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Antibacterial activities and bioactive compounds of the ethyl acetate extract of the sea cucumber Holothuria forskali from Tunisian coasts

Published online by Cambridge University Press:  04 March 2020

Khaoula Telahigue ,
Ridha Ghali ,
Ezzeddine Nouiri ,
Abdelbaki Labidi  and
Tarek Hajji Open the ORCID record for Tarek Hajji [Opens in a new window]
Khaoula Telahigue
Affiliation:
University of Tunis El Manar, Faculty of Sciences of Tunis, Research unit of Physiology and Aquatic Environment, 2092Tunis, Tunisia University of Manouba, Higher Institute of Biotechnology – Sidi Thabet, Laboratory of Biotechnology and Valorization of Bio-Geo-Resources LR11ES31, Biotechpole Sidi Thabet 2020 Ariana, Tunisia
Ridha Ghali
Affiliation:
University of Tunis El Manar, Laboratory of Toxicology and Environment LR12SP07 Mahmoud Yacoub Center of Urgent Medical Assistance of Tunis, Tunisia
Ezzeddine Nouiri
Affiliation:
University of Tunis El Manar, Laboratory of Toxicology and Environment LR12SP07 Mahmoud Yacoub Center of Urgent Medical Assistance of Tunis, Tunisia
Abdelbaki Labidi
Affiliation:
Chemical Analysis Service, Ministry of Agriculture, Tunis, Tunisia
Tarek Hajji*
Affiliation:
University of Manouba, Higher Institute of Biotechnology – Sidi Thabet, Laboratory of Biotechnology and Valorization of Bio-Geo-Resources LR11ES31, Biotechpole Sidi Thabet 2020 Ariana, Tunisia
*
Author for correspondence: Tarek Hajji, E-mail: tarekhaj@gmail.com; tarek.hajji@isbst.u-manouba.tn
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Abstract

Previous scientific studies have listed chemical compounds from sea cucumber species. The ability to produce bioactive metabolites derives from their response to various ecological stresses and avoidance of predation. In view of this, the present work aimed to evaluate the antibacterial activity and quantitatively assess the active components of different organs of the sea cucumber Holothuria foskali collected from the north-east of Tunisia. The antibacterial activity of ethyl acetate extracts was tested against Bacillus subtilis, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. It was revealed that Escherichia coli and Bacillus subtilis were inhibited by the sea cucumber extracts. To identify the composition of these extracts, Gas Chromatography-Mass Spectroscopy (GC-MS) and Liquid Chromatography-Mass Spectroscopy (LC-MS) methods were used. The GC-MS revealed the presence of 25 major components, the most concentrated ones were characterized as C26H27N5O6 Morphinan-4,5-epoxy-3,6-diol, 6 amino;C17H31F3O2 3-trifluoroacetoxypentadecane; C31H50O2 Stigmasta-5,22-dien-3-ol,acetate; C11H14O2 1-Phthalanol,1,3,3-trimethyl 1-phenyl; C30H48O2 Ergosta-5,22-dien-3-ol,acetate and C25H40O2 (9E)-8-Phenyl-9-octadecenoic acid methyl ester; while the LC-MS revealed the presence of eight molecules, identified as phenolic acid, phytosterol and fat-soluble vitamins. Some molecules are interesting and deserve to be explored for their potential uses as ingredients in functional foods or as raw materials for new drug forms.

Keywords

Antibacterialbiomoleculesethyl acetateextractGC-MSHolothuria forskaliLC-MS
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 100 , Issue 2 , March 2020 , pp. 229 - 237
Copyright
Copyright © Marine Biological Association of the United Kingdom 2020

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