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Phytoplankton community composition in the south-eastern Black Sea determined with pigments measured by HPLC-CHEMTAX analyses and microscopy cell counts

Published online by Cambridge University Press:  14 August 2014

Ertugrul Agirbas ,
Ali Muzaffer Feyzioglu ,
Ulgen Kopuz  and
Carole A. Llewellyn
Ertugrul Agirbas*
Affiliation:
Faculty of Fisheries, Department of Marine Biology, Recep Tayyip Erdogan University, 53100 Rize, Turkey
Ali Muzaffer Feyzioglu
Affiliation:
Faculty of Marine Science and Technology, Department of Fisheries Technology, Karadeniz Technical University, 61530 Camburnu-Trabzon, Turkey
Ulgen Kopuz
Affiliation:
Faculty of Fisheries, Department of Marine Biology, Recep Tayyip Erdogan University, 53100 Rize, Turkey
Carole A. Llewellyn
Affiliation:
Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth PL1-3DH, UK
*
Correspondence should be addressed to: E. Agirbas, Faculty of Fisheries, Department of Marine Biology, Recep Tayyip Erdogan University, 53100 Rize, Turkey email: eagirbas@gmail.com
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Abstract

The phytoplankton community structure and abundance in the south-eastern Black Sea was measured from February to December 2009 using and comparing high performance liquid chromatography pigment and microscopy analyses. The phytoplankton community was characterized by diatoms, dinoflagellates and coccolithophores, as revealed by both techniques. Fucoxanthin, diadinoxanthin, peridinin and 19′-hexanoyloxyfucoxanthin were the main accessory pigments showing significant correlation with diatom-C r2 = 0.56–0.71, P < 0.05), diatom-C (r2 = 0.85–0.91, P < 0.001), dinoflagellate-C (r2 = 0.39–0.88, P < 0.05) and coccolithophore-C (r2 = 0.80–0.71, P < 0.05), respectively. Microscopy counts indicated a total of 89 species, 71% of which were dinoflagellates, 23% were diatoms and 6% other species (mainly coccolithophores). Pigment-CHEMTAX analysis also indicated the presence of pico- and nanoplankton. Phytoplankton carbon (phyto-C) concentrations were highest in the upper water column, whereas chlorophyll-a (Chl-a) showed a deep maximum. Average phyto-C was higher at the coastal station (291 ± 66 µg l−1) than at the offshore station (258 ± 35 µg l−1), not statistically different (P > 0.05). The coastal station also had higher Chl-a concentrations (0.52–3.83 µg l−1) compared to the offshore station (0.63–2.55 µg l−1), not significant (P > 0.05). Our results are consistent with other studies and indicate that the southern Black Sea is shifting towards mesotrophy with the increasing prevalence of dinoflagellates compared to diatoms.

Keywords

Black Seaphytoplanktonhigh performance liquid chromatographyCHEMTAXpigmentsphyto-C
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 95 , Issue 1 , February 2015 , pp. 35 - 52
Copyright
Copyright © Marine Biological Association of the United Kingdom 2014 

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