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Low-light recovery effects on assessment of photoinhibition with chlorophyll fluorescence in lichens

Published online by Cambridge University Press:  26 January 2018

Knut Asbjørn SOLHAUG
Knut Asbjørn SOLHAUG*
Affiliation:
Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, P. O. Box 5003, 1432 Ås, Norway. Email: knut.solhaug@nmbu.no
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Abstract

Chlorophyll a fluorescence is often used to estimate various types of damage in lichens. In order to optimize the output and improve interpretations of such measurements the protocol for pretreatment and measuring is important. To study the effects of measurement conditions, the lichens Lobaria pulmonaria, L. scrobiculata, Xanthoria parietina and Parmelia sulcata were first stressed by high light intensities at 600 or 1000 µmol photons m−2 s−1 for 4 h. Then various conditions during recovery or pretreatment were used to optimize the detection of more lasting damage. Recovery from photoinhibition was incomplete in darkness, whereas light as low as 0·2 or 1·0 µmol m−2 s−1 resulted in complete recovery if the recovery period was long enough. Additionally, low intensity light given for1·5 h after one day in darkness caused rapid and complete recovery. In conclusion, before measuring maximal PSII efficiency (Fv/Fm) with chlorophyll fluorescence, it is important to let lichens recover in low intensity light and not in darkness, to optimize recovery from photoinhibition; dark adaptation can only be recommended if the photoinhibition status of the lichens is of interest.

Keywords

D1 proteinLobaria pulmonariaLobaria scrobiculataParmelia sulcataphotosystem IIXanthoria parietina
Type
Articles
Information
The Lichenologist , Volume 50 , Issue 1 , January 2018 , pp. 139 - 145
Copyright
© British Lichen Society, 2018 

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References

Adams, W. W. III, Demmig-Adams, B., Rosenstiel, T. N. & Ebbert, V. (2001) Dependence of photosynthesis and energy dissipation activity upon growth form and light environment during the winter. Photosynthesis Research 67: 51–62.Google Scholar
Adams, W. W. III, Muller, O., Cohu, C. M. & Demmig-Adams, B. (2013) May photoinhibition be a consequence, rather than a cause, of limited plant productivity? Photosynthesis Research 117: 31–44.Google Scholar
Aro, E.-M., McCaffery, S. & Anderson, J. M. (1994) Recovery from photoinhibition in peas (Pisum sativum L.) acclimated to varying growth irradiances: role of D1 protein turnover. Plant Physiology 104: 1033–1041.Google Scholar
Bačkor, M., Swanson, A. K. & Fahselt, D. (2006) Chlorophyll a fluorescence and photosynthetic pigment composition of three Umbilicaria lichen species in relation to cliff light microenvironment. Journal of the Hattori Botanical Laboratory 99: 297–305.Google Scholar
Baker, N. R. (2008) Chlorophyll fluorescence: a probe of photosynthesis in vivo . Annual Review of Plant Biology 59: 89–113.Google Scholar
Barták, M., Solhaug, K. A., Vráblíková, H. & Gauslaa, Y. (2006) Curling during desiccation protects the foliose lichen Lobaria pulmonaria against photoinhibition. Oecologia 149: 553–560.Google Scholar
Binder, A. (1982) Respiration and photosynthesis in energy-transducing membranes of cyanobacteria. Journal of Bioenergetics and Biomembranes 14: 271–286.Google Scholar
Campbell, D., Hurry, V., Clarke, A. K., Gustafsson, P. & Öquist, G. (1998) Chlorophyll fluorescence analysis of cyanobacterial photosynthesis and acclimation. Microbiology and Molecular Biology Reviews 62: 667–683.Google Scholar
Demmig-Adams, B., Adams, W. W. III, Czygan, F. C., Schreiber, U. & Lange, O. L. (1990 a) Differences in the capacity for radiationless energy dissipation in the photochemical apparatus of green and blue-green algal lichens associated with differences in carotenoid composition. Planta 180: 582–589.Google Scholar
Demmig-Adams, B., Máguas, C., Adams, W. W. III, Meyer, A., Kilian, E. & Lange, O. L. (1990 b) Effect of high light on the efficiency of photochemical energy conversion in a variety of lichen species with green and blue-green phycobionts. Planta 180: 400–409.Google Scholar
Färber, L., Solhaug, K. A., Esseen, P. A., Bilger, W. & Gauslaa, Y. (2014) Sunscreening fungal pigments influence the vertical gradient of pendulous lichens in boreal forest canopies. Ecology 95: 1464–1471.Google Scholar
Gauslaa, Y. & Solhaug, K. A. (1996) Differences in the susceptibility to light stress between epiphytic lichens of ancient and young boreal forest stands. Functional Ecology 10: 344–354.Google Scholar
Gauslaa, Y. & Solhaug, K. A. (2001) Fungal melanins as a sun screen for symbiotic green algae in the lichen Lobaria pulmonaria . Oecologia 126: 462–471.CrossRefGoogle ScholarPubMed
Gauslaa, Y., Coxson, D. & Solhaug, K. A. (2012) The paradox of higher light tolerance during desiccation in rare old forest cyanolichens than in more widespread co-occurring chloro- and cephalolichens. New Phytologist 195: 812–822.Google Scholar
Green, T. G. A., Nash, T. H. III & Lange, O. L. (2008) Physiological ecology of carbon dioxide exchange. In Lichen Biology (T. H. Nash III, ed.): 152–181. Cambridge: Cambridge University Press.Google Scholar
Greer, D. H., Berry, J. A. & Bjorkman, O. (1986) Photoinhibition of photosynthesis in intact bean leaves: role of light and temperature, and requirement for chloroplast-protein synthesis during recovery. Planta 168: 253–260.Google Scholar
Hanelt, D., Huppertz, K. & Nultsch, W. (1992) Photoinhibition of photosynthesis and its recovery in red algae. Botanica Acta 105: 278–284.CrossRefGoogle Scholar
Heber, U., Bilger, W., Turk, R. & Lange, O. L. (2010) Photoprotection of reaction centres in photosynthetic organisms: mechanisms of thermal energy dissipation in desiccated thalli of the lichen Lobaria pulmonaria . New Phytologist 185: 459–470.Google Scholar
Maxwell, K. & Johnson, G. N. (2000) Chlorophyll fluorescence – a practical guide. Journal of Experimental Botany 51: 659–668.Google Scholar
Nath, K., Jajoo, A., Poudyal, R. S., Timilsina, R., Park, Y. S., Aro, E.-M., Nam, H. G. & Lee, C. H. (2013) Towards a critical understanding of the photosystem II repair mechanism and its regulation during stress conditions. FEBS Letters 587: 3372–3381.Google Scholar
Osmond, C. B. (1994) What is photoinhibition? Some insights from comparisons of shade and sun plants. In Photoinhibition of Photosynthesis From Molecular Mechanisms to the Field (N. R. Baker, ed.): 1–24. Oxford: BIOS Scientific Publishers Ltd.Google Scholar
Singh, M., Yamamoto, Y., Satoh, K., Aro, E.-M. & Kanervo, E. (2005) Post-illumination-related loss of photochemical efficiency of Photosystem II and degradation of the D1 protein are temperature-dependent. Journal of Plant Physiology 162: 1246–1253.Google Scholar
Singh, M., Satoh, K., Yamamoto, Y., Kanervo, E. & Aro, E.-M. (2008) In vivo quality control of photosystem II in cyanobacteria, Synechocystis sp. PCC 6803: D1 protein degradation and repair under the influence of light, heat and darkness. Indian Journal of Biochemistry and Biophysics 45: 237–243.Google Scholar
Solhaug, K. A., Xie, L. & Gauslaa, Y. (2014) Unequal allocation of excitation energy between photosystem II and I reduces cyanolichen photosynthesis in blue light. Plant and Cell Physiology 55: 1404–1414.CrossRefGoogle ScholarPubMed
Vonshak, A., Torzillo, G. & Tomaseli, L. (1994) Use of chlorophyll fluorescence to estimate the effect of photoinhibition in outdoor cultures of Spirulina platensis . Journal of Applied Phycology 6: 31–34.Google Scholar
Vráblíková, H., McEvoy, M., Solhaug, K. A., Barták, M. & Gauslaa, Y. (2006) Annual variation in photoacclimation and photoprotection of the photobiont in the foliose lichen Xanthoria parietina . Journal of Photochemistry and Photobiology B: Biology 83: 151–162.Google Scholar