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A Modified Travelling Wave Dielectrophoretic Method for the Measurement of the Imaginary Part of the Clausius-Mossotti Factor Using Dual Frequency Operation

Published online by Cambridge University Press:  04 September 2014

Y.-Y. Lin ,
U. Lei ,
Y.-J. Lo ,
M.-S. Wu  and
P.-C. Yang
Y.-Y. Lin
Affiliation:
Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan
U. Lei*
Affiliation:
Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan
Y.-J. Lo
Affiliation:
Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan
M.-S. Wu
Affiliation:
Department of Internal Medicine, National Taiwan University, Taipei, Taiwan
P.-C. Yang
Affiliation:
Department of Internal Medicine, National Taiwan University, Taipei, Taiwan
*
*leiu@iam.ntu.edu.tw
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Abstract

The imaginary part of the Clausius-Mossotti factor, Ki, is crucial for quantitative particle/cell manipulation and characterization using travelling wave dielectrophoresis (twDEP) and electrorotation (ER). It can be measured rather easily using twDEP instead of using ER, as numerical calculation of the electric field and complicated devices are not needed for evaluating Ki using twDEP. However, the current single frequency twDEP method works only for particles/cells exhibiting negative dielectrophoresis (DEP), which is in general the case when cells are manipulated in physiological strength buffers with conductivity of order of 1S/m. In order to remove such a restriction, a modified twDEP method is proposed using dual frequency operation here capable of measuring the Ki spectra of particles/cells for the entire range of medium conductivity, irrespectively of whether the particles/cells exhibit positive or negative DEP. The success of the modified method relies on the adequate design of force balance between various forces in a designed micro channel, which was discussed in details. The method was validated, and demonstrated by measuring Ki spectra of three human cancer cells for medium conductivity from 0.01 ~ 1.2S/m. Both the method and the cell result find biomedical and other industrial applications.

Keywords

Travelling wave dielectrophoresisElectrorotationImaginary part of the Clausius-Mossotti factorDual frequency
Type
Research Article
Information
Journal of Mechanics , Volume 30 , Issue 6 , December 2014 , pp. 651 - 660
Copyright
Copyright © The Society of Theoretical and Applied Mechanics, R.O.C. 2014 

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