Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-13T06:37:09.307Z Has data issue: false hasContentIssue false
  • English
  • Français

X-ray microscopy of living multicellular organisms with the Prague Asterix Iodine Laser System

Published online by Cambridge University Press:  25 March 2004

T. DESAI ,
D. BATANI ,
A. BERNARDINELLO ,
G. POLETTI ,
F. ORSINI ,
J. ULLSCHMIED ,
L. JUHA ,
J. SKALA ,
B. KRALIKOVA  and
E. KROUSKY
...Show all authors
T. DESAI
Affiliation:
Dipartimento di Fisica “G.Occhialini” and INFM, Università, di Milano Bicocca, Milano, Italy
D. BATANI
Affiliation:
Dipartimento di Fisica “G.Occhialini” and INFM, Università, di Milano Bicocca, Milano, Italy
A. BERNARDINELLO
Affiliation:
Dipartimento di Fisica “G.Occhialini” and INFM, Università, di Milano Bicocca, Milano, Italy
G. POLETTI
Affiliation:
Istituto di Fisiologia Generale e Chimica Biologica, Università di Milano, Milano, Italy
F. ORSINI
Affiliation:
Istituto di Fisiologia Generale e Chimica Biologica, Università di Milano, Milano, Italy
J. ULLSCHMIED
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
L. JUHA
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
J. SKALA
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
B. KRALIKOVA
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
E. KROUSKY
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
M. PFEIFER
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
C. KADLEC
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
T. MOCEK
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
A. PRÄG
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
O. RENNER
Affiliation:
Prague Asterix Iodine Laser System Research Centre, Prague, Czech Republic
F. COTELLI
Affiliation:
Dipartimento di Biologia, Università di Milano, Milano, Italy
C. LORA LAMIA
Affiliation:
Dipartimento di Biologia, Università di Milano, Milano, Italy
A. ZULLINI
Affiliation:
Dipartimento di Biotecnologie e Bioscienze, Università di Milano Bicocca, Milano, Italy
Get access Rights & Permissions [Opens in a new window]

Abstract

Soft X-ray contact microscopy (SXCM) experiments have been performed using the Prague Asterix Iodine Laser System (PALS). Laser wavelength and pulse duration were λ = 1.314 μm and τ (FWHM) = 450 ps, respectively. Pulsed X rays were generated using teflon, gold, and molybdenum targets with laser intensities I ≥ 1014 W/cm2. Experiments have been performed on the nematodes Caenorhabditis elegans. Images were recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Our preliminary results indicate the suitability of the SXCM for multicellular specimens.

Keywords

Atomic force microscopyLaser-produced plasmasMulticellular Caenorhabditis elegansSoft X-ray contact microscopy
Type
Research Article
Information
Laser and Particle Beams , Volume 21 , Issue 4 , October 2003 , pp. 511 - 516
Copyright
© 2003 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bargmann, C.I. & Avery, L. (1995). Laser Killing of cells in Caenorhabditis elegans. Methods Cell Biol. 48, 225–249.Google Scholar
Batani, D., Masini, A., Lora Lamia Donin, C., Cotelli, F., Previdi, F., Milani, M., Faral, B., Conte, E., Moret M., Poletti, G., &Pozzi, A. (1998). Characterisation of Saccharomyces cerevisiae yeast cells. Phys. Medica 15, 151–157.Google Scholar
Batani, D., Botto, C., Bortoloto, F., Masini, A., Bernardinello, A., Moret, M., Milani, M., Eidmann, K., Poletti, G., Cotelli, F., Donin, C.L.L., Piccoli, S., Stead, A., Ford, T., Marranca, A., Flora, F., Palladino, L. & Reale, L. (2000). Contact X-ray microscopy using the Astrix laser source. Phys. Medica 16, 49–55.Google Scholar
Bortolotto, F., Batani, D., Masini, A., Previdi, F., Rebonato, L. & Tureu, E. (2000). Study of a X-ray laser-plasma source for radiobiological experiments, microdosimetry analysis and plasma characterisation. Euro. Phys. J. D 11, 309.Google Scholar
Cox, G.N., Staprans, S. & Edgar, R.S. (1981). The cuticle of Caenorhabditis elegans. Dev. Biol. 86, 456–470.Google Scholar
Fletcher, J., Cotton, R.A. & Webb, C. (1992). Soft x-ray contact microscopy using laser generated plasma sources. Proc. SPIE 1741, 142–153.Google Scholar
Ford, T.W., Stead, A.D. & Cotton R.A. (1991). Soft x-ray contact microscopy of biological materials. Electron Microsc. Rev. 4, 269–292.Google Scholar
Ford, T.W., Page, A.M., Foster, G.F. & Stead, A.D. (1992). Effects of soft x-ray irradiation on cell ultrastructure. Proc. SPIE 1741, 325–332.Google Scholar
Foster, G.F., Buckley, C.J., Bennett, P.M. & Burge, R.E. (1992). Investigation of radiation damage to biological specimens at water window wavelengths. Rev. Scient. Instr. 63, 599–600.Google Scholar
Henke, B.L. & Jaanimagi, P.A. (1985). Two-channel, elliptical analyzer spectrograph for absolute, time-resolving time-integrating spectrometry of pulsed x-ray sources in the 100–10000-eV region. Rev. Sci. Instrum. 56, 1537–1552.Google Scholar
Henke, B.L. (1986). Low-energy x-ray response of photographic films. Mathematical models. J. Opt. Soc. Am. B 3, 11, 1540–1546.Google Scholar
Jungwirth, K., Cejnarova, A., Juha, L., Kralikova, B., Krasa, J., Drousky, E., Krupickova, P., Laska, L., Masek, K., Mocek, T., Pfeifer, M., Präg, A., Renner, O., Rohlena, K., Rus, B., Skala, J., Straka, P. & Ullschmied, J. (2001). The Prague Astrix Laser System. Phys. Plasmas 8, 2495–2501.Google Scholar
Kondo, K. & Tomie, T. (1994). Optimization of a laser-plasma X-ray source for contact X-ray microscopy. J. Appl. Phys. 75, 3798–3805.Google Scholar
Lee, R.W. (1990). User Manual for RATION. Livermore, CA: Lawrence Livermore National Laboratory.
Masini, A., Batani, D., Previdi, F., Conti, A., Pisani, F., Botto, C., Bortolotto, F., Torsiello, F., Tureu, E., Allot, R., Lisi, N., Milani, M., Costato, M., Pozzi, A. & Koenig, M. (1997). X-ray irradiation of yeast cells. Proc. SPIE 3157, 203–217.Google Scholar
Nobel Foundation. (2002). The Nobel Prize in Physiology or Medicine. Press Release, October 7.
Stead, A.D., Cotton, R.A., Page, A.M., Dooley, M. & Ford, T.W. (1992). Visualization of the effects of electron microscopy fixatives on the structure of hydrated epidermal hairs of tomato (Lycopersicum peruvianum) as revealed by soft X-ray contact microscopy. Proc. SPIE 1741, 351–362.Google Scholar
Stead, A.D., Page, A.M., Cotton, R.A., Neely, D., Bagby, R., Miura, E., Tomie, T., Shimizue, S., Majima, T., Anastasi, P.A.F. & Ford, T.W. (1995b). Modifications for soft X-ray contact microscopy-quantification of carbon density discrimination and stereo imaging. Proc. SPIE 2523, 202–211.Google Scholar
Stead, A.D., Page, A.M. & Ford, T.W. (1995a). The prospects for soft X-ray contact microscopy using laser plasmas as a X-ray source. Proc. SPIE 2523, 40–50.Google Scholar
Rockett, P.D., Bird, C.R., Hailey, C.J., Sullivan, D., Brown, D.B. & Burkhalter, P.G. (1985). X-ray calibration of Kodak Direct Exposure film. Appl. Opt. 24, 2536–2542.Google Scholar
Tallents, G.J. (1987). X-ray spectroscopy of tokamaks. JET Report No. JET-P(87) 56.