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Bilateral comparison of in situ neurolipofuscin accumulation in Callinectes sapidus caught in the wild

Published online by Cambridge University Press:  10 August 2009

Cátia R. Pereira ,
Fernando D'Incao  and
Duane B. Fonseca
Cátia R. Pereira
Affiliation:
Instituto de Oceanografia, Universidade Federal do Rio Grande, Rio Grande, RS, 96201-900, Brazil
Fernando D'Incao
Affiliation:
Instituto de Oceanografia, Universidade Federal do Rio Grande, Rio Grande, RS, 96201-900, Brazil
Duane B. Fonseca*
Affiliation:
Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, 96201-900, Brazil
*
Correspondence should be addressed to: D.B. Fonseca, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, 96201-900, Brazil email: dbf1@mac.com
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Abstract

Age determination using quantification of in situ neurolipofuscin has been an useful and reliable tool to understand population dynamics of crustaceans. In the present investigation, in situ neurolipofuscin was quantified in the medulla terminalis of eyestalks (cluster A cell mass, MT-A), and in the olfactory lobe cell mass 10 (OLCM-10) of the supra-oesophageal ganglion of unknown age blue crabs (Callinectes sapidus) caught in the wild. No significant difference in neurolipofuscin quantity was found between right and left MT-A and between right and left OLCM-10. Comparison between MT-A and OLCM-10 resulted in a weaker correlation. Average neurolipofuscin was 0.353±0.038% vol. and 0.896±0.105% vol. in MT-A and OLCM-10, respectively. Size explained 23% of the variation of neurolipofuscin loading in OLCM-10. No significant relationship was found between size and MT-A neurolipofuscin content. It can be concluded that both structures are suitable for the quantification of neurolipofuscin, and they have the potential for age determination for C. sapidus.

Keywords

neurolipofuscinCrustaceablue crab
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 90 , Issue 3 , May 2010 , pp. 563 - 567
Copyright
Copyright © Marine Biological Association of the United Kingdom 2009

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References

REFERENCES

Aguilar, R., Johnson, E.G., Hines, A.H., Kramer, M.A. and Goodison, M.R. (2008) Importance of blue crab life history for stock enhancement and spatial management of the fishery in Chesapeake Bay. Reviews in Fisheries Science 16, 117–124.CrossRefGoogle Scholar
Belchier, M., Edsman, L., Sheehy, M.R.J. and Shelton, P.M.J. (1998) Estimating age and growth in long-lived temperate freshwater crayfish using lipofuscin. Freshwater Biology 39, 439–446.CrossRefGoogle Scholar
Carver, A.M., Wolcott, T.G., Wolcott, D.L. and Hines, A.H. (2005) Unnatural selection: effects of a male-focused size-selective fishery on reproductive potential of a blue crab population. Journal of Experimental Marine Biology and Ecology 319, 29–41.CrossRefGoogle Scholar
Fonseca, D.B. and Sheehy, M.R.J. (2007) Does size matter? A cautionary experiment on overoptimism in length-based bioresource management. Canadian Journal of Fisheries and Aquatic Sciences 64, 996–1008.CrossRefGoogle Scholar
Fonseca, D.B., Sheehy, M.R.J. and Shelton, P.M.J. (2003) Unilateral eyestalk ablation reduces neurolipofuscin accumulation rate in the contralateral eyestalk of a crustacean, Pacifastacus leniusculus. Journal of Experimental Marine Biology and Ecology 289, 277–286.CrossRefGoogle Scholar
Fonseca, D.B., Parise, C.K. and Barutot, R.A. (2005a) Ocurrence of lipofuscin age pigment in Chasmagnathus granulatus (Decapoda, Varunidae). Nauplius 13, 175–181.Google Scholar
Fonseca, D.B., Brancato, C.L., Prior, A.E., Shelton, P.M.J. and Sheehy, M.R.J. (2005b) Death rates reflect accumulating brain damage in arthropods. Proceedings of the Royal Society of London Series B—Biological Sciences 272, 1941–1947.Google ScholarPubMed
Fonseca, D.B., Sheehy, M.R.J., Blackman, N., Shelton, P.M.J. and Prior, A.E. (2005c) Reversal of a hallmark of brain ageing: lipofuscin accumulation. Neurobiology of Aging 26, 69–76.CrossRefGoogle ScholarPubMed
Hadi, A.S. (1994) A modification of a method for the detection of outliers in multivariate samples. Journal of the Royal Statistical Society 56, 393–396.Google Scholar
Hines, A.H. (2003) Ecology of juveniles and adult blue crabs: summary of discussion of research themes and directions. Bulletin of Marine Science 72, 423–433.Google Scholar
Maxwell, K.E., Matthews, T.R., Sheehy, M.R.J., Bertelsen, R.D. and Derby, C.D. (2007) Neurolipofuscin is a measure of age in Panulirus argus, the Caribbean spiny lobster, in Florida. Biological Bulletin. Marine Biological Laboratory, Woods Hole 213, 55–66.CrossRefGoogle ScholarPubMed
Miller, T.J. and Smith, S.G. (2003) Modeling crab growth and population dynamics: insights from the Blue Crab conference. Bulletin of Marine Science 72, 537–541.Google Scholar
Puckett, B.J., Secor, D.H. and Ju, S.J. (2008) Validation and application of lipofuscin-based age determination for Chesapeake Bay blue crabs Callinectes sapidus. Transactions of the American Fisheries Society 137, 1637–1649.CrossRefGoogle Scholar
Santos, C.R.M. (2007) Biogeografia, sistemática e filogenia de Portunidae Rafinesque, 1815 do Oceano Atlântico Ocidental (Crustacea, Decapoda): ênfase em Callinectes Stimpson, 1860 e Portunus Weber, 1795. PhD thesis. Universidade Federal do Rio Grande, Brazil, Rio Grande do Sul, Brazil.Google Scholar
Sharov, A.F., Vølstad, J.H., Davis, G.R., Davis, B.K., Lipcius, R.N. and Montane, M.M. (2003) Abundance and exploitation rate of the blue crab (Callinectes sapidus) in Chesapeake Bay. Bulletin of Marine Science 72, 543–565.Google Scholar
Sheehy, M.R.J. (2002a) A flow-cytometric method for quantification of neurolipofuscin and comparison with existing histological and biochemical approaches. Archives of Gerontology and Geriatrics 34, 233–248.CrossRefGoogle ScholarPubMed
Sheehy, M.R.J. (2002b) Role of environmental temperature in aging and longevity: insights from neurolipofuscin. Archives of Gerontology and Geriatrics 34, 287–310.CrossRefGoogle ScholarPubMed
Sheehy, M.R.J. and Prior, A.E. (2008) Progress on an old question for stock assessment of the edible crab, Cancer pagurus. Marine Ecology Progress Series 353, 191–202.CrossRefGoogle Scholar
Sheehy, M.R.J. and Shelton, P.M.J. (2001) Use of lipofuscin age pigment for age determination of lobsters. Marine Fisheries R & D Final Report, MF0215/CSA2596, 57 pp.Google Scholar
Sheehy, M.R.J., Bannister, R.C.A., Wickins, J.F. and Shelton, P.M.J. (1999) New perspectives on the growth and longevity of the European lobster (Homarus gammarus). Canadian Journal of Fisheries and Aquatic Sciences 56, 1904–1915.CrossRefGoogle Scholar
Sheehy, M.R.J., Caputi, N., Chubb, C. and Belchier, M. (1998) Use of lipofuscin for resolving cohorts of western rock lobster (Panulirus cygnus). Canadian Journal of Fisheries and Aquatic Sciences 55, 925–936.CrossRefGoogle Scholar
Sheehy, M.R.J., Greenwood, J.G. and Fielder, D.R. (1995) Lipofuscin as a record of rate of living in an aquatic poikilotherm. Journal of Gerontology 50A, 327–336.Google Scholar
Sohal, R.S. (1981) Relationship between metabolic rate, lipofuscin accumulation and lysosomal enzyme activity during aging in the adult housefly, Musca domestica. Experimental Gerontology 16, 347–355.CrossRefGoogle ScholarPubMed
Sullivan, J.M. and Beltz, B.S. (2001) Neural pathways connecting the deutocerebrum and lateral protocerebrum in the brains of decapod crustaceans. Journal of Comparative Neurology 441, 9–22.CrossRefGoogle ScholarPubMed