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Effects of sulphide on anoxia-driven mortality and anaerobic metabolism in the ark shell Anadara kagoshimensis

Published online by Cambridge University Press:  21 March 2016

Yasushi Miyamoto Open the ORCID record for Yasushi Miyamoto [Opens in a new window]  and
Chitose Iwanaga
Yasushi Miyamoto*
Affiliation:
Environmental Sanitation Research Center, Tottori Prefecture, 526-1, Minamidani, Yurihama-cho, Tottori 682-0704, Japan
Chitose Iwanaga
Affiliation:
Environmental Sanitation Research Center, Tottori Prefecture, 526-1, Minamidani, Yurihama-cho, Tottori 682-0704, Japan
*
Correspondence should be addressed to: Y. Miyamoto, Environmental Sanitation Research Center, Tottori Prefecture, 526-1, Minamidani, Yurihama-cho, Tottori, 682-0704, Japan Email: scapharca@gmail.com
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Abstract

Reduction in survival under hypoxic conditions in the presence of sulphide has been repeatedly demonstrated in various benthic invertebrates. However, the reason for this reduction has not yet been clearly elucidated. In this study, the effects of sulphide accumulation on anoxic survival and anaerobic metabolism were investigated in the ark shell Anadara kagoshimensis. Ark shells from western Japan were experimentally exposed to 3 sulphide-accumulation levels under sustained anoxic conditions: accumulated H2S treatment (static incubation), decreased H2S treatment (semi-static incubation with daily replacement of incubation media), and inhibited H2S treatment (static incubation with the addition of antibiotics). Moreover, the effect of antibiotics on anoxic survival was examined under sulphide exposure. The decreased H2S and inhibited H2S treatments resulted in 1.5- and 3-fold increase in the anoxic survival time, respectively, when compared with the accumulated H2S treatment. Under anoxic sulphide exposure, the antibiotics addition did not affect survival time, suggesting the shorter survival time in the accumulated H2S incubation was probably due to sulphide toxicity. Glycogen consumption and propionate accumulation, which indicate activation of anaerobic metabolism, were observed in both accumulated and inhibited H2S treatments. However, glycogen consumption was significantly higher in the accumulated H2S treatment after a significant sulphide accumulation was detected in the incubation media. In addition, survival in the accumulated H2S treatment decreased rapidly, whereas no significant mortality was observed in the inhibited H2S throughout the experiment. These results likely suggest that the accelerated anoxic-driven mortality in sulphide-rich environments was partly due to the faster breakdown of glycogen.

Keywords

Anadara kagoshimensisanaerobic metabolismglycogenhypoxiamortalitysulphide
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 97 , Issue 2 , March 2017 , pp. 329 - 336
Copyright
Copyright © Marine Biological Association of the United Kingdom 2016 

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