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Mechanics for the Adhesion and Aggregation of Red Blood Cells on Chitosan

Published online by Cambridge University Press:  08 August 2018

K. Y. Chen ,
T. H. Lin ,
C. Y. Yang ,
Y. W. Kuo  and
U. Lei
K. Y. Chen
Affiliation:
Institute of Applied MechanicsNational Taiwan UniversityTaipei, Taiwan
T. H. Lin
Affiliation:
Department of TraumatologyNational Taiwan University HospitalTaipei, Taiwan
C. Y. Yang
Affiliation:
Department of Mechanical and Automatic EngineeringChung Chou University of Science and TechnologyChanghua, Taiwan
Y. W. Kuo
Affiliation:
Department of Regulatory and R & DCoreLeader Biotech Co.New Taipei City, Taiwan
U. Lei*
Affiliation:
Institute of Applied MechanicsNational Taiwan UniversityTaipei, Taiwan
*
*Corresponding author (leiu@iam.ntu.edu.tw)
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Abstract

Hemostasis, a process which causes bleeding to stop, can be enhanced using chitosan; but the detailed mechanism is unclear. Red blood cells (RBCs) adhere to chitosan because of their opposite charges, but the adhesion force is small, 3.83 pN as measured here using an optical tweezer, such that the direct adhesion cannot be the sole cause for hemostasis. However, it was observed in this study that layer structures of aggregated RBCs were formed next to chitosan objects in both static and flowing environments, but not formed next to cotton and rayon yarns. The layer structure is the clue for the initiation of hemostatsis. Through the supporting measurements of zeta potentials of RBCs and pH's using blood-chitosan mixtures, it is proposed here that the formation of the RBC layer structure next to chitosan objects is due to the reduction of repulsive electric double layer force between RBCs, because of the association of H+ deprotonated from chitosan with COO− on RBC membrane, under the DLVO (Derjaguin-Landau-Verwey-Overbeek) theory. The results are beneficial for designing effective chitosan-based wound dressings, and also for general biomedical applications.

Keywords

Cell adhesionCell aggregationChitosanMechanics
Type
Research Article
Information
Journal of Mechanics , Volume 34 , Issue 5 , October 2018 , pp. 725 - 732
Copyright
Copyright © The Society of Theoretical and Applied Mechanics 2018 

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