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An ensemble of new techniques to study soft-X-ray-induced variations in cellular metabolism

Published online by Cambridge University Press:  01 July 2004

EDMOND TURCU ,
RICK ALLOT ,
NICOLA LISI ,
DIMITRI BATANI ,
FULVIA BORTOLOTTO ,
ALESSANDRA MASINI ,
MARZIALE MILANI ,
MONICA BALLERINI ,
LORENZO FERRARO  and
ACHILLE POZZI
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EDMOND TURCU
Affiliation:
Rutherford Appleton Laboratory, Chilton Didcot, UK
RICK ALLOT
Affiliation:
Rutherford Appleton Laboratory, Chilton Didcot, UK
NICOLA LISI
Affiliation:
Rutherford Appleton Laboratory, Chilton Didcot, UK
DIMITRI BATANI
Affiliation:
Dipartimento di Fisica, “G.Occhialini,” Università degli Studi di Milano–Bicocca, and INFM, Milano, Italy
FULVIA BORTOLOTTO
Affiliation:
Dipartimento di Fisica, “G.Occhialini,” Università degli Studi di Milano–Bicocca, and INFM, Milano, Italy
ALESSANDRA MASINI
Affiliation:
Dipartimento di Fisica, “G.Occhialini,” Università degli Studi di Milano–Bicocca, and INFM, Milano, Italy
MARZIALE MILANI
Affiliation:
Dipartimento di Scienza dei Materiali, Università degli Studi di Milano–Bicocca, and INFM, Milano, Italy
MONICA BALLERINI
Affiliation:
Dipartimento di Scienza dei Materiali, Università degli Studi di Milano–Bicocca, and INFM, Milano, Italy
LORENZO FERRARO
Affiliation:
Dipartimento di Scienza dei Materiali, Università degli Studi di Milano–Bicocca, and INFM, Milano, Italy
ACHILLE POZZI
Affiliation:
Dipartimento di Scienza dei Materiali, Università degli Studi di Milano–Bicocca, and INFM, Milano, Italy
FABIO PREVIDI
Affiliation:
Dipartimento di Elettronica e Informazione, Politecnico di Milano, Milano, Italy
LORENZO REBONATO
Affiliation:
Dipartimento di Elettronica e Informazione, Politecnico di Milano, Milano, Italy
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Abstract

An ensemble of new techniques has been developed to study cell metabolism. These include: CO2 production monitoring, cell irradiation with soft X rays produced with a laser-plasma source, and study of oscillations in cell metabolic activity via spectral analysis of experimental records. Soft X-rays at about 0.9 keV, with a very low penetration in biological material, were chosen to produce damages at the metabolic level, without great interference with DNA activity. The use of a laser-plasma source allowed a fast deposition of high doses. Monitoring of CO2 production allowed us to measure cell metabolic response immediately after irradiation in a continuous and noninvasive way. Also a simple model was developed to calculate X-ray doses delivered to the different cell compartments following a Lambert–Bouguet–Beer law. Results obtained on Saccharomyces cerevisiae yeast cells in experiments performed at Rutherford Appleton Laboratory are presented.

Keywords

Laser plasmasMetabolismRadiobiologySoft X-raysX-ray dosimetryX-ray spectroscopyYeast
Type
Research Article
Information
Laser and Particle Beams , Volume 22 , Issue 3 , July 2004 , pp. 323 - 333
Copyright
© 2004 Cambridge University Press

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