Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-10T12:47:16.787Z Has data issue: false hasContentIssue false
  • English
  • Français

A Modified Travelling Wave Dielectrophoretic Method for the Measurement of the Imaginary Part of the Clausius-Mossotti Factor Using Dual Frequency Operation

Published online by Cambridge University Press:  04 September 2014

Y.-Y. Lin ,
U. Lei ,
Y.-J. Lo ,
M.-S. Wu  and
P.-C. Yang
Y.-Y. Lin
Affiliation:
Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan
U. Lei*
Affiliation:
Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan
Y.-J. Lo
Affiliation:
Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan
M.-S. Wu
Affiliation:
Department of Internal Medicine, National Taiwan University, Taipei, Taiwan
P.-C. Yang
Affiliation:
Department of Internal Medicine, National Taiwan University, Taipei, Taiwan
*
*leiu@iam.ntu.edu.tw
Get access

Abstract

The imaginary part of the Clausius-Mossotti factor, Ki, is crucial for quantitative particle/cell manipulation and characterization using travelling wave dielectrophoresis (twDEP) and electrorotation (ER). It can be measured rather easily using twDEP instead of using ER, as numerical calculation of the electric field and complicated devices are not needed for evaluating Ki using twDEP. However, the current single frequency twDEP method works only for particles/cells exhibiting negative dielectrophoresis (DEP), which is in general the case when cells are manipulated in physiological strength buffers with conductivity of order of 1S/m. In order to remove such a restriction, a modified twDEP method is proposed using dual frequency operation here capable of measuring the Ki spectra of particles/cells for the entire range of medium conductivity, irrespectively of whether the particles/cells exhibit positive or negative DEP. The success of the modified method relies on the adequate design of force balance between various forces in a designed micro channel, which was discussed in details. The method was validated, and demonstrated by measuring Ki spectra of three human cancer cells for medium conductivity from 0.01 ~ 1.2S/m. Both the method and the cell result find biomedical and other industrial applications.

Keywords

Travelling wave dielectrophoresisElectrorotationImaginary part of the Clausius-Mossotti factorDual frequency
Type
Research Article
Information
Journal of Mechanics , Volume 30 , Issue 6 , December 2014 , pp. 651 - 660
Copyright
Copyright © The Society of Theoretical and Applied Mechanics, R.O.C. 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1.Arnold, W. M. and Zimmerman, U., “Electro-Rotation: Development of a Technique for Dielectric Measurements on Individual Cells and Particles,” Journal of Electrostatics, 21, pp. 151–191 (1998).CrossRefGoogle Scholar
2.Masuda, S., Washizu, M. and Iwadare, M., “Separation of Small Particles Suspended in Liquid by Nonuniform Traveling Field,” IEEE Transaction on Industry Application, 23, pp. 474–480 (1987).CrossRefGoogle Scholar
3.Jones, T. B., Electromechanics of Particles, Cambridge University Press, Cambridge (1995).CrossRefGoogle Scholar
4.Hagedorn, R., Fuhr, G., Müller, T. and Gimsa, J., “Traveling-Wave Dielectrophoresis of Microparticles,” Electrophoresis, 13, pp. 49–54 (1992).CrossRefGoogle ScholarPubMed
5.Hughes, M. P., Nanoelectromechanics in Engineering and Biology, CRC Press, Boca Raton, Florida (2003).Google Scholar
6.Pethig, R., “Dielectrophoresis: Status of the Theory, Technology, and Applications,” Biomicrofluidics, 4, p. 022811 (2010).CrossRefGoogle ScholarPubMed
7.Reichle, C., Müller, T., Schnelle, T. and Fuhr, G., “Electro-Rotation in Octopole Micro Cages,” Journal of Physics D: Applied Physics, 32, pp. 2128–2135 (1999).Google Scholar
8.Schnelle, T., Müller, T., Reichle, C. and Fuhr, G., “Combined Dielectrophoretic Field Cages and Laser Tweezers for Electrorotation,” Applied Physics B, 70, pp. 267–274 (2000).CrossRefGoogle Scholar
9.Schnelle, T., Hagedorn, R., Fuhr, G., Fiedler, S. and Müller, T., “Three- Dimensional Electric Field Traps for Manipulation of Cells — Calculation and Experimental Verification,” Biochimica et Biophysica Acta, 1157, pp. 127–140 (1993).Google Scholar
10.Lo, Y. J., Lin, Y. Y., Lei, U., Wu, M. S. and Yang, P. C., “Measurement of the Clausius-Mossotti Factor of Generalized Dielectrophoresis,” Applied Physics Letters, 104, p. 083701 (2014).Google Scholar
11.Morgan, H., Izquierdo, A. G., Badewell, D., Green, N. G. and Ramos, A., “The Dielectrophoretic and Travelling Wave Forces Generated by Interdigitated Electrode Arrays: Analytical Solution Using Fourier Series,” Journal of Physics D: Applied Physics, 34, pp. 1553–1561 (2001).CrossRefGoogle Scholar
12.Gimsa, J., Müller, T., Schnelle, T. and Fuhr, G., “Dielectric Spectroscopy of Single Human Erythrocytes at Physiological Ionic Strength: Dispersion of the Cytoplasm,” Biophyical Journal, 71, pp. 495–506 (1996).CrossRefGoogle ScholarPubMed
13.Pethig, R., Talary, H. S. and Lee, R. S., “Enhancing Traveling-Wave Dielectrophoresis with Signal Superposition,” IEEE Engineering in Medicine and Biology, 22, pp. 43–50 (2003).CrossRefGoogle ScholarPubMed
14.Wang, X. B., Huang, Y., Becker, F. F. and Gascoyne, P. R. C., “A Unified Theory of Dielectrophoresis and Travelling Wave Dielectrophoresis,” Journal of Physics D: Applied Physics, 27, pp. 1571–1574 (1994).Google Scholar
15.Lo, Y. J. and Lei, U., “Quasistatic Force and Torque on a Spherical Particle Under Generalized Dielectrophoresis in the Vicinity of Walls,” Applied Physics Letters, 95, p. 253701 (2009).Google Scholar
16.Yang, C. Y. and Lei, U., “Quasistatic Force and Torque on Ellipsoidal Particles Under Generalized Dielectrophoresis,” Journal of Applied Physics, 102, p. 094702 (2007).Google Scholar
17.Happel, J. and Brenner, H., Low Reynolds Number Hydrodynamics, Martinus Nijhoff Publisher, Boston (1986).Google Scholar
18.Ishikawa, A., Hanai, T. and Koizumi, N., “Dielectric Properties of Dextran Gel Sephadex G-25 Dispersed in Aqueous Phases,” Japanese Journal of Applied Physics, 21, pp. 1762–1768 (1982).Google Scholar
19.Chu, Y.-W., Yang, P.-C., Yang, S.-C., Shyu, Y.-C., Hendrix, M. J. C., Wu, R. and Wu, C.-W., “Selection of Invasive and Metastatic Subpopulations from a Human Lung Adenocarcinoma Cell Line,” American Journal of Respiratory Cell and Molecular Biology, 17, pp. 353–360 (1997).Google Scholar
20.Lo, Y. J., Lei, U., Chen, K. Y., Lin, Y. Y., Huang, C. C., Wu, M. S. and Yang, P. C., “Derivation of the Cell Dielectric Properties Based on Clausius-Mossotti Factor,” Applied Physics Letters, 104, p. 113702 (2014).Google Scholar