis of an individual tissue or a wide range of tissues with variable atomic density. As an alternative to conventional metallic reference materials, these amorphous materials maintain long-term stability and possess no long-range order that may induce channeling contrast. Using these reference materials, we characterized the mineral content of a broad range of mineralized tissues from immature mouse femur to whale bulla. Mineral volume fraction correlated to more traditional measurements of mineral content with microcomputed tomography and ashing techniques. Further, we demonstrate the advantage of location-matched measurements of nanomechanical properties and qBSE mineral content.

Keywords

bonenanoindentationscanning electron microscopy
Type
Articles
Information
Journal of Materials Research , Volume 27 , Issue 19 , 14 October 2012 , pp. 2568 - 2577
Copyright
Copyright © Materials Research Society 2012

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