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White muscle lactate and pyruvate concentrations in rested flounder, Platichthys flesus and plaice, Pleuronectes platessaa: a re-evaluation of handling and sampling techniques

Published online by Cambridge University Press:  06 October 2009

P. J. Adcock  and
P. R. Dando
P. J. Adcock
Affiliation:
Research Unit for Comparative Animal Respiration, University of Bristol, Woodland Road, Bristol, BS8 1UG
P. R. Dando
Affiliation:
The Laboratory, Marine Biological Association, Citadel Hill, Plymouth, PL1 2PB
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Abstract

Rapid fixing of skeletal muscle by a ‘freeze-clamp’ technique results in up to a 3-fold lower lactate, a slightly higher pyruvate concentration and a 2- to 4-fold decrease in lactate/pyruvate ratio, to the lowest value yet recorded for fish muscle, when compared with the more usual method of direct immersion in liquid nitrogen. This is attributed to the faster cooling rate of freeze-clamped muscle minimizing ‘sampling anoxia’. Immobilizing fish either by anaesthetic or stunning produces no significant change in metabolite levels. It is concluded that it is relatively easy to handle quiescent flatfish, but light anaesthesia ensures no muscular activity.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 63 , Issue 4 , November 1983 , pp. 897 - 903
Copyright
Copyright © Marine Biological Association of the United Kingdom 1983

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