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Conserving original in situ diversity in microzooplankton grazing set-ups

Published online by Cambridge University Press:  15 March 2010

Martin Günter Joachim Löder ,
Nicole Aberle ,
Christine Klaas ,
Alexandra Claudia Kraberg  and
Karen Helen Wiltshire
Martin Günter Joachim Löder*
Affiliation:
Biologische Anstalt Helgoland, Alfred-Wegener-Institute for Polar and Marine Research, Marine Station, POB 180, 27483 Helgoland, Germany
Nicole Aberle
Affiliation:
Biologische Anstalt Helgoland, Alfred-Wegener-Institute for Polar and Marine Research, Marine Station, POB 180, 27483 Helgoland, Germany
Christine Klaas
Affiliation:
Alfred-Wegener-Institute for Polar and Marine Research, POB 12 01 61, 27515 Bremerhaven, Germany
Alexandra Claudia Kraberg
Affiliation:
Biologische Anstalt Helgoland, Alfred-Wegener-Institute for Polar and Marine Research, Marine Station, POB 180, 27483 Helgoland, Germany
Karen Helen Wiltshire
Affiliation:
Biologische Anstalt Helgoland, Alfred-Wegener-Institute for Polar and Marine Research, Marine Station, POB 180, 27483 Helgoland, Germany
*
Correspondence should be addressed to: M.G.J. Löder, Biologische Anstalt Helgoland, Alfred-Wegener-Institute for Polar and Marine Research, Marine Station, POB 180, 27483 Helgoland, Germany email: Martin.Loeder@awi.de
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Abstract

Grazing experiments targeting the determination of in situ grazing rates are standard. In two separate experiments the effect of the frequently used siphon filling technique on the abundance of microzooplankton during the set-up of grazing experiments was investigated and compared to results from an alternative filling method. Hereby, water containing natural communities from Helgoland Roads, Germany (54°11.3′N 7°54.0′E), was transferred into incubation bottles using a funnel system (funnel-transfer technique (FTT)). The impact of pre-screening with a 200 µm net for excluding larger mesozooplankton grazers from the incubations was evaluated. Results show that the ciliate community was strongly affected by siphoning and pre-screening, leading to significant differences in abundance and Margalef diversity. The most affected ciliates were Lohmanniella oviformis and Myrionecta rubra, both important species in the North Sea. Dinoflagellates did not show any significant response to either siphoning or pre-screening with the exception of one athecate species. Such artificial bias in ciliate assemblages is very problematic for biodiversity consideration and grazing investigations. Simply changing the method of filling during the experimental set-up can ensure the measurement of accurate grazing rates of field abundances of microzooplankton. We thus recommend using conservative filling approaches like the FTT in experiments, especially when sensitive species are present, in order to avoid shifts in the overall microzooplankton community. Furthermore, we recommend introducing a control to evaluate the degree of changes in the target community due to the experimental set-up.

Keywords

ciliatesdilution experimentsdinoflagellatesFTTmicrozooplanktonsiphoning
Type
Research Article
Information
Marine Biodiversity Records , Volume 3 , June 2010 , e28
Copyright
Copyright © Marine Biological Association of the United Kingdom 2010

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References

REFERENCES

Aberle, N., Lengfellner, K. and Sommer, U. (2007) Spring bloom succession, grazing impact and herbivore selectivity of ciliate communities in response to winter warming. Oecologia 150, 668–681.CrossRefGoogle ScholarPubMed
Broglio, E., Jónasdóttir, S.H., Calbet, A., Jakobsen, H.H. and Saiz, E. (2003) Effect of heterotrophic versus autotrophic food on feeding and reproduction of the calanoid copepod Acartia tonsa: relationship with prey fatty acid composition. Aquatic Microbial Ecology 31, 267–278.CrossRefGoogle Scholar
Buskey, E.J. and Stoecker, D.K. (1989) Behavioral responses of the marine tintinnid Favella sp. to phytoplankton: influence of chemical, mechanical and photic stimuli. Journal of Experimental Marine Biology and Ecology 132, 1–16.CrossRefGoogle Scholar
Calbet, A. and Landry, M.R. (2004) Phytoplankton growth, microzooplankton grazing, and carbon cycling in marine systems. Limnology and Oceanography 49, 51–57.CrossRefGoogle Scholar
Christaki, U., Dolan, J.R., Pelegri, S. and Rassoulzadegan, F. (1998) Consumption of picoplankton-size particles by marine ciliates: effects of physiological state of the ciliate and particle quality. Limnology and Oceanography 43, 458–464.CrossRefGoogle Scholar
Dodge, J.D. (1982) Marine dinoflagellates of the British Isles. London: Her Majesty's Stationery Office.Google Scholar
Drebes, G. (1974) Marines phytoplankton—Eine Auswahl der Helgoländer Planktonalgen (Diatomeen, Peridineen). Stuttgart: Georg Thieme Verlag.Google Scholar
Fenchel, T. and Hansen, P.J. (2006) Motile behaviour of the bloom-forming ciliate Mesodinium rubrum. Marine Biology Research 2, 33–40.CrossRefGoogle Scholar
Fonda Umani, S., Tirelli, V., Beran, A. and Guardiani, B. (2005) Relations between microzooplankton and mesozooplankton: competition versus predation on natural assemblages of the Gulf of Trieste (northern Adriatic Sea). Journal of Plankton Research 27, 973–986.CrossRefGoogle Scholar
Gifford, D.J. (1985) Laboratory culture of marine planktonic oligotrichs (Ciliophora, Oligotrichida). Marine Ecology Progress Series 23, 257–267.CrossRefGoogle Scholar
Jakobsen, H.H. (2001) Escape response of planktonic protists to fluid mechanical signals. Marine Ecology Progress Series 214, 67–78.CrossRefGoogle Scholar
Jakobsen, H.H. (2002) Escape of protists in predator-generated feeding currents. Aquatic Microbial Ecology 26, 271–281.CrossRefGoogle Scholar
Jakobsen, H.H., Everett, L.M. and Strom, S.L. (2006) Hydromechanical signaling between the ciliate Mesodinium pulex and motile protist prey. Aquatic Microbial Ecology 44, 197–206.CrossRefGoogle Scholar
Jonsson, P.R. (1986) Particle size selection, feeding rates and growth dynamics of marine planktonic oligotrichous ciliates (Ciliophora: Oligotrichina). Marine Ecology Progress Series 33, 265–277.CrossRefGoogle Scholar
Kahl, A. (1932) Urtiere oder Protozoa. I: Wimpertiere oder Ciliata (Infusoria). 3 Spirotricha. In Dahl, F. (ed.) Die Tierwelt Deutschlands und der angrenzenden Meeresteile nach ihrem Merkmalen und ihrer Lebensweise. Jena: Gustav Fischer Verlag, pp. 399–650. [18. Teil]Google Scholar
Kivi, K. and Setälä, O. (1995) Simultaneous measurement of food particle selection and clearance rates of planktonic oligotrich ciliates (Ciliophora: Oligotrichina). Marine Ecology Progress Series 119, 125–137.CrossRefGoogle Scholar
Landry, M.R. and Hassett, R.P. (1982) Estimating the grazing impact of marine micro-zooplankton. Marine Biology 67, 283–288.CrossRefGoogle Scholar
Liu, H. and Dagg, M. (2003) Interactions between nutrients, phytoplankton growth and micro- and mesozooplankton grazing in the plume of the Mississippi River. Marine Ecology Progress Series 258, 31–42.CrossRefGoogle Scholar
Maldonado, E.M. and Latz, M.I. (2007) Shear-stress dependence of dinoflagellates bioluminescence. Biological Bulletin. Marine Biological Laboratory, Woods Hole 212, 242–249.CrossRefGoogle ScholarPubMed
Montagnes, D.J.S. (2003) Planktonic ciliate project internet homepage. http://www.liv.ac.uk/ciliate/intro.htm. Accessed: 19 December 2007, time: 13:56.Google Scholar
Myung, G., Yih, W., Kim, H.S., Park, J.S. and Cho, B.C. (2006) Ingestion of bacterial cells by the marine photosynthetic ciliate Myrionecta rubra. Aquatic Microbial Ecology 44, 175–180.CrossRefGoogle Scholar
Park, J.S., Myung, G., Kim, H.S., Cho, B.C. and Yih, W. (2007) Growth responses of the marine photosynthetic ciliate Myrionecta rubra to different cryptomonad strains. Aquatic Microbial Ecology 48, 83–90.CrossRefGoogle Scholar
Paterson, H.L., Knott, B., Koslow, A.J. and Waite, A.M. (2008) The grazing impact of microzooplankton off south west Western Australia: as measured by the dilution technique. Journal of Plankton Research 30, 379–392.CrossRefGoogle Scholar
Pearce, I., Davidson, A.T., Wright, S. and Van den Enden, R. (2008) Seasonal changes in phytoplankton growth and microzooplankton grazing at an Antarctic coastal site. Aquatic Microbial Ecology 50, 157–167.CrossRefGoogle Scholar
Robinson, H.E., Finelli, C.M. and Buskey, E.J. (2007) The turbulent life of copepods: effects of water flow over a coral reef on their ability to detect and evade predators. Marine Ecology Progress Series 349, 171–181.CrossRefGoogle Scholar
Sakka Hlaili, A., Grami, B., Hadj Mabrouk, H., Gosselin, M. and Hamel, D. (2007) Phytoplankton growth and microzooplankton grazing rates in a restricted Mediterranean lagoon (Bizerte Lagoon, Tunisia). Marine Biology 151, 767–783.CrossRefGoogle Scholar
Sherr, E.B. and Sherr, B.F. (2007) Heterotrophic dinoflagellates: a significant component of microzooplankton biomass and major grazers of diatoms in the sea. Marine Ecology Progress Series 352, 187–197.CrossRefGoogle Scholar
Singarajah, K.V. (1969) Escape reactions of zooplankton: the avoidance of a pursing siphon tube. Journal of Experimental Marine Biology and Ecology 3, 171–178.CrossRefGoogle Scholar
Smetacek, V. (1984) Growth dynamics of a common Baltic protozooplankter: the ciliate genus Lohmanniella. Limnologica 15, 371–376.Google Scholar
Stelfox-Widdicombe, C.E., Archer, S.D., Burkill, P.H. and Stefels, J. (2004) Microzooplankton grazing in Phaeocystis and diatom-dominated waters in the southern North Sea in spring. Journal of Sea Research 51, 37–51.CrossRefGoogle Scholar
Stoecker, D.K., Michaels, A.E. and Davis, L.H. (1987) Large proportion of marine planktonic ciliates found to contain functional chloroplasts. Nature 326, 790–792.CrossRefGoogle Scholar
Strom, S.L., Macri, E.L. and Olson, M.B. (2007) Microzooplankton grazing in the coastal Gulf of Alaska: variations in top-down control of phytoplankton. Limnology and Oceanography 52, 1480–1494.CrossRefGoogle Scholar
Suzuki, K., Tsuda, A., Kiyosawa, H., Takeda, S., Nishioka, J., Saino, T., Takahashi, M. and Wong, C.S. (2002) Grazing impact of microzooplankton on a diatom bloom in a mesocosm as estimated by pigment-specific dilution technique. Journal of Experimental Marine Biology and Ecology 271, 99–120.CrossRefGoogle Scholar
Throndsen, J. (1978) Preservation and storage. In Sournia, A. (ed.) Phytoplankton manual. Paris: UNESCO, pp. 69–74.Google Scholar
Tillmann, U. (2004) Interactions between planktonic microalgae and protozoan grazers. Journal of Eukaryotic Microbiology 51, 156–168.CrossRefGoogle ScholarPubMed
Titelman, J. and Kiørboe, T. (2003) Predator avoidance by nauplii. Marine Ecology Progress Series 247, 137–149.CrossRefGoogle Scholar
Tomas, C.R. (1996) Identifying marine diatoms and dinoflagellates. San Diego, New York, Boston, London, Tokyo, Toronto: Academic Press Inc.Google Scholar
Utermöhl, H. (1958) Zur Vervollkommnung der quantitativen Plankton-Methodik. Mitteilung der Internationalen Vereinigung für Theoretische und Angewandte Limnologie 9, 1–38.Google Scholar
Washington, H.G. (1984) Diversity, biotic and similarity indices—a review with special relevance to aquatic ecosystems. Water Research 18, 653–694.CrossRefGoogle Scholar
Wiltshire, K.H., Harsdorf, S., Smidt, B., Blöcker, G., Reuter, R. and Schroeder, F. (1998) The determination of algal biomass (as chlorophyll) in suspended matter from the Elbe estuary and the German Bight: a comparison of high-performance liquid chromatography, delayed fluorescence and prompt fluorescence methods. Journal of Experimental Marine Biology and Ecology 222, 113–131.CrossRefGoogle Scholar
Wiltshire, K.H., Boersma, M., Möller, A. and Buhtz, H. (2000) Extraction of pigments and fatty acids from the green alga Scenedesmus obliquus (Chlorophyceae). Aquatic Ecology 34, 119–126.CrossRefGoogle Scholar
Wiltshire, K.H., Malzahn, A.M., Wirtz, K., Greve, W., Janisch, S., Mangelsdorf, P., Manly, B.F., Boersma, M. (2008) Resilience of North Sea phytoplankton spring bloom dynamics: An analysis of the long-term data at Helgoland Roads. Limnology and Oceanography 53, 1294–1302.CrossRefGoogle Scholar