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New evidence of serotonin involvement in the neurohumoral control of crinoid arm regeneration: effects of parachlorophenylanine and methiothepin

Published online by Cambridge University Press:  03 August 2009

Michela Sugni ,
Iain C. Wilkie ,
Paolo Burighel  and
M. Daniela Candia Carnevali
Michela Sugni*
Affiliation:
Dipartimento di Biologia, Università degli Studi di Milano, Milan, Italy, I-20133
Iain C. Wilkie
Affiliation:
Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow, Scotland, G4 0BA
Paolo Burighel
Affiliation:
Dipartimento di Biologia, Università di Padova, Padova, Italy, I-35121
M. Daniela Candia Carnevali
Affiliation:
Dipartimento di Biologia, Università degli Studi di Milano, Milan, Italy, I-20133
*
Correspondence should be addressed to: M. Sugni, Dipartimento di Biologia, Università degli Studi di Milano, Milan, Italy, I-20133 email: michela.sugni@unimi.it
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Abstract

As well as acting as a neurotransmitter, serotonin is also involved in morphogenetic signalling during crucial phases of many developmental and regenerative processes such as cleavage, migration and differentiation. Echinoderms display nerve-dependent regenerative phenomena and serotonin is one of the main neural regulatory factors that have been identified in them. The present work was designed to investigate the broad-spectrum involvement of this molecule in echinoderm regeneration, focusing on arm regeneration in the crinoid Antedon mediterranea. We carried out specific in vivo exposure experiments to selected anti-serotonergic drugs (a synthesis inhibitor: parachlorophenylalanine and a receptor antagonist: methiothepin) and explored their possible effects on arm regeneration. Drug exposure appeared to affect regeneration, causing a general delay of regenerative growth and several histological anomalies mainly in the muscles of the stump. In addition, exposure to the antagonist produced a marked reduction of cell proliferation in both the regenerate and the stump. Our results provide further evidence that serotonin has wider functions than those related to interneuronal communication and that it may be a critical signalling molecule in the main processes of regeneration such as proliferation, morphogenesis and differentiation.

Keywords

invertebratesneuroendocrine signalregenerative developmentdifferentiationproliferation
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 90 , Issue 3 , May 2010 , pp. 555 - 562
Copyright
Copyright © Marine Biological Association of the United Kingdom 2009

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