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Reduction of RBL–2H3 cells degranulation by nitroaromatic compounds from a Bacillus strain associated to the Amazonian sponge Metania reticulata

Published online by Cambridge University Press:  20 July 2015

Enrique E. Rozas ,
Maria A. Mendes ,
Cláudio A.O. Nascimento ,
José C.V. Rodrigues ,
Rodolpho M. Albano  and
Márcio R. Custódio
Enrique E. Rozas
Affiliation:
INCT Energia, Ambiente e Biodiversidade, Universidade do Estado do Amazonas, Manaus, AM, Brazil Departmento de Fisiologia Geral. Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil
Maria A. Mendes
Affiliation:
CEPEMA, PQI-Escola Politécnica, Universidade de São Paulo, São Paulo, SP, Brazil
Cláudio A.O. Nascimento
Affiliation:
CEPEMA, PQI-Escola Politécnica, Universidade de São Paulo, São Paulo, SP, Brazil
José C.V. Rodrigues
Affiliation:
INCT Energia, Ambiente e Biodiversidade, Universidade do Estado do Amazonas, Manaus, AM, Brazil University of Puerto Rico, Jardim Botânico Sur, San Juan, PR 00926-1118, USA
Rodolpho M. Albano
Affiliation:
Departmento de Bioquímica, IBRAG, Universidade do Estado do Rio de Janeiro, RJ, Brazil
Márcio R. Custódio*
Affiliation:
Departmento de Fisiologia Geral. Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil
*
Correspondence should be addressed to:M.R.Custódio, Departamento de Fisiologia Geral (IB–USP), Rua do Matão, travessa 14, n. 101, Cidade Universitária, São Paulo, SP CEP 05508–090, Brazil email: mcust@usp.br
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Abstract

Symbionts in sponges must interact with the host immune system, and this can be mediated by immunomodulators. As the bases of the immune system in sponges resemble those of higher metazoans, it is possible that compounds from this microbiota show similar effects in other phyla. It is also known that several antibiotics, in special macrolides, can modulate many components of the immune response and sponges and their associated microorganisms are a rich source of these compounds. Therefore, we tested the immunosuppressive capacity of antibiotic substances produced by bacterial and fungal strains isolated from the Amazon freshwater sponge Metania reticulata. Fourteen bacterial and six fungal strains were obtained from samples of M. reticulata collected in the Negro River (Amazon Central Basin region), during the dry season. These cultures were monitored for natural antimicrobial activity, and two Bacillus strains (MERETb.761 and MERETb.762) and one fungus (MERETf.010) were selected. One Bacillus strain, MERETb.762, showed strong and specific antibiosis on Staphylococcus aureus and two fractions of its extract inhibited the degranulation of RBL–2H3 cells. The predicted formulas of these fractions were C12H6N4O8 and C25H4N2O6, both corresponding to nitroaromatic compounds.

Keywords

Poriferasymbiosisantibiosisimmunosuppressants
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 96 , Issue 2: Proceedings of the 9th World Sponge Conference held in Fremantle Australia in 2013: New Frontiers in Sponge Science , March 2016 , pp. 567 - 572
Copyright
Copyright © Marine Biological Association of the United Kingdom 2015 

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