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Long-term storage of microalgae: determination of optimum cryopreservation conditions

Published online by Cambridge University Press:  01 August 2022

Irem Deniz Open the ORCID record for Irem Deniz [Opens in a new window] ,
Zeliha Demirel Open the ORCID record for Zeliha Demirel [Opens in a new window] ,
Esra Imamoglu  and
Meltem Conk-Dalay Open the ORCID record for Meltem Conk-Dalay [Opens in a new window]
Irem Deniz*
Affiliation:
Bioengineering Department, Faculty of Engineering, Manisa Celal Bayar University, 45119, Yunusemre/Manisa, Turkey
Zeliha Demirel
Affiliation:
Bioengineering Department, Faculty of Engineering, Ege University, 35100, Bornova/Izmir, Turkey
Esra Imamoglu
Affiliation:
Bioengineering Department, Faculty of Engineering, Ege University, 35100, Bornova/Izmir, Turkey
Meltem Conk-Dalay
Affiliation:
Bioengineering Department, Faculty of Engineering, Ege University, 35100, Bornova/Izmir, Turkey
*
Author for correspondence: Irem Deniz, E-mail: iremdenz@gmail.com
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Abstract

Maintenance of eukaryotic microalgae strains for the long term is generally carried out using serial subculture techniques which require labour, time and cost. Cryopreservation techniques provide long-term storage of up to years for numerous microorganism strains and cell cultures. Ssu930ijn vbvbhnn8;l,n is related to a successfully designed mass and heat transfer balance throughout the cell. In this study, optimization of the cryopreservation process was carried out for two commercially used microalgal strains. The parameters to be optimized were DMSO percentage (0–25%), incubation time (1–15 min) and cryopreservation term (7–180 days) using a central composite design (CCD). Long-term storage up to 123.17 and 111.44 days corresponding to high cell viabilities was achieved for Chlorella vulgaris and Neochloris texensis, respectively. Generated models were found to be in good agreement with experimental results. The study also revealed holistic results for storage of microalgal strains in a stable state for industrial applications.

Keywords

Chlorella vulgariscryopreservationmicroalgaeNeochloris texensisoptimization
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 102 , Issue 3-4 , June 2022 , pp. 276 - 284
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of Marine Biological Association of the United Kingdom

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