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Low-light recovery effects on assessment of photoinhibition with chlorophyll fluorescence in lichens

Published online by Cambridge University Press:  26 January 2018

Knut Asbjørn SOLHAUG
Knut Asbjørn SOLHAUG*
Affiliation:
Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, P. O. Box 5003, 1432 Ås, Norway. Email: knut.solhaug@nmbu.no
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Abstract

Chlorophyll a fluorescence is often used to estimate various types of damage in lichens. In order to optimize the output and improve interpretations of such measurements the protocol for pretreatment and measuring is important. To study the effects of measurement conditions, the lichens Lobaria pulmonaria, L. scrobiculata, Xanthoria parietina and Parmelia sulcata were first stressed by high light intensities at 600 or 1000 µmol photons m−2 s−1 for 4 h. Then various conditions during recovery or pretreatment were used to optimize the detection of more lasting damage. Recovery from photoinhibition was incomplete in darkness, whereas light as low as 0·2 or 1·0 µmol m−2 s−1 resulted in complete recovery if the recovery period was long enough. Additionally, low intensity light given for1·5 h after one day in darkness caused rapid and complete recovery. In conclusion, before measuring maximal PSII efficiency (Fv/Fm) with chlorophyll fluorescence, it is important to let lichens recover in low intensity light and not in darkness, to optimize recovery from photoinhibition; dark adaptation can only be recommended if the photoinhibition status of the lichens is of interest.

Keywords

D1 proteinLobaria pulmonariaLobaria scrobiculataParmelia sulcataphotosystem IIXanthoria parietina
Type
Articles
Information
The Lichenologist , Volume 50 , Issue 1 , January 2018 , pp. 139 - 145
Copyright
© British Lichen Society, 2018 

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