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Morphological and nuclear ribosomal DNA data support distinguishing two new species of Umbilicaria (Umbilicariaceae, Ascomycota) from Europe

Published online by Cambridge University Press:  23 September 2009

Beata KRZEWICKA ,
Miguel A. GARCÍA ,
Steinar D. JOHANSEN ,
Leopoldo G. SANCHO  and
María P. MARTÍN
Beata KRZEWICKA
Affiliation:
Laboratory of Lichenology, Institute of Botany Polish Academy of Sciences, Lubicz 46, PL–31–512 Kraków, Poland. Email: b.krzewicka@botany.pl
Miguel A. GARCÍA
Affiliation:
Real Jardín Botánico, CSIC, Plaza de Murillo 2, 28014 Madrid, Spain.
Steinar D. JOHANSEN
Affiliation:
Department of Molecular Biotechnology, Institute of Medical Biology, University of Tromsø, N–9037 Tromsø, Norway.
Leopoldo G. SANCHO
Affiliation:
Departamento de Biología Vegetal II, Facultad de Farmacia, Universidad Camplutense, E–28040 Madrid, Spain.
María P. MARTÍN
Affiliation:
Real Jardín Botánico, CSIC, Plaza de Murillo 2, 28014 Madrid, Spain.
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Abstract

Umbilicaria iberica and U. maculata are described as new to science on the basis of morphological and molecular data. Umbilicaria iberica is similar to U. polyphylla but differs in having a monophyllous thallus with a distinctive white reticular pattern over the umbilicus and actinodisc apothecia. Umbilicaria maculata is similar to U. cylindrica but this new species is distinguished by its grey-brown thallus with sparse marginal cilia and white stains on the upper surface especially in the marginal zone, as well as by sessile apothecia with one or a few sterile fissures. Nuclear ITS and LSU rDNA have been used as molecular markers. In the phylogenetic analysis U. polyphylla falls into two well supported clades (A and B), one of which corresponds to the morphotype that is described here as a new taxon. Specimens previously recognized as U.cylindrica fall into three well supported clades: clade C corresponds to the typical morphotype, clade D corresponds to morphotype II described here as a new taxon, and clade E is morphotype III which is recognized as U. cf. umbilicarioides. Typical group I introns have been found in position 1506 of the nuclear SSU rDNA of Umbilicaria; a consensus secondary structure of these introns is presented and we conclude that they represent an important and valuable molecular marker which gives additional support to the ITS and LSU sequence phylogeny obtained.

Keywords

new speciesUmbilicaria ibericaUmbilicaria maculataITS and LSUintrons
Type
Research Article
Information
The Lichenologist , Volume 41 , Issue 6 , November 2009 , pp. 631 - 648
Copyright
Copyright © British Lichen Society 2009

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