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Design and Simulation of Continuous Dielectrophoretic Flow Sorters

Published online by Cambridge University Press:  05 May 2011

T.-S. Leu ,
H.-Y. Chen  and
F-B. Hsiao
T.-S. Leu*
Affiliation:
Department of Aeronautics and Astronautics, National Cheng Kung University, Tainan, Taiwan 70101, R.O.C.
H.-Y. Chen*
Affiliation:
Department of Aeronautics and Astronautics, National Cheng Kung University, Tainan, Taiwan 70101, R.O.C.
F-B. Hsiao*
Affiliation:
Department of Aeronautics and Astronautics, National Cheng Kung University, Tainan, Taiwan 70101, R.O.C.
*
*Assistant Professor
**Ph.D. candidate
***Professor
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Abstract

This paper was an attempt to investigate, through numerical simulation, the designs of DEP flow sorters when applied with different ratios of the electrodes to generate different electrical fields, and to explore the sorting capability of the flow sorters, defined as the degree of particle deflection, under different operation of parameters.

In order to obtain the maximal DEP negative particle deflection, which was believed as an indicator of greater sorting capability, we have investigated different non-uniform electrical fields produced by combinations of electrodes with different length of two poles, ranging from 1:2 up to 1:9. The finding of numerical simulation indicated that the length ratio 1:3 of the electrode poles produced the electrical fields that maximized the particle deflection.

Moreover, different parameters of applied voltage, flow rate, particle diameters, and distance between two electrical poles were designed to investigate their effects on particle deflection of flow sorters. The numerical simulation of the study showed that the DEP flow sorter was demonstrated as a linear system with respect to the applied voltage and particle diameter. In this study, we tried to operationally define flow rate as the time duration while the flow passed the electrical fields, and thus investigated how particle deflect with the different time given. We found that the particle deflected more when the flow was allowed with longer time to pass the electrical fields. The study also showed that the distance the particles deflect from the centerline is in inverse proportion to the square distance between the two electrical poles.

Keywords

DielectrophoreticSorters.
Type
Articles
Information
Journal of Mechanics , Volume 22 , Issue 2 , June 2006 , pp. 99 - 106
Copyright
Copyright © The Society of Theoretical and Applied Mechanics, R.O.C. 2006

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References

REFERENCES

1.Bonner, W. A., Hulett, H. R. and Sweet, R. G., “Fluorescence Activated Cell Sorting,” Rev. Sci. Instrument, 43, pp. 404–409 (1972).CrossRefGoogle ScholarPubMed
2.Telleman, P., Larsen, U. D., Kutter, J., Friis, P. and Wolff, A., “Micro Tools for Cell Handling,” Proceedings in SPIE Conference on Microfluidic Devices and Systems (2000).CrossRefGoogle Scholar
3.Anne, Y. Fu., Spence, Charles., Scherer, Axel, Arnold, Frances H. and Quake, Stephen R., “A Microfabricated Fluorescene-Activated Cell Sorter,” Nano Biotechnology, 17(11), pp. 1109–1111 (1999).Google Scholar
4.Telleman, P., Larsen, U. D., Philip, J., Blankenstein, G. and Wolff, A., “Cell Sorting in Mcrofluidic Systems,” Proc. Micro Total Analysis Systems ‘98, Banff, pp. 39–44 (1998).CrossRefGoogle Scholar
5.Grover, S. C., Skirtach, A. G., Gauthier, R. C. and Grover, C. P., “Automated Single-Cell Sorting System Based on Optical Trapping,” Journal of Biomedical Optics, 6(1), pp. 14–22 (2001).CrossRefGoogle ScholarPubMed
6.Ohta, A. T., Chiou, P.-Y. and Wu, M. C., “Dynamic Array Manipulation of Microscopic Particles via Optoelectronics Tweezers,” Hilton Head Workshop (2004).Google Scholar
7.Larsen, U. D., Blankenstein, G. and Branebjerg, J., “Flow Switch for Analyte Injection and Cell/Particle Sorting,” Proc. Micro Total Analysis Systems ‘96, Basel, pp. 113–115 (1996).Google Scholar
8.Lee, G.-B., Hung, C.-L., Ke, B.-J., Huang, G.-R. and Hwei, B.-H., “Micromachined Pre-Focused lxN Flow Switch,” Journal of Micromechanics and Microengineering, 11, pp. 567–573 (2001)CrossRefGoogle Scholar
9.Huh, D., Tung, Y.-C., Grotberg, J. B., Skerlos, S., Ku-rabayashi, K. and Takayama, S., “Air-Liquid Two-Phase Microfluidic System for Low-Cost, Low-Volume and Low-Power Micro Flow Cytometer,” Proc. Micro Total Analysis Systems ‘01, Monterey, pp. 468–470 (2001).CrossRefGoogle Scholar
10.Tashoro, K., Sekiguchi, T., Shoji, S., Funatsu, T., Masumoto, W. and Sato, H., “Design and Simulation of Particles and Biomolecules Handling Micro Flow Cells with Tree-Dimensional Sheath Flow,” Proc. Micro Total Analysis Systems, Enschede, pp. 209–212 (2000).Google Scholar
11.Ichiki, T., Ujiie, T., Hara, T., Horiike, Y. and Yasuda, K., “On-Chip Cell Sorter for Single Cell Expression Analysis,” Proc. Micro Total Analysis Systems ‘01, Monterey, pp. 271–273 (2001).CrossRefGoogle Scholar
12.Takahashi, K., Hattori, A., Suzuki, I., Ichiki, T. and Yasuda, K., “Non-destructive On-Chip Cell Sorting System with Real-Time Microscopic Image Processing,” Journal of Nanobiotechnology (2004).CrossRefGoogle ScholarPubMed
13.Pohl, H. A., Dielectrophoresis, Cambridge University Press (1978).Google Scholar
14.Jones, T. B., Electromechanics of Particles, Cambridge University Press (1995).CrossRefGoogle Scholar
15.Yuan, S. W., Foundations of Fluid Mechanics, Prentice-Hall, Inc. (1982).Google Scholar