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Static and dynamic behavior of aggrecan solutions

Published online by Cambridge University Press:  23 December 2019

Ferenc Horkay ,
Peter J. Basser  and
Erik Geissler
Ferenc Horkay*
Affiliation:
Section on Quantitative Imaging and Tissue Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 49 Convent Drive, Bethesda, MD 20892-5772, USA
Peter J. Basser
Affiliation:
Section on Quantitative Imaging and Tissue Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 49 Convent Drive, Bethesda, MD 20892-5772, USA
Erik Geissler
Affiliation:
Université Grenoble Alpes, Laboratoire Interdisciplinaire de Physique, and CNRS, LIPhy, F-38402 St Martin d’Hères cedex, France
*
*(Email: horkayf@helix.nih.gov)
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Abstract

Small angle neutron scattering (SANS) and dynamic light scattering (DLS) measurements were made on near physiological solutions of a bottlebrush shape polyelectrolyte, aggrecan. Aggrecan is a biologically important molecule whose complexes with hyaluronic acid (HA) provide the osmotic resistance of cartilage. We have investigated the effect of complexation of aggrecan with HA on the structure and dynamic properties of aggrecan solutions. SANS reveals that the supramolecular structure of aggrecan assemblies is only marginally affected by the HA. DLS indicates that the dynamic response of the aggrecan-HA complex is slower than that of the corresponding aggrecan solution. However, addition of calcium ions slightly increases the relaxation rate of the autocorrelation function of the aggrecan solution.

Keywords

neutron scatteringbiomimeticmicrostructureCa
Type
Articles
Information
MRS Advances , Volume 5 , Issue 17: Soft Materials and Biomaterials , 2020 , pp. 891 - 897
Copyright
Copyright © Materials Research Society 2019

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