Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-10T10:16:20.170Z Has data issue: false hasContentIssue false
  • English
  • Français

Mechanics for the Adhesion and Aggregation of Red Blood Cells on Chitosan

Published online by Cambridge University Press:  08 August 2018

K. Y. Chen ,
T. H. Lin ,
C. Y. Yang ,
Y. W. Kuo  and
U. Lei
K. Y. Chen
Affiliation:
Institute of Applied MechanicsNational Taiwan UniversityTaipei, Taiwan
T. H. Lin
Affiliation:
Department of TraumatologyNational Taiwan University HospitalTaipei, Taiwan
C. Y. Yang
Affiliation:
Department of Mechanical and Automatic EngineeringChung Chou University of Science and TechnologyChanghua, Taiwan
Y. W. Kuo
Affiliation:
Department of Regulatory and R & DCoreLeader Biotech Co.New Taipei City, Taiwan
U. Lei*
Affiliation:
Institute of Applied MechanicsNational Taiwan UniversityTaipei, Taiwan
*
*Corresponding author (leiu@iam.ntu.edu.tw)
Get access

Abstract

Hemostasis, a process which causes bleeding to stop, can be enhanced using chitosan; but the detailed mechanism is unclear. Red blood cells (RBCs) adhere to chitosan because of their opposite charges, but the adhesion force is small, 3.83 pN as measured here using an optical tweezer, such that the direct adhesion cannot be the sole cause for hemostasis. However, it was observed in this study that layer structures of aggregated RBCs were formed next to chitosan objects in both static and flowing environments, but not formed next to cotton and rayon yarns. The layer structure is the clue for the initiation of hemostatsis. Through the supporting measurements of zeta potentials of RBCs and pH's using blood-chitosan mixtures, it is proposed here that the formation of the RBC layer structure next to chitosan objects is due to the reduction of repulsive electric double layer force between RBCs, because of the association of H+ deprotonated from chitosan with COO− on RBC membrane, under the DLVO (Derjaguin-Landau-Verwey-Overbeek) theory. The results are beneficial for designing effective chitosan-based wound dressings, and also for general biomedical applications.

Keywords

Cell adhesionCell aggregationChitosanMechanics
Type
Research Article
Information
Journal of Mechanics , Volume 34 , Issue 5 , October 2018 , pp. 725 - 732
Copyright
Copyright © The Society of Theoretical and Applied Mechanics 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Pillai, C. K. S., Paul, W. and Sharma, C. P., “Chitin and Chitosan Polymers: Chemistry, Solubility and Fiber Formation,” Progress in Polymer Science, 34, pp. 641–678 (2009).Google Scholar
2. Dash, M., Chiellini, F., Ottenbrite, R. M. and Chiellini, E., “A Versatile Semi-Synthetic Polymer in Biomedical Applications,” Progress in Polymer Science, 36, pp. 981–1014 (2001).Google Scholar
3. Yi, H. et al., “Biofabrication with Chitosan,” Biomacromolecules, 6, pp. 2881–2894 (2005).Google Scholar
4. Dai, T., Tanaka, M., Huang, Y. Y. and Hamblin, M. R., “Chitosan Preparations for Wounds and Burns: Antimicrobial and Wound-Healing Effects,” Expert Review of Anti-Infective Therapy, 9, pp. 857–879 (2011).Google Scholar
5. Jayakumar, R., Prabaharan, M., Sudheesh Kumar, P. T., Nair, C. V. and Tamura, H., “Biomaterials Based on Chitin and Chitosan in Wound Dressing Applications,” Biotechnology Advances, 29, pp. 322–337 (2011).Google Scholar
6. Bennett, B. L. et al., “Management of External Hemorrhage in Tactical Combat Casualty Care: Chitosan-Based Hemostatic Gauze Dressings. TCCC Guidelines – Change 13-05,” Journal of Special Operations Medicine, 14, pp. 40–57 (2014).Google Scholar
7. Pusateri, A. E. et al., “Making Sense of the Preclinical Literature on Advanced Hemostatic Products,” The Journal of Trauma: Injury, Infection, and Critical Care, 60, pp. 674–682 (2006).Google Scholar
8. Gordy, S. D., Rhee, P. and Schreiber, M. A., “Military Applications of Novel Hemostatic Devices,” Expert Review of Medical Devices, 8, pp. 41–47 (2011).Google Scholar
9. Brown, M. A., Daya, M. R. and Worley, J. A., “Experience with Chitosan Dressings in a Civilian EMS System,” The Journal of Emergency Medicine, 37, pp. 1–7 (2009).Google Scholar
10. Mlekusch, W. et al., “Arterial Puncture Site Management after Percutaneous Transluminal Procedures Using a Hemostatic Wound Dressing (Clo-Sur P.A.D.) Versus Conventional Manual Compression: a Randomized Controlled Trial,” Journal of Endovascular Therapy, 13, pp. 23–31 (2006).Google Scholar
11. Thatte, H. S., Zagarins, S., Khuri, S. F. and Fischer, T. H., “Mechanisms of Poly-N-Acetyl Glucosamine Polymer-Mediated Hemostasis: Platelet Interactions,” The Journal of Trauma: Injury, Infection, and Critical Care, 57, pp. S13-S21 (2004).Google Scholar
12. Chou, T. C., Fu, E., Wu, C. J. and Yeh, J. H., “Chitosan Enhances Platelet Adhesion and Aggregation,” Biochemical and Biophysical Research Communications, 302, pp. 480–483 (2003).Google Scholar
13. Rao, S. B. and Sharma, C. P., “Use of Chitosan as a Biomaterial: Studies on Its Safety and Hemostatic Potential,” Journal of Biomedical Materials Research, 34, pp. 21–28 (1997).Google Scholar
14. Millner, R., Lockhart, A. S. and Marr, R., “Chitosan Arrests Bleeding in Major Hepatic Injuries with Clotting Ddysfunction: an in vivo Experimental Study in a Model of Hepatic Injury in the Presence of Moderate Systemic Heparinisation,” Annals of the Royal College of Surgeons of England, 92, pp. 559–561 (2010).Google Scholar
15. Chan, L. W. et al., “PolySTAT-Modified Chitosan Gauzes for Improved Hemostasis in External Hemorrhage,” Acta Biomaterialia, 31, pp. 178–185 (2016).Google Scholar
16. Lo, Y. J. et al., “Derivation of the Cell Dielectric Properties Based on Clausius-Mossotti Factor,” Applied Physics Letters, 104, pp. 113702 (2014).Google Scholar
17. Lei, U. et al., “A Travelling Wave Dielectrophoretic Pump for Blood Delivery,” Lab on a Chip, 9, pp. 1349–1356 (2009).Google Scholar
18. Sagvolden, G., Giaever, I., Pettersen, E. O. and Feder, J., “Cell Adhesion Force Microscopy,” Proceedings of the National Academy of Sciences of the United States of America, USA, 96, pp. 471–476 (1999).Google Scholar
19. Fontes, A. et al., “Measuring Electrical and Mechanical Properties of Red Blood Cells with Double Optical Tweezers,” Journal of Biomedical Optics, 13, pp. 014001 (2008).Google Scholar
20. Kendall, K. and Roberts, A. D., “van der Waals Forces Influencing Adhesion of Cells,” Philosophical Transactions of the Royal Society B, 370, pp. 20140078 (2015).Google Scholar
21. Fernandes, H. P., Cesar, C. L. and Barjas-Castro, M. L., “Electrical Properties of the Red Blood Cell Membrane and Immunohematological Investigation,” Revista Brasileira De Hematologia E Hemoterapia, 33, pp. 297–301 (2011).Google Scholar
22. Wagner, C., Steffen, P. and Svetina, S., “Aggregation of Red Blood Cells: From Rouleaux to Clot Formation,” Comptes Rendus Physique, 14, pp. 459–469 (2013).Google Scholar
23. Israelachvilli, J. N., Intermolecular and Surface Forces, 3rd ed., Academic, London (2011).Google Scholar
24. Eylar, E. H., Madoff, M. A., Brody, O. V. and Oncley, J. L., “The Contribution of Sialic Acid to the Surface Charge of the Erythrocyte,” The Journal of Biological Chemistry, 237, pp. 1992–2000 (1962).Google Scholar
25. Jan, K. M. and Chien, S., “Role of Surface Electric Charge in Red Blood Cell Interaction,” The Journal of General Physiology, 61, pp. 638–654 (1973).Google Scholar
26. Tokumasu, F., Ostera, G. R., Amaratunga, C. and Fairhurst, R. M., “Modifications in Erythrocyte Membrane Zeta potential by Plasmodium Falciparum Infection,” Experimental Parasitology, 131, pp. 245–251 (2012).Google Scholar
27. Jan, K. M. and Chien, S., “Influence of the Ionic Composition of Fluid Medium on Red Cell Aggregation,” The Journal of General Physiology, 61, pp. 655–668 (1973).Google Scholar
28. Correlo, V. M. et al., “Water Absorption and Degradation Characteristics of Chitosan-Based Polyesters and Hydroxyapatite Composites,” Macromolecular Bioscience, 7, pp. 354–363 (2007).Google Scholar
29. Happel, J. and Brenner, H., Low Reynolds Number Hydrodynamics. Martinus Nijhoff Publishers, Boston (1986).Google Scholar