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Temporal genetic variation of microsatellite markers in the razor clam Ensis arcuatus (Bivalvia: Pharidae)

Published online by Cambridge University Press:  23 June 2009

Miguel A. Varela ,
Andrés Martínez-Lage  and
Ana M. González-Tizón
Miguel A. Varela
Affiliation:
Departamento de Biología Celular y Molecular, Universidade da Coruña, Campus de A Zapateira s/n, E-15071 La Coruña, Spain
Andrés Martínez-Lage
Affiliation:
Departamento de Biología Celular y Molecular, Universidade da Coruña, Campus de A Zapateira s/n, E-15071 La Coruña, Spain
Ana M. González-Tizón*
Affiliation:
Departamento de Biología Celular y Molecular, Universidade da Coruña, Campus de A Zapateira s/n, E-15071 La Coruña, Spain
*
Correspondence should be addressed to: A.M. González-Tizón, Departamento de Biología Celular y Molecular, Universidade da Coruña, Campus de A Zapateira s/n, E-15071 La Coruña, Spain email: hakuna@udc.es
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Abstract

The aim of this study was to characterize new microsatellite loci in the razor clam Ensis arcuatus (Bivalvia: Pharidae) and examine the temporal genetic variability of a natural bed in Cies Islands (Galicia, north-western Spain) exploited by apnoea divers and affected by the ‘Prestige’ oil tanker spill in November 2002. In this work, we characterized four polymorphic microsatellites using an alternative approach that relies on the amplification and sequencing of ISSR markers. Observed heterozygosities ranged from 0.1053–0.6800 and number of alleles from 4–19. Linkage equilibrium was observed in all loci and two of them showed significant deviations from Hardy–Weinberg equilibrium. Estimators of FST between samples were low (<0.05) and not different from zero with a confidence level of 5%. We did not detect a clear decreasing tendency in genetic diversity although we found a significant change in allelic frequencies among samples (P = 0.0024) after the ‘Prestige’ oil spill. We propose that both phenomena could be related to a high variance in genetic success and/or a movement of adults or larvae from different source populations.

Keywords

Ensis arcuatusgenetic diversitymicrosatellites‘Prestige’ oil spillrazor clam
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 89 , Issue 8 , December 2009 , pp. 1703 - 1707
Copyright
Copyright © Marine Biological Association of the United Kingdom 2009

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