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Microscopy of the absorptive cells and gut-associated lymphoid tissue of the flounder Platichthys flesus

Published online by Cambridge University Press:  11 May 2009

P. G. Jenkins ,
A. L. Pulsford  and
J. E. Harris
P. G. Jenkins
Affiliation:
Plymouth Marine Laboratory, Citadel Hill, Plymouth, PL1 2PB Department of Biological Sciences, Polytechnic Southwest, Drake's Circus, Plymouth
A. L. Pulsford
Affiliation:
Plymouth Marine Laboratory, Citadel Hill, Plymouth, PL1 2PB
J. E. Harris
Affiliation:
Department of Biological Sciences, Polytechnic Southwest, Drake's Circus, Plymouth
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Extract

A light and electron microscopic study of the enterocytes and gut-associated lymphoid tissue (G ALT) of the flounder Platichthys flesus showed regional differences in the structure of the epithelial cells. Epithelial villi decreased in height and spatial complexity, but increased in overall thickness in the posterior intestine. There were no specialized cells comparable with mammalian M cells. Periodic acid Schiff (PAS) activity increased in the enterocytes and goblet cells in the anterior intestine where alkaline phosphatase activity was most pronounced in enterocytes. Adjacent cells were joined by tight junctions.

Gut-associated lymphoid tissue was composed of isolated leucocytes in the epithelium and lamina propria with no accumulations. Numbers of intra-epithelial leucocytes were not statistically different in the various regions of the intestine. Five leucocyte types were distinguished among the gut-associated lymphoid tissue although lymphocytes and granulocytes were the most common. Granulocytes were positive for the Romanowsky stains with PAS/Alcian blue positive granules. Blast cells similar to large lymphocytes were unevenly distributed through the different regions of the intestine. Plasma cells were more numerous in the epithelium than in the lamina propria. Macrophages were rarely detected.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 72 , Issue 3 , August 1992 , pp. 553 - 567
Copyright
Copyright © Marine Biological Association of the United Kingdom 1992

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References

Barber, D.L. & Mills-Westermann, J.E., 1978. Occurrence of the periodic acid-Schiff positive granular leucocyte (PAS-GL) in some fishes and its significance. Journal of Fish Biology, 12, 35–43.CrossRefGoogle Scholar
Bergeron, T. & Woodward, B., 1983. Ultrastructure of the granule cells in the small intestine of the rainbow trout (Salmo gairdneri) before and after stratum granulosum formation. Canadian journal of Zoology, 61, 133–138.CrossRefGoogle Scholar
Bishop, C. & Odense, P.H., 1966. Morphology of the digestive tract of the cod, Gadus morhua. Journal of the Fisheries Research Board of Canada, 23, 1607–1615.CrossRefGoogle Scholar
Bucke, D., 1971. The anatomy and histology of the alimentary tract of the carnivorous fish the pike Esox lucius L. Journal of Fish Biology, 3, 421–431.CrossRefGoogle Scholar
Buddington, R.K. & Doroshev, S.I., 1986. Structural and functional relations of the white sturgeon alimentary canal (Acipenser transmontanus). Journal of Morphology, 190, 201–213.CrossRefGoogle ScholarPubMed
Burnstock, G., 1959. The morphology of the gut of the brown trout (Salmo trutta). Quarterly Journal of Microscopical Sciences, 100, 183–198.Google Scholar
Caceci, T., 1984. Scanning electron microscopy of goldfish, Carassius auratus, intestinal mucosa. Journal of Fish Biology, 25, 1–12.CrossRefGoogle Scholar
Davina, J.H.M., Rikjers, G.T., Rombout, J.H.W.M., Timmermans, L.P.M. & Van Muiswinkel, W.B., 1980. Lymphoid and non-lymphoid cells in the intestine of cyprinid fish. In Development and differentiation of vertebrate lymphocytes (ed. J.B., Horton), pp. 129–140. Amsterdam: Academic Press.Google Scholar
Dawes, B., 1929. The histology of the alimentary tract of the plaice (Pleuronectes platessa). Quarterly Journal of Science, 73, 243–274.Google Scholar
Doggett, T.A., 1989. The structure and function ofperipheral blood leucocytes and gut-associated lymphoid tissue in the cichlid Oreochromis mossambicus. PhD thesis, Plymouth Polytechnic.Google Scholar
Doggett, T.A., 1990, in press. Gut-associated lymphoid tissue (GALT) in Oreochromis mossambicus.Google Scholar
Di Conza, J.J. & Halliday, W.J., 1971. Relationship of catfish serum antibodies to immunoglobulin in mucus secretions. Australian Journal of Experimental Biology and Medical Science, 49, 517–519.CrossRefGoogle ScholarPubMed
Drury, R.A.B. & Wallington, E.A., 1980. Carletons histological technique. 5th ed. Oxford: Oxford University Press.Google Scholar
Elbal, M.T. & Agulleiro, B., 1986. A histochemical and ultrastructural study of the gut of Sparus auratus (Teleostei). Journal of Submicroscopical Cytology, 18, 335–347.Google Scholar
Ezeasor, D.N. & Stokoe, W.M., 1980. Scanning electron microscopic study of the gut mucosa of the rainbow trout Salmo gairdneri Richardson. Journal of Fish Biology, 17, 529–539.CrossRefGoogle Scholar
Ezeasor, D.N. & Stokoe, W.M., 1981. Light and electron microscopic studies on the absorptive cells of the intestine, caeca and rectum of the adult rainbow trout, Salmo gairdneri, Rich. Journal of Fish Biology, 18, 527–544.CrossRefGoogle Scholar
Fange, R., 1986. Lymphoid organs in sturgeons (Acipensiridae). In Fish immunology (ed. J.S., Stolenet al.), pp. 153–161. Amsterdam: Elsevier.Google Scholar
Ferguson, A., 1977. Intraepithelial enterocytes of the small intestine. Gut, 18, 921–937.CrossRefGoogle Scholar
Fichtelius, K.E., Finstad, J. & Good, R. A., 1966. The phylogenetic occurrence of lymphocytes within the gut epithelium. International Archives of Allergy and Applied Immunology, 35, 119–133.CrossRefGoogle Scholar
Gas, N. & Noailliac-Depeyre, J., 1976. Studies on intestinal epithelium involution during prolonged fasting, journal of infrastructure Research, 56, 137–151.Google ScholarPubMed
Georgopoulou, U., Dabrowski, K., Sire, M.F. & Vernier, J.M., 1988. Absorption of intact proteins by the intestinal epithelium of trout, Salmo gairdneri. A luminescence enzyme immunoassay and cytochemical study. Cell and Tissue Research, 251, 145–152.CrossRefGoogle ScholarPubMed
Giraud, A.S., Yeomans, N.D. & St John, D.J.B., 1979. Ultrastructure and cytochemistry of the gastric mucosa of a reptile, Tiliqua scincoides. Cell and Tissue Research, 197, 281–294.CrossRefGoogle ScholarPubMed
Hart, S., Wrathmell, A.B. & Harris, J.E., 1986. Gut associated lymphoid tissue in the dogfish Scyliorhinus canicula: a light microscopic study. Journal of the Marine Biological Association of the United Kingdom, 66, 721–729.CrossRefGoogle Scholar
Hart, S., Wrathmell, A.B., Harris, J.E. & Doggett, T.A., 1987. Gut-associated lymphoid tissue (GALT) in the common dogfish Scyliorhinus canicula L.: an ultrastructural study. Journal of the Marine Biological Association of the United Kingdom, 67, 639–645.CrossRefGoogle Scholar
Hart, S., Wrathmell, A.B., Harris, J.E. & Grayson, T.H., 1988. Gut immunology in a fish: a review. Developmental and Comparative Immunology, 12, 453–480.CrossRefGoogle Scholar
Hayhoe, F.G.J. & Flemens, J., 1969. An atlas of haematological cytology. 2nd ed. London: Wolf Publications.Google Scholar
Junger, H., Kotrschal, K. & Goldschmid, A., 1989. Comparative morphology and ecomorphology of the gut in European cyprinids (Teleostei). Journal of Fish Biology, 34, 315–326.CrossRefGoogle Scholar
Kimura, N., 1973. Fine structure of the epithelial cells in the pyloric caecum of the rainbow trout, Salmo gairdneri. Japanese Journal of Ichthyology, 20, 13–24.Google Scholar
Krementz, A.B. & Chapman, G.B., 1975. Ultrastructure of the posterior half of the intestine of the channel catfish, Ictalurus punctatus. Journal of Morphology, 145, 441–449.CrossRefGoogle ScholarPubMed
Macdonald, N.L., 1987. An electron microscopic examination of the gastrointestinal epithelium in Dover sole, Solea solea (L). Journal of Fish Biology, 31, 27–36.CrossRefGoogle Scholar
Marsh, M.N. & Swift, J.A., 1969. A study of the small intestine mucosa using the scanning electron microscope. Gut, 10, 940–949.CrossRefGoogle ScholarPubMed
McClean, E. & Ash, R., 1986. The time-course of appearance and net accumulation of horseradish peroxidase (HRP) presented orally to juvenile carp Cyprinus carpio (L.). Comparative Biochemistry and Physiology, 84A, 687–690.CrossRefGoogle Scholar
McClean, E. & Ash, R., 1987. The time-course of appearance and net accumulation of horseradish peroxidase (HRP) presented orally to rainbow trout Salmo gairdneri (Richardson). Comparative Biochemistry and Physiology, 88A, 507–510.CrossRefGoogle Scholar
McLean, E. & Ash, R., 1990. Modified uptake of the protein antigen horseradish peroxidase (HRP), following oral delivery to rainbow trout, Oncorhynchus mykiss. Aquaculture, 87, 373–379.CrossRefGoogle Scholar
Mowat, A.M., 1987. The cellular basis of gastrointestinal immunity. In Immunopathology of the small intestine (ed. M.N., Marsh), pp. 41–71. Florida: CRC Press.Google Scholar
Page, M. & Rowley, A.F., 1982. Morphological studies of lymphoid tissues in larval lampreys. Developmental and Comparative Immunology, supplement 2, 35–40.Google Scholar
Pearse, A.G.E., 1968. Histochemistry, theoretical and applied. 3rd ed., vol. 1. London: Churchill Press.Google Scholar
Pontius, H. & Ambrosius, H., 1972. Beitrace zur immunobiologie poikilothermertionen der knockenfische am biespel des flussbarsches (Percafluviatilis). Acta Biologica et Medica Germanica, 29, 319–339.Google Scholar
Rombout, J.H.W.M. & Van Den Berg, A.A., 1989. Immunological importance of the second gut segment of carp. I. Uptake and processing of antigens by epithelial cells and macrophages. Journal of Fish Biology, 35, 13–23.CrossRefGoogle Scholar
Siankowa, L., 1966. The surface area of the intestinal mucosa in bream Abramis brama (L). Studia Societatis Scientiarum Torunensis, 8(2), 1–53.Google Scholar
Sis, R.F., Ives, P.J., Jones, D.M., Lewis, D.H. & Haensly, W.E., 1979. The microscopic anatomy of the oesophagus, stomach and intestine of the channel catfish, Ictalurus punctatus. Journal of Fish Biology, 14, 179–186.CrossRefGoogle Scholar
Stroband, H.W.J., Meer, H.V.D. & Timmermanns, L.P.M., 1979. Regional functional differentiation in the gut of the grasscarp, Ctenopharyngodon odella (Val). Histochemistry, 64, 235–249.CrossRefGoogle Scholar
Temkin, R.J. & McMillan, D.B., 1986. Gut-associated lymphoid tissue (GALT) of the goldfish, Carassius auratus. Journal of Morphology, 190, 9–26.CrossRefGoogle ScholarPubMed
Tomonaga, S., Hirokane, T. & Awaya, K., 1973. Lymphoid cells in hagfish. Zoological Magazine of the Zoological Society of Japan, 82, 133–135.Google Scholar
Tomonaga, S., Kobayashi, K., Hagiwara, K., Yamaguchi, K. & Awaya, K., 1986. Gut-associated lymphoid tissue in elasmobranchs. Zoological Science, 3, 453–458.Google Scholar
Weinberg, S., 1975. Occurrence of lymphoid cells in the intestine of the goldfish. Bijdragen Tot de Dierkunde, 45, 195–204.CrossRefGoogle Scholar
Weisel, G.F., 1973. Anatomy and histology of the digestive system of the paddlefish (Polydon spathula). Journal of Morphology, 140, 243–251.CrossRefGoogle Scholar
Williams, J.A. & Nickol, B.B., 1989. Histological structure of the intestine and pyloric caeca of the green sunfish, Lepomis cyanellus Rafinesque. Journal of Fish Biology, 35, 359–372.CrossRefGoogle Scholar
Yamamoto, T., 1966. An electron microscope study of the columnar epithelial cell in the intestine of freshwater teleosts: goldfish (Carassius auratus) and rainbow trout (Salmo irideus). Zeitschrift für Zellforschung und Mikroskopische Anatomie, 72, 66–87.CrossRefGoogle ScholarPubMed
Zapata, A., 1979. Ultrastructure of the gut-associated lymphoid tissue (GALT) of Rutilus mtilis. Morfologia Normala si Patologica, section A, 3, 23–39.Google Scholar