Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-10T09:52:37.120Z Has data issue: false hasContentIssue false
  • English
  • Français

Antioxidant enzymes in sea cucumber Apostichopus japonicus (Selenka) during aestivation

Published online by Cambridge University Press:  05 July 2010

Wang Fangyu ,
Yang Hongsheng Yang ,
Wang Xiaoyu ,
Xing Kun  and
Gao Fei
Wang Fangyu
Affiliation:
Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China Henan Key Labaratory for Animal Immunology, Henan Academy of Agricultural Science, Zhengzhou, 450002, China
Yang Hongsheng Yang*
Affiliation:
Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China
Wang Xiaoyu
Affiliation:
Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China The Engineering Research Center of Seawater Utilization Technology, Ministry of Education, Hebei University of Technology, Tianjin, 300130, China
Xing Kun
Affiliation:
Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China Dalian Fisheries University, Dalian, 116023, China
Gao Fei
Affiliation:
Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China
*
Correspondence should be addressed to: H. Yang, Chinese Academy of Sciences, Aquacultural ecology and Environmental Sciences, Institute of Oceanology, Qingdao, 266071, People's Republic of China email: hshyang@ms.qdio.ac.cn; hshyang@126.com
Get access Rights & Permissions [Opens in a new window]

Abstract

To evaluate the effect of antioxidant defence in coelomic fluid of sea cucumber, Apostichopus japonicus in aestivation was studied in the field from July to November 2006 in Qingdao. During the sampling period, activities of superoxide dismutase and catalase increased significantly in August and November. Activities of glutathione reductase and glutathione decreased significantly in August and increased significantly in November and activities of Se-glutathione peroxidase increased significantly in August. There were no significant differences in total glutathione peroxidase. In relation to the water temperature in the field, it is known that the oxygen consumption rate dropped and antioxidant defence was enhanced in August. The structure and function of respiratory trees of A. japonicus were completely vivified as normal in November, and it is suggested that antioxidant defence was enhanced because of the sharp change of oxygen consumption. Data indicate that both enzymatic and metabolite antioxidant defences in sea cucumber are adaptable systems that are modulated during pre-aestivating stage and arousing stage.

Keywords

antioxidant defenceaestivationApostichopus japonicus
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 91 , Issue 1 , February 2011 , pp. 209 - 214
Copyright
Copyright © Marine Biological Association of the United Kingdom 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abe, A.S. (1995) Estivation in South American amphibians and reptiles. Brazilian Journal of Medical and Biological Research 28, 1241–1247.Google ScholarPubMed
Ahmad, S. and Pardini, R.S. (1988) Evidence for the presence of glutathione peroxidase activity towards an organic hydroperoxide in larvae of the cabbage looper moth, Trichoplusia ni. Insect Biochemistry 18, 861–866.Google Scholar
Barnhart, M.C. and McMahon, B.R. (1987) Discontinuous carbon dioxide release and metabolic depression in dormant land snails. Journal of Experimental Biology 128, 123–138.Google Scholar
Beckman, K.B. and Ames, B.N. (1997) Oxidative decay of DNA. Journal of Biological Chemistry 272, 9633–9636.CrossRefGoogle ScholarPubMed
Bicego-Nahas, K.C., Gargaglioni, L.H. and Branco, L.G.S. (2001) Seasonal changes in the preferred body temperature, cardiovascular, and respiratory responses to hypoxia in the toad, Bufo paracnemis. Comparative Physiology and Biochemistry 289, 359–365.Google ScholarPubMed
Byrne, M. (1986) The case for seasonal evisceration in the holothuroid Eupentacta quinquesemita (Selenka), a reply to Fankboner and Cameron (1985). Canadian Journal of Zoology 64, 2391–2392.Google Scholar
Choe, S. and Ohshima, Y. (1961) On the morphological and ecological differences between tow commercial forms. ‘Green’ and ‘Red’, of the Japanese common sea cucumber., Stichopus japonicus Selenka. Nippon Suyisan Gakkaishi 27, 97–106.Google Scholar
Choe, S. (1963) Study of sea cucumber, morphology, ecology and propagation of sea cucumber. Tokyo: Kaibundo Publishing House.Google Scholar
Demple, B. (1999) Radical ideas, genetic responses to oxidative stress. Clinical and Experimental Pharmacology and Physiology 26, 64–68.CrossRefGoogle ScholarPubMed
Di Ilio, C., Polidoro, G., Arduini, A., Muccini, A. and Federici, G. (1983) Glutathione peroxidase, glutathione reductase, glutathione S-transferase and gamma-glutamyl transpeptidase activities in the human early pregnancy placenta. Biochimica Medica 29, 143–148.Google Scholar
Fankboner, P.V. and Cameron, J.L. (1985) Seasonal atrophy of the visceal organs in a sea cucumber. Canadian Journal of Zoology 63, 2888–2892.CrossRefGoogle Scholar
Fuery, C.J., Withers, P.C., Hobbs, A.A. and Guppy, M. (1998) The role of protein synthesis during metabolic depression in the Australian desert frog, Neobatrachus centralis. Journal of Comparative Biochemistry and Physiology A 119, 469–476.CrossRefGoogle ScholarPubMed
Góth, L. (1991) A simple method for determination of serum catalase activity and revision of reference range. Clinica Chimica Acta 196, 143–151.CrossRefGoogle ScholarPubMed
Griffith, O.W. (1980) Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Analytical Biochemistry 106, 207–212.CrossRefGoogle ScholarPubMed
Grundy, J.E. and Storey, K.B. (1994) Urea and salt effects on enzymes from estivating and non-estivating amphibians. Molecular and Cellular Biochemistry 131, 9–17.CrossRefGoogle ScholarPubMed
Grundy, J.E. and Storey, K.B. (1998) Antioxidant defenses and lipid peroxidation damage in estivating toads, Scaphiopus couchii. Journal of Comparative Biochemistry and Physiology B 168, 132–142.Google Scholar
Heise, K., Puntarulo, S., Pörtner, H.O. and Abele, D. (2003) Production of reactive oxygen species by isolated mitochondria of the Antarctic bivalve Latemula elliptica (King and Broderip) under heat stress. Comparative Biochemistry and Physiology Part C 134, 79–90.Google ScholarPubMed
Hermes-Lima, M. and Storey, K.B. (1995) Antioxidant defenses and metabolic depression in a pulmonate land snail. American Journal of Physiology—Regulatory, Integrative and Comparative Physiology 268, 1386–1393.CrossRefGoogle Scholar
Hermes-Lima, M., Storey, J.M. and Storey, K.B. (1998) Antioxidant defenses and metabolic depression. The hypothesis of preparation for oxidative stress in land snails. Journal of Comparative Biochemistry and Physiology B 120, 437–448.Google Scholar
Hermes-Lima, M., Storey, J.M. and Storey, K.B. (2001) Antioxidant defenses and animal adaptation to oxygen availability during environmental stress. In Storey, K.B. and Storey, J.M. (eds) Cell and molecular responses to stress. Amsterdam: Elsevier Press, pp. 263–287.Google Scholar
Hermes-Lima, M. and Zenteno-Savin, T. (2002) Animal response to drastic changes in oxygen availability and physiological oxidative stress. Journal of Comparative Biochemistry and Physiology C 133, 537–556.Google Scholar
Hochachka, P.W. and Lutz, P.L. (2001) Mechanism, origin, and evolution of anoxia tolerance in animals. Journal of Comparative Biochemistry and Physiology B 130, 435–459.Google Scholar
Hudson, N.J. and Franklin, C.E. (2002) Effect of estivation on muscle characteristics and locomotor performance in the green-striped burrowing frog, Cyclorana alboguttata. Journal of Comparative Biochemistry and Physiology B 172, 177–182.Google ScholarPubMed
Jackson, D.C. (2000) Living without oxygen, lessons from the freshwater turtle. Journal of Comparative Biochemistry and Physiology A 125, 299–315.CrossRefGoogle ScholarPubMed
Ji, J.P. (1991) An ultramicroanalytic and rapid method for determination of superoxide dismutase activity. Journal of Nanjing Railway Medical College 10, 27–30.Google Scholar
Kaur, M., Atif, F., Ali, M., Rehman, H. and Raisuddin, S. (2005) Heat stress-induced alterations of antioxidants in the freshwater Channa punctata Bloch. Journal of Fish Biology 67, 1653–1665.CrossRefGoogle Scholar
Lampert, K.I. and Linsenmair, E. (2002) Alternative life cycle strategies in the West African reed frog Hyperolius nitidulus, the answer to an unpredictable environment? Oecologia 130, 364–372.Google Scholar
Land, S.C. and Bernier, N.J. (1995) Estivation, mechanisms and control of metabolic suppression. In Hochachka, P.W. and Mommolsen, T.P. (eds) Biochemistry and molecular biology of fishes. Amsterdam: Elsevier Science, pp. 381–412.Google Scholar
Li, F.X., Liu, Y.H., Song, B.X., Sun, H.L., Zhang, X.L. and Gu, B.X. (1996) Study on aestivating habit of sea cucumber Apostichopus japonicus Selenka: ecological characteristic of aestivation. Journal of Fishery Science of China 3, 49–57.Google Scholar
Li, X. and Wang, X. (2007) The histological observation of alimentary tract and respiratory tree in sea cucumber, Apostichopus japonicus during aestivation induced in lab. Journal of Dalian Fisheries University 22, 81–85.Google Scholar
Liao, Y. (1980) The Aspidochirote Holothurians of China with erection of a new genus in echinoderms, present and past. In Jangoux, M. (ed.) Proceedings of European Colloquium on Echinoderms. Rotterdam: A.A. Balkema Publishers, pp. 115–120.Google Scholar
Liu, Y.H., Li, F.X., Song, B.X., Sun, H.L., Zhang, X.L. and Gu, B.X. (1996) Study on aestivating habit of sea cucumber Apostichopus japonicus Selenka: ecological characteristic of aestivation. Journal of Fishery Science of China 3, 41–48.Google Scholar
Lutz, P.L., LaManna, J.C., Adams, M.R. and Rosenthal, M. (1980) Cerebral resistance to anoxia in the marine turtle. Respiratory Physiology and Neurobiology 41, 241–251.CrossRefGoogle ScholarPubMed
Lutz, P.L. and Nilsson, G.E. (1997) Contrasting strategies for anoxic brain survival. Glycolysis up or down? Journal of Experimental Biology 200, 411–419.CrossRefGoogle ScholarPubMed
Martinez-Alvarez, R.M., Hidalgo, M.C., Domezain, A., Morales, A.E., Garcia-Gallego, M. and Sanz, A. (2002) Physiological changes of sturgeon Acipenser naccarii caused by increasing environmental salinity. Journal of Experimental Biology 205, 3699–3706.Google Scholar
Pinder, A.W., Storey, K.B. and Ultsch, G.R. (1992) Estivation and hibernation. In Feder, M.E. and Burggren, W.W. (eds) Environmental biology of the Amphibia. Chicago: University of Chicago Press, pp. 250–274.Google Scholar
Prakash, P., Kumar, G.P., Laloraya, M., Hemnani, T. and Parihar, M.S. (1998) Superoxide anion radical generation as a temperature stress response in the gills of freshwater catfish Heteropneustes fossilis. Role in mucus exudation under elevated temperature. Journal of Comparative Biochemistry and Physiology C 119, 211–216.Google Scholar
Sloan, N.A. (1984) Echinoderm fisheries of the world: a review. In Keegan, B.F. and O'Oonnor, B.D.S. (eds) Echinodermata: Proceedings of the Fifth International Echinoderm Conference. Rotterdam: A.A. Balkema, pp. 109–124.Google Scholar
Spector, T. (1978) Refinement of the Coomassie blue method of protein quantification. Analytical Biochemistry 86, 142–146.CrossRefGoogle Scholar
Stadtman, E.R. and Levine, R.L. (2000) Protein oxidation. Annals of the New York Academy of Sciences 899, 191–208.Google Scholar
Stewart, J.M., Claude, J.F., MacDonald, J.A. and Storey, K.B. (2000) The muscle fatty acid binding protein of spadefoot toad Scaphiopus couchii. Journal of Comparative Biochemistry and Physiology B 125, 347–357.Google Scholar
Storey, K.B. (1975) Purification and properties of turtle heart creatine kinase, role of the enzyme in glycolytic control. International Journal of Biochemistry and Cell Biology 6, 53–59.Google Scholar
Storey, K.B. (2001) Turning down the fires of life, metabolic regulation of hibernation and estivation. Oxford: BIOS Scientific Publishers.Google Scholar
Storey, K.B. (2002) Life in the slow lane, molecular mechanisms of estivation. Journal of Comparative Biochemistry and Physiology A 133, 733–754.CrossRefGoogle ScholarPubMed
Storey, K.B. (2006) Anoxia tolerance in turtles, metabolic regulation and gene expression. Journal of Comparative Biochemistry and Physiology A 147, 263–276.Google Scholar
Sturla, M., Masini, M.A., Prato, P., Grattarola, C. and Uva, B. (2001) Mitochondria-rich cells in gills and skin of an African lungfish, Protopterus annectens. Cell and Tissue Research 303, 351–358.CrossRefGoogle ScholarPubMed
Wilhelm, D.F., Sell, F., Ribeiro, L., Ghislandi, M., Carrasquedo, F. and Fraga, C.G. (2002) Comparison between the antioxidant status of terrestrial and diving mammals. Comparative Biochemistry and Physiology Part A 133, 885–892.CrossRefGoogle Scholar
Winston, G.W. (1991) Oxidants and antioxidants in aquatic animals. Journal of Comparative Biochemistry and Physiology C 100, 173–176.Google Scholar
Yang, H.S., Yuan, X.T., Zhou, Y., Zhang, T., Mao, Y.Z. and Liu, Y. (2005) Effects of body size and water temperature on food consumption and growth in the sea cucumber Apostichopus japonicus Selenka with special reference to aestivation. Aquaculture Research 36, 1085–1092.Google Scholar
Yang, H.S., Zhou, Y., Zhang, T., Yuan, X.T., Li, X.X., Liu, Y. and Zhang, F.S. (2006) Metabolic characteristics of sea cucumber Apostichopus japonicus Selenka during aestivation. Journal of Experimental Marine Biology and Ecology 330, 505–510.CrossRefGoogle Scholar