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Data-driven Sliding Mode Control for Pulses of Fluorescence in STED Microscopy Based on Förster Resonance Energy Transfer Pairs

Published online by Cambridge University Press:  13 January 2020

Maison Clouatre  and
Makhin Thitsa
Maison Clouatre
Affiliation:
Department of Electrical and Computer Engineering, Mercer University, Mercer University Drive, Macon, GA 31207, USA
Makhin Thitsa*
Affiliation:
Department of Electrical and Computer Engineering, Mercer University, Mercer University Drive, Macon, GA 31207, USA
*
*(Email: thitsa_m@mercer.edu)
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Abstract

Pairs of conjugate donor-acceptor fluorescent probes have proven themselves useful in stimulated emission depletion (STED) microscopy in recent years. For instance, it has been shown that the lifetime of said probes directly correlates to the resolution of the microscope. However, once the lifetimes of the probes have been optimized, it is desirable to control their fluorescence in order to improve the resolution further. Here, we propose combining model-free control with sliding mode control to track nanosecond pulses of red-shifted acceptor fluorescence in order to inhibit visible light emitted from the image plane, shrink the point spread function, and subsequently improve the resolution of the microscope. This is achieved by automatic adjustment of the STED laser beam pump power. This controller is numerically simulated against a generic model created from Förster resonance energy transfer (FRET) theory. However, since it is data-driven, it can be easily applied to various physical systems with drastically different dynamics. This work provides a reliable theoretic control solution to modern super resolution microscopy for biological imaging.

Keywords

laseroptical propertiesfunctionalactuation
Type
Articles
Information
MRS Advances , Volume 5 , Issue 29-30: Materials Theory, Computation and Characterization , 2020 , pp. 1557 - 1565
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Copyright
Copyright © Materials Research Society 2020

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