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The Determination of Glycollic Acid in Sea Water

Published online by Cambridge University Press:  11 May 2009

N. M. Shah  and
G. E. Fogg
N. M. Shah
Affiliation:
University Oceanographic Laboratory, Cochin 16, India
G. E. Fogg
Affiliation:
Marine Science Laboratories, Menai Bridge, Anglesey
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Extract

Glycollic acid is probably a major component of the extracellular organic fraction liberated during phytoplankton photosynthesis and presumably plays an important part in energy transfers in aquatic environments (Fogg, 1966). It has been difficult to investigate these possibilities, however, because of the lack of a reliable and convenient method for the determination of glycollate in natural waters. With fresh water, ionexchange resins have been used for separating and concentrating glycollate which may then be estimated colorimetrically using Calkins's method (Fogg, Eagle & Kinson, 1969). This method is time consuming and is much more difficult and laborious with sea water because of the presence of high concentrations of inorganic ions. Koyama & Thompson (1964) determined various organic acids after extraction from sea water with organic solvents but this method is also laborious and for glycollic acid yields ambiguous results. Andrews & Williams (1971) measured free amino acids and also glucose occurring in sea water in μtgl−1 concentrations by retaining them on a copper chelating resin and a charcoal column respectively and eluting them with ammonium hydroxide or 10% ethanol as appropriate.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 53 , Issue 2 , May 1973 , pp. 321 - 324
Copyright
Copyright © Marine Biological Association of the United Kingdom 1973

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References

Andrews, P. & Le, B. Williams P. J., 1971. Heterotrophic utilization of dissolved organic com-pounds in the sea. III. Measurement of the oxidation rates and concentrations of glucose and amino acids in sea water. Journal of the Marine Biological Association of the United Kingdom, 51, 111–125.CrossRefGoogle Scholar
Calkins, V. P., 1943. Microdetermination of glycollic and oxalic acids. Industrial and Engineering Chemistry: Analytical Edition, 15, 762–763.Google Scholar
Fogg, G. E., 1966. The extracellular products of algae. Oceanography and Marine Biology. An Annual Review, 4, 195–212.Google Scholar
Fogg, G. E., Eagle, D. J. & Kinson, Margaret E., 1969. The occurrence of glycollic acid in natural waters. Verhandlungen der Internationalen Vereinigung fur theoretische und angewandte Limnologie, 17, 480–484.Google Scholar
Kornberg, H. L. & Gotto, A. M., 1961. The metabolism of C2 compounds in microorganisms 6. Synthesis of cell constituents from glycollate by Pseudomonas sp. Biochemical Journal, 78, 69–82.CrossRefGoogle Scholar
Koyama, T. & Thompson, T. G., 1964. Identification and determination of organic acids in sea water by partition chromatography. Journal of the Oceanographical Society of Japan, 20, 209–20.CrossRefGoogle Scholar
Moed, J. R., 1971. Aluminium oxide as adsorbent for natural water-soluble yellow material. Limnology and Oceanography, 16, 140–2.CrossRefGoogle Scholar
Schindler, D. W., 1966. A liquid scintillation method for measuring carbon-14 uptake in photo-synthesis. Nature, London, 211, 844–45.CrossRefGoogle Scholar