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Development of microsatellite markers in the razor clam Solen marginatus (Bivalvia: Solenidae)

Published online by Cambridge University Press:  30 July 2007

Marta Francisco-Candeira
Affiliation:
Departamento de Biología Celular y Molecular, Universidade da Coruña, A Zapateira s/n, 15071 La Coruña, Spain
Ana González-Tizón
Affiliation:
Departamento de Biología Celular y Molecular, Universidade da Coruña, A Zapateira s/n, 15071 La Coruña, Spain Instituto Universitario de Estudos Marítimos, Universidade da Coruña, Campus de Elviña, Ed. Servizos Centrais de Investigación, 15071 La Coruña, Spain
Miguel A. Varela
Affiliation:
Departamento de Biología Celular y Molecular, Universidade da Coruña, A Zapateira s/n, 15071 La Coruña, Spain Instituto Universitario de Estudos Marítimos, Universidade da Coruña, Campus de Elviña, Ed. Servizos Centrais de Investigación, 15071 La Coruña, Spain
Andrés Martínez-Lage
Affiliation:
Departamento de Biología Celular y Molecular, Universidade da Coruña, A Zapateira s/n, 15071 La Coruña, Spain Instituto Universitario de Estudos Marítimos, Universidade da Coruña, Campus de Elviña, Ed. Servizos Centrais de Investigación, 15071 La Coruña, Spain

Abstract

Four microsatellite loci in the razor clam Solen marginatus are described. Loci were isolated from the sequences of intersimple sequence repeat (ISSR) markers and an enriched library. Detailed analysis of these sequences led to the design of eight primer pairs. Allelic variation was assessed in 20 individuals from Redondela, Spain. The genetic variation observed in the markers presented here will be useful for future studies on the population structure of Solen marginatus in the wild and for aquaculture of this species.

Type
Brief Report
Copyright
2007 Marine Biological Association of the United Kingdom

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