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Amplification of Fungal rDNA-Its Regions from Non-Fertile Specimens of the Lichen-Forming Genus Parmelia

Published online by Cambridge University Press:  28 March 2007

Ana Crespo ,
Paul D. Bridge  and
David L. Hawksworth
Ana Crespo
Affiliation:
Departamento de Biologia Vegetal II, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Paul D. Bridge
Affiliation:
International Mycological Institute, Bakeham Lane, Egham, Surrey TW20 9TY, UK.
David L. Hawksworth
Affiliation:
International Mycological Institute, Bakeham Lane, Egham, Surrey TW20 9TY, UK.
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Abstract

A method is described for the selective amplification of single mycobiont rDNA sequences from vegetative, non-ascomatal material of the lichenized thalli of Parmelia sulcata and P. saxatilis. The method includes a simple DNA extraction procedure employing rhizines, which avoids the use of liquid nitrogen disruption methods and phenol based extractions. The resulting amplified DNA is suitable for restriction enzyme analysis, and can be used to provide material for investigating population distribution and species concepts.

Type
Research Article
Information
The Lichenologist , Volume 29 , Issue 3 , May 1997 , pp. 275 - 282
Copyright
Copyright © British Lichen Society 1997

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